Detailed information NTG0206548

Bioinformatically predicted

Overview

• Name: comGD
• Type: Machinery gene
• Locus tag: BSF20_RS14580
• Genome accession: NZ_CP018152
• Coordinates: 2793390..2793827 (+)
• Length: 145 a.a.
• NCBI ID: WP_072588444.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain LM2303
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2788390..2798827

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSF20_RS14555 (BSF20_14260)	-	2788398..2789699 (-)	1302	WP_014305416.1	hemolysin family protein	-
BSF20_RS14560 (BSF20_14265)	-	2789845..2790795 (+)	951	WP_003153082.1	magnesium transporter CorA family protein	-
BSF20_RS14565 (BSF20_14270)	comGA	2790993..2792063 (+)	1071	WP_057080484.1	competence type IV pilus ATPase ComGA	Machinery gene
BSF20_RS14570 (BSF20_14275)	comGB	2792050..2793087 (+)	1038	WP_014305414.1	competence type IV pilus assembly protein ComGB	Machinery gene
BSF20_RS14575 (BSF20_14280)	comGC	2793092..2793400 (+)	309	WP_003153087.1	competence type IV pilus major pilin ComGC	Machinery gene
BSF20_RS14580 (BSF20_14285)	comGD	2793390..2793827 (+)	438	WP_072588444.1	competence type IV pilus minor pilin ComGD	Machinery gene
BSF20_RS14585 (BSF20_14290)	comGE	2793811..2794125 (+)	315	WP_015388003.1	competence type IV pilus minor pilin ComGE	Machinery gene
BSF20_RS14590 (BSF20_14295)	comGF	2794139..2794534 (+)	396	WP_003153090.1	competence type IV pilus minor pilin ComGF	-
BSF20_RS14595 (BSF20_14300)	comGG	2794535..2794912 (+)	378	WP_014305410.1	competence type IV pilus minor pilin ComGG	Machinery gene
BSF20_RS14600 (BSF20_14305)	-	2794969..2795148 (+)	180	WP_003153093.1	YqzE family protein	-
BSF20_RS14605 (BSF20_14310)	-	2795188..2795517 (-)	330	WP_003153097.1	DUF3889 domain-containing protein	-
BSF20_RS14610 (BSF20_14315)	tapA	2795776..2796447 (+)	672	WP_003153099.1	amyloid fiber anchoring/assembly protein TapA	-
BSF20_RS14615 (BSF20_14320)	sipW	2796419..2797003 (+)	585	WP_003153100.1	signal peptidase I SipW	-
BSF20_RS14620 (BSF20_14325)	tasA	2797067..2797852 (+)	786	WP_003153102.1	biofilm matrix protein TasA	-
BSF20_RS14625 (BSF20_14330)	sinR	2797900..2798235 (-)	336	WP_003153104.1	transcriptional regulator SinR	Regulator
BSF20_RS14630 (BSF20_14335)	sinI	2798269..2798442 (-)	174	WP_003153105.1	anti-repressor SinI	Regulator

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16312.84 Da
• Isoelectric Point: 10.3354

>NTDB_id=206548 BSF20_RS14580 WP_072588444.1 2793390..2793827(+) (comGD) [Bacillus amyloliquefaciens strain LM2303]
MNNNRRTENGFTLLESLIVLSLASVLLTVLFTTVPPVCTHLAVRQKTEQLQKDIQLAQETAIAEHKRTKITFLPKEHKYK
LQSSGRIVERSFDSLHITLVTLPESLEFNEKGHPNSGGKIRLKSAGFTYEITVYLGSGNVHAKRK

Nucleotide

• Length: 438 bp

>NTDB_id=206548 BSF20_RS14580 WP_072588444.1 2793390..2793827(+) (comGD) [Bacillus amyloliquefaciens strain LM2303]
TTGAACAATAACAGGCGGACAGAAAACGGGTTTACTCTTCTTGAAAGCCTGATTGTGTTAAGCCTGGCGTCCGTCCTGCT
GACGGTTTTGTTCACGACGGTTCCGCCGGTCTGCACCCACCTTGCCGTGCGGCAAAAAACAGAACAGCTTCAAAAAGATA
TTCAGCTTGCGCAGGAAACGGCTATCGCAGAACATAAAAGAACAAAAATCACTTTTCTTCCAAAAGAGCATAAATACAAA
CTGCAGTCAAGCGGTAGGATTGTCGAGCGTTCTTTTGATTCACTTCACATTACACTTGTGACTTTACCGGAGAGCCTTGA
ATTTAACGAAAAAGGCCATCCGAATTCGGGAGGAAAGATTCGATTGAAGAGCGCCGGGTTCACCTATGAGATCACAGTTT
ACTTAGGGAGCGGAAATGTCCATGCTAAACGGAAATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (5-31)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGD	Bacillus subtilis subsp. subtilis str. 168	55.479	100	0.559