Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   BSF20_RS14575 Genome accession   NZ_CP018152
Coordinates   2793092..2793400 (+) Length   102 a.a.
NCBI ID   WP_003153087.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain LM2303     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2788092..2798400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSF20_RS14555 (BSF20_14260) - 2788398..2789699 (-) 1302 WP_014305416.1 hemolysin family protein -
  BSF20_RS14560 (BSF20_14265) - 2789845..2790795 (+) 951 WP_003153082.1 magnesium transporter CorA family protein -
  BSF20_RS14565 (BSF20_14270) comGA 2790993..2792063 (+) 1071 WP_057080484.1 competence type IV pilus ATPase ComGA Machinery gene
  BSF20_RS14570 (BSF20_14275) comGB 2792050..2793087 (+) 1038 WP_014305414.1 competence type IV pilus assembly protein ComGB Machinery gene
  BSF20_RS14575 (BSF20_14280) comGC 2793092..2793400 (+) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  BSF20_RS14580 (BSF20_14285) comGD 2793390..2793827 (+) 438 WP_072588444.1 competence type IV pilus minor pilin ComGD Machinery gene
  BSF20_RS14585 (BSF20_14290) comGE 2793811..2794125 (+) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  BSF20_RS14590 (BSF20_14295) comGF 2794139..2794534 (+) 396 WP_003153090.1 competence type IV pilus minor pilin ComGF -
  BSF20_RS14595 (BSF20_14300) comGG 2794535..2794912 (+) 378 WP_014305410.1 competence type IV pilus minor pilin ComGG Machinery gene
  BSF20_RS14600 (BSF20_14305) - 2794969..2795148 (+) 180 WP_003153093.1 YqzE family protein -
  BSF20_RS14605 (BSF20_14310) - 2795188..2795517 (-) 330 WP_003153097.1 DUF3889 domain-containing protein -
  BSF20_RS14610 (BSF20_14315) tapA 2795776..2796447 (+) 672 WP_003153099.1 amyloid fiber anchoring/assembly protein TapA -
  BSF20_RS14615 (BSF20_14320) sipW 2796419..2797003 (+) 585 WP_003153100.1 signal peptidase I SipW -
  BSF20_RS14620 (BSF20_14325) tasA 2797067..2797852 (+) 786 WP_003153102.1 biofilm matrix protein TasA -
  BSF20_RS14625 (BSF20_14330) sinR 2797900..2798235 (-) 336 WP_003153104.1 transcriptional regulator SinR Regulator

Sequence

Protein


Download         Length: 102 a.a.        Molecular weight: 11381.28 Da        Isoelectric Point: 6.2096

>NTDB_id=206547 BSF20_RS14575 WP_003153087.1 2793092..2793400(+) (comGC) [Bacillus amyloliquefaciens strain LM2303]
MLRLKNQDGFTLIEMLIVLFIVSILLLITIPNVTKHNQSIQHKGCEGLQNMVKAQVTAYEIDHEGKMPDMNDLQSEGYIK
KNTACPNGKQILISGGEVTVEQ

Nucleotide


Download         Length: 309 bp        

>NTDB_id=206547 BSF20_RS14575 WP_003153087.1 2793092..2793400(+) (comGC) [Bacillus amyloliquefaciens strain LM2303]
GTGCTTCGTCTGAAAAATCAAGATGGATTTACTTTAATTGAAATGCTGATCGTTTTATTTATCGTTTCCATTCTGCTTTT
AATCACCATTCCTAACGTTACAAAACATAATCAAAGCATTCAGCATAAAGGCTGTGAAGGACTGCAGAATATGGTGAAAG
CCCAAGTGACGGCGTACGAAATCGACCATGAAGGTAAAATGCCGGATATGAACGATTTACAATCAGAGGGGTATATCAAA
AAGAATACAGCCTGCCCGAATGGTAAACAGATCTTAATTAGTGGCGGAGAAGTTACAGTTGAACAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

77.083

94.118

0.725
  comGC Staphylococcus aureus MW2

42.708

94.118

0.402
  comGC Staphylococcus aureus N315

42.708

94.118

0.402

Multiple sequence alignment