Detailed information NTG0020646

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: PA0395
• Genome accession: NC_002516
• Coordinates: 436570..437604 (+)
• Length: 344 a.a.
• NCBI ID: NP_249086.1
• Uniprot ID: P24559
• Organism: Pseudomonas aeruginosa PAO1
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 431570..442604

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PA0391	-	431858..433825 (-)	1968	NP_249082.1	hypothetical protein	-
PA0392	-	434226..434819 (-)	594	NP_249083.1	hypothetical protein	-
PA0393	proC	434830..435651 (-)	822	NP_249084.1	pyrroline-5-carboxylate reductase	-
PA0394	-	435663..436355 (-)	693	NP_249085.1	hypothetical protein	-
PA0395	pilT	436570..437604 (+)	1035	NP_249086.1	twitching motility protein PilT	Machinery gene
PA0396	pilU	437782..438930 (+)	1149	NP_249087.1	twitching motility protein PilU	Machinery gene
PA0397	-	438938..439837 (-)	900	NP_249088.1	cation efflux system protein	-
PA0398	-	439991..440395 (+)	405	NP_249089.1	hypothetical protein	-
PA0399	-	440638..442011 (+)	1374	NP_249090.1	cystathionine beta-synthase	-

Sequence

Protein

• Length: 344 a.a.
• Molecular weight: 38020.89 Da
• Isoelectric Point: 6.8364

>NTDB_id=20646 PA0395 NP_249086.1 436570..437604(+) (pilT) [Pseudomonas aeruginosa PAO1]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLEHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEELGMGEVFKRVSDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKAMVRSMLSESLQSVISQTLIKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KGLVAKGLISRENAREKAKIPENF

Nucleotide

• Length: 1035 bp

>NTDB_id=20646 PA0395 NP_249086.1 436570..437604(+) (pilT) [Pseudomonas aeruginosa PAO1]
ATGGATATTACCGAGCTGCTCGCCTTCAGTGCCAAACAGGGCGCTTCGGACCTGCACCTCTCCGCCGGCCTGCCACCCAT
GATCCGGGTGGATGGCGATGTACGCCGGATCAACCTGCCACCGCTGGAACACAAGCAGGTGCATGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGACTTCGAGGAATTCCTCGAGACCGACTTCTCCTTCGAGGTGCCGGGCGTCGCGCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGTGGCGCCGGCGCGGTATTCCGGACCATTCCCTCCAAGGTACTGACCATGGA
GGAGCTTGGCATGGGAGAAGTGTTCAAACGTGTTTCAGACGTCCCGCGCGGGTTGGTACTGGTCACCGGGCCGACCGGTT
CGGGCAAGTCCACCACCCTGGCGGCGATGCTCGATTACCTGAACAACACCAAGTACCACCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTCCACGAATCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCATCGCGACACCCTCGGCTTCAGCGA
AGCGCTGCGCTCGGCGCTGCGGGAGGACCCGGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CCCTGACCGCGGCGGAGACCGGCCACCTGGTATTCGGCACCCTGCACACCACCTCGGCGGCGAAGACCATCGACCGGGTG
GTCGACGTGTTCCCGGCCGAGGAAAAGGCCATGGTTCGCTCGATGCTCTCCGAGTCGCTGCAATCGGTGATCTCGCAGAC
CCTGATCAAGAAGATCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATCGGCACCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGGCGATCCAGACCGGCGGCTCGCTGGGCATGCAGACCCTCGACATGTGCCTC
AAGGGCCTGGTCGCCAAGGGCCTGATCAGCCGCGAGAACGCCCGCGAGAAGGCGAAGATCCCGGAAAACTTCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (11-271)

3D structure

• PDB: 3JVU
• PDB: 3JVV

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Pseudomonas aeruginosa PAK	100	100	1
pilT	Pseudomonas stutzeri DSM 10701	94.186	100	0.942
pilT	Acinetobacter baumannii D1279779	84.302	100	0.843
pilT	Acinetobacter baumannii strain A118	84.302	100	0.843
pilT	Acinetobacter nosocomialis M2	84.012	100	0.84
pilT	Acinetobacter baylyi ADP1	81.686	100	0.817
pilT	Legionella pneumophila strain Lp02	74.556	98.256	0.733
pilT	Legionella pneumophila strain ERS1305867	74.556	98.256	0.733
pilT	Neisseria meningitidis 8013	66.667	100	0.669
pilT	Neisseria gonorrhoeae MS11	66.377	100	0.666
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	66.765	98.837	0.66
pilT	Vibrio cholerae strain A1552	66.765	98.837	0.66
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	52.464	100	0.526
pilU	Vibrio cholerae strain A1552	41.298	98.547	0.407
pilU	Pseudomonas stutzeri DSM 10701	40.896	97.384	0.398
pilU	Acinetobacter baylyi ADP1	39.306	100	0.395