Detailed information NTG0020670

Bioinformatically predicted

Overview

• Name: pilR
• Type: Regulator
• Locus tag: PA4547
• Genome accession: NC_002516
• Coordinates: 5094985..5096322 (+)
• Length: 445 a.a.
• NCBI ID: NP_253237.1
• Uniprot ID: Q00934
• Organism: Pseudomonas aeruginosa PAO1
• Function: regulate pilin expression (predicted from homology) Competence regulation

Genomic Context

Location: 5089985..5101322

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PA4543	-	5090128..5090856 (-)	729	NP_253233.1	hypothetical protein	-
PA4544	rluD	5090853..5091815 (-)	963	NP_253234.1	pseudouridine synthase	-
PA4545	comL	5091961..5092986 (+)	1026	NP_253235.1	competence protein ComL	-
PA4546	pilS	5093378..5094970 (+)	1593	NP_253236.1	two-component sensor PilS	Regulator
PA4547	pilR	5094985..5096322 (+)	1338	NP_253237.1	two-component response regulator PilR	Regulator
PA4548	-	5096368..5097462 (-)	1095	NP_253238.1	D-amino acid oxidase	-
PA4549	fimT	5097597..5098106 (+)	510	NP_253239.1	type 4 fimbrial biogenesis protein FimT	-
PA4550	fimU	5098212..5098718 (+)	507	NP_253240.1	type 4 fimbrial biogenesis protein FimU	-
PA4551	pilV	5098709..5099266 (+)	558	NP_253241.1	type 4 fimbrial biogenesis protein PilV	Machinery gene
PA4552	pilW	5099263..5100087 (+)	825	NP_253242.1	type 4 fimbrial biogenesis protein PilW	-
PA4553	pilX	5100084..5100671 (+)	588	NP_253243.1	type 4 fimbrial biogenesis protein PilX	-

Sequence

Protein

• Length: 445 a.a.
• Molecular weight: 49737.06 Da
• Isoelectric Point: 5.8654

>NTDB_id=20670 PA4547 NP_253237.1 5094985..5096322(+) (pilR) [Pseudomonas aeruginosa PAO1]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLAERI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide

• Length: 1338 bp

>NTDB_id=20670 PA4547 NP_253237.1 5094985..5096322(+) (pilR) [Pseudomonas aeruginosa PAO1]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGTAGCGGCAAGGAGCTGGTGGCGCGCCTGATCCACGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAACTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGCGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGAACGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAGAAGCTGAAGAACTACCG
CTTCCCGGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTACACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCCGCGAGCCTGAGCGAAATCGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCGCTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

• Response regulator receiver domain (Response_reg) (7-115)

• Sigma-54 interaction domain (Sigma54_activat) (135-301)

• Bacterial regulatory protein, Fis family (HTH_8) (402-442)

3D structure

• AlphaFold DB: Q00934

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilR	Pseudomonas aeruginosa PAK	100	100	1
pilR	Acinetobacter baumannii strain A118	51.198	100	0.528
luxO	Vibrio cholerae strain A1552	38.17	100	0.384