Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PA0396 Genome accession   NC_002516
Coordinates   437782..438930 (+) Length   382 a.a.
NCBI ID   NP_249087.1    Uniprot ID   G3XCX3
Organism   Pseudomonas aeruginosa PAO1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 432782..443930
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PA0392 - 434226..434819 (-) 594 NP_249083.1 hypothetical protein -
  PA0393 proC 434830..435651 (-) 822 NP_249084.1 pyrroline-5-carboxylate reductase -
  PA0394 - 435663..436355 (-) 693 NP_249085.1 hypothetical protein -
  PA0395 pilT 436570..437604 (+) 1035 NP_249086.1 twitching motility protein PilT Machinery gene
  PA0396 pilU 437782..438930 (+) 1149 NP_249087.1 twitching motility protein PilU Machinery gene
  PA0397 - 438938..439837 (-) 900 NP_249088.1 cation efflux system protein -
  PA0398 - 439991..440395 (+) 405 NP_249089.1 hypothetical protein -
  PA0399 - 440638..442011 (+) 1374 NP_249090.1 cystathionine beta-synthase -
  PA0400 - 442008..443192 (+) 1185 NP_249091.1 cystathionine gamma-lyase -

Sequence

Protein


Download         Length: 382 a.a.        Molecular weight: 42532.81 Da        Isoelectric Point: 6.7792

>NTDB_id=20647 PA0396 NP_249087.1 437782..438930(+) (pilU) [Pseudomonas aeruginosa PAO1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide


Download         Length: 1149 bp        

>NTDB_id=20647 PA0396 NP_249087.1 437782..438930(+) (pilU) [Pseudomonas aeruginosa PAO1]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XCX3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

88.482

100

0.885
  pilU Acinetobacter baylyi ADP1

65.266

93.455

0.61
  pilU Vibrio cholerae strain A1552

60.286

91.623

0.552
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.068

88.743

0.382

Multiple sequence alignment