Detailed information NTG0020669

Bioinformatically predicted

Overview

• Name: pilS
• Type: Regulator
• Locus tag: PA4546
• Genome accession: NC_002516
• Coordinates: 5093378..5094970 (+)
• Length: 530 a.a.
• NCBI ID: NP_253236.1
• Uniprot ID: P33639
• Organism: Pseudomonas aeruginosa PAO1
• Function: regulate pilin expression (predicted from homology) Competence regulation

Genomic Context

Location: 5088378..5099970

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PA4543	-	5090128..5090856 (-)	729	NP_253233.1	hypothetical protein	-
PA4544	rluD	5090853..5091815 (-)	963	NP_253234.1	pseudouridine synthase	-
PA4545	comL	5091961..5092986 (+)	1026	NP_253235.1	competence protein ComL	-
PA4546	pilS	5093378..5094970 (+)	1593	NP_253236.1	two-component sensor PilS	Regulator
PA4547	pilR	5094985..5096322 (+)	1338	NP_253237.1	two-component response regulator PilR	Regulator
PA4548	-	5096368..5097462 (-)	1095	NP_253238.1	D-amino acid oxidase	-
PA4549	fimT	5097597..5098106 (+)	510	NP_253239.1	type 4 fimbrial biogenesis protein FimT	-
PA4550	fimU	5098212..5098718 (+)	507	NP_253240.1	type 4 fimbrial biogenesis protein FimU	-
PA4551	pilV	5098709..5099266 (+)	558	NP_253241.1	type 4 fimbrial biogenesis protein PilV	Machinery gene

Sequence

Protein

• Length: 530 a.a.
• Molecular weight: 58985.16 Da
• Isoelectric Point: 6.6886

>NTDB_id=20669 PA4546 NP_253236.1 5093378..5094970(+) (pilS) [Pseudomonas aeruginosa PAO1]
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEDQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLMLCGLFYAGGGVPSGIGSLLVVAVAIANILLRGRIGLVIAAAASLGLLYLTFFLSLSSPDATNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSRQAILLANQAALGLLRQDDVQGASLG
RHSPMLMHCMKQWRLNPSLRPPTLKVVPDGPTVQPSFISLNREDDQHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDAPDRRLTQIIQDQSKRMNLVIENVLQLSRRRQAEPQQLDLKEWLQRFVDEYPGRLRND
SQLHLQLGAGDIQTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLARDPESDLPVLEVIDDGPGVPADKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS

Nucleotide

• Length: 1593 bp

>NTDB_id=20669 PA4546 NP_253236.1 5093378..5094970(+) (pilS) [Pseudomonas aeruginosa PAO1]
GTGCGCGCTGAACGGCTACGGCTGAGCGAGGAGCAGGGGCAACGCATCCTCCGTCTGTACCACCTGTACCGCCTGACCAT
CGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAACTGGAAGATCAGGTCCTCAAGCTCGTCCACCCTGAACTGTTCCATG
TCGGCAGTTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCGCCGTCACGGCAATTGCTGCCGATCTTC
ATCCTCGCGCTCACCGACGTGCTGATGCTTTGCGGCCTGTTCTACGCAGGTGGCGGCGTACCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTGGCCATTGCCAACATCCTGCTGCGCGGGCGCATCGGCCTGGTCATCGCGGCGGCGGCCAGCCTCG
GCCTGCTCTACCTGACCTTCTTCCTCAGCCTGAGCAGTCCGGACGCCACCAACCACTACGTCCAGGCCGGCGGCCTCGGC
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCTCTGGTGCGGCGCCAGGAGCAGACCGAAACGCTGGCCGAAGAACG
CGCCGAGACGGTCGCCAACCTGGAGGAACTCAACGCATTGATCCTGCAGCGCATGCGCACCGGCATCCTCGTGGTCGATA
GCCGTCAGGCCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACGACGTGCAGGGCGCCAGCCTCGGC
CGCCACAGCCCGATGCTGATGCACTGCATGAAGCAATGGCGCCTGAATCCCAGCCTCCGTCCGCCGACGCTCAAGGTGGT
GCCGGATGGCCCGACGGTGCAACCCAGCTTTATCAGCCTCAACCGCGAAGACGACCAGCACGTGCTGATCTTCCTCGAAG
ACATTTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTGGCCGGTCTCGGCCGCCTGACCGCCGGCATCGCCCATGAG
ATCCGCAACCCGCTGGGCGCGATCAGCCACGCCGCCCAACTGCTGCAGGAGTCAGAGGAACTGGATGCCCCGGACCGACG
CCTGACGCAGATCATCCAGGACCAGTCGAAGCGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGTCGCCGCC
AGGCCGAACCGCAGCAGCTCGACCTGAAGGAGTGGCTTCAGCGGTTCGTCGACGAATACCCCGGCAGGCTGCGCAACGAC
AGCCAACTGCACCTGCAGCTCGGTGCCGGCGACATCCAGACCCGCATGGACCCACATCAGTTGAACCAGGTGCTGAGCAA
CCTGGTGCAGAACGGTCTTCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGCCTCGCGCGCGACCCGG
AGAGCGACCTGCCGGTGCTGGAAGTCATCGACGACGGTCCCGGCGTACCGGCGGACAAACTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAGCTCTGCGAGAGCAACCAGGCACGGAT
CGACTACCGCAATCGCGAGGAAGGCGGCGGCTGCTTCCGCATCACCTTCGCCCACCCACGCAAACTCAGCTGA

Domains

Predicted by InterproScan.

• Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c) (417-523)

• His Kinase A (phospho-acceptor) domain (HisKA) (310-373)

3D structure

• AlphaFold DB: P33639

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilS	Pseudomonas aeruginosa PAK	99.623	100	0.996