Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BSF20_RS14565 Genome accession   NZ_CP018152
Coordinates   2790993..2792063 (+) Length   356 a.a.
NCBI ID   WP_057080484.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain LM2303     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2785993..2797063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSF20_RS14535 (BSF20_14240) - 2786462..2786707 (-) 246 WP_003153078.1 DUF2626 domain-containing protein -
  BSF20_RS14540 (BSF20_14245) - 2786944..2787324 (+) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  BSF20_RS14550 (BSF20_14255) - 2787530..2788360 (+) 831 WP_003153080.1 STAS domain-containing protein -
  BSF20_RS14555 (BSF20_14260) - 2788398..2789699 (-) 1302 WP_014305416.1 hemolysin family protein -
  BSF20_RS14560 (BSF20_14265) - 2789845..2790795 (+) 951 WP_003153082.1 magnesium transporter CorA family protein -
  BSF20_RS14565 (BSF20_14270) comGA 2790993..2792063 (+) 1071 WP_057080484.1 competence type IV pilus ATPase ComGA Machinery gene
  BSF20_RS14570 (BSF20_14275) comGB 2792050..2793087 (+) 1038 WP_014305414.1 competence type IV pilus assembly protein ComGB Machinery gene
  BSF20_RS14575 (BSF20_14280) comGC 2793092..2793400 (+) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  BSF20_RS14580 (BSF20_14285) comGD 2793390..2793827 (+) 438 WP_072588444.1 competence type IV pilus minor pilin ComGD Machinery gene
  BSF20_RS14585 (BSF20_14290) comGE 2793811..2794125 (+) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  BSF20_RS14590 (BSF20_14295) comGF 2794139..2794534 (+) 396 WP_003153090.1 competence type IV pilus minor pilin ComGF -
  BSF20_RS14595 (BSF20_14300) comGG 2794535..2794912 (+) 378 WP_014305410.1 competence type IV pilus minor pilin ComGG Machinery gene
  BSF20_RS14600 (BSF20_14305) - 2794969..2795148 (+) 180 WP_003153093.1 YqzE family protein -
  BSF20_RS14605 (BSF20_14310) - 2795188..2795517 (-) 330 WP_003153097.1 DUF3889 domain-containing protein -
  BSF20_RS14610 (BSF20_14315) tapA 2795776..2796447 (+) 672 WP_003153099.1 amyloid fiber anchoring/assembly protein TapA -
  BSF20_RS14615 (BSF20_14320) sipW 2796419..2797003 (+) 585 WP_003153100.1 signal peptidase I SipW -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40552.89 Da        Isoelectric Point: 9.4731

>NTDB_id=206545 BSF20_RS14565 WP_057080484.1 2790993..2792063(+) (comGA) [Bacillus amyloliquefaciens strain LM2303]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALLKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFHEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=206545 BSF20_RS14565 WP_057080484.1 2790993..2792063(+) (comGA) [Bacillus amyloliquefaciens strain LM2303]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTTTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGTTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGTTCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAAACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACCCGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCATGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.123

95.787

0.365

Multiple sequence alignment