Detailed information NTG0205994

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BMJ37_RS12665
• Genome accession: NZ_CP018133
• Coordinates: 2593583..2594197 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain ATR2
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2588583..2599197

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BMJ37_RS12640 (BMJ37_12225)	rpsT	2588886..2589152 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BMJ37_RS12645 (BMJ37_12230)	holA	2589115..2590210 (-)	1096	Protein_2434	DNA polymerase III subunit delta	-
BMJ37_RS19905	-	2590250..2590401 (-)	152	Protein_2435	hypothetical protein	-
BMJ37_RS12650 (BMJ37_12235)	-	2590442..2590576 (+)	135	WP_003152870.1	YqzM family protein	-
BMJ37_RS12655 (BMJ37_12240)	comEC	2590595..2592946 (-)	2352	WP_029973403.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BMJ37_RS12660 (BMJ37_12245)	-	2592947..2593516 (-)	570	WP_003152868.1	ComE operon protein 2	-
BMJ37_RS12665 (BMJ37_12250)	comEA	2593583..2594197 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
BMJ37_RS12670 (BMJ37_12255)	comER	2594256..2595077 (+)	822	WP_029973401.1	late competence protein ComER	-
BMJ37_RS12675 (BMJ37_12260)	-	2595146..2595883 (-)	738	WP_015417856.1	class I SAM-dependent DNA methyltransferase	-
BMJ37_RS12680 (BMJ37_12265)	rsfS	2595880..2596236 (-)	357	WP_007408260.1	ribosome silencing factor	-
BMJ37_RS12685 (BMJ37_12270)	yqeK	2596254..2596814 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BMJ37_RS12690 (BMJ37_12275)	-	2596804..2597373 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
BMJ37_RS12695 (BMJ37_12280)	yhbY	2597384..2597674 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BMJ37_RS12700 (BMJ37_12285)	aroE	2597668..2598510 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=205994 BMJ37_RS12665 WP_014418419.1 2593583..2594197(-) (comEA) [Bacillus velezensis strain ATR2]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=205994 BMJ37_RS12665 WP_014418419.1 2593583..2594197(-) (comEA) [Bacillus velezensis strain ATR2]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363