Detailed information NTG0020587

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: XF_RS06915
• Genome accession: NC_002488
• Coordinates: 1571071..1572201 (-)
• Length: 376 a.a.
• NCBI ID: WP_010894128.1
• Uniprot ID: Q9PCX2
• Organism: Xylella fastidiosa 9a5c
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1566071..1577201

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
XF_RS06895 (XF1626)	-	1566142..1566900 (+)	759	WP_010894122.1	LytR/AlgR family response regulator transcription factor	-
XF_RS06900 (XF1627)	hemC	1566917..1567834 (+)	918	WP_010894123.1	hydroxymethylbilane synthase	-
XF_RS06905 (XF1628)	-	1567888..1568697 (-)	810	WP_031336664.1	DUF481 domain-containing protein	-
XF_RS06910 (XF1630)	-	1569541..1570554 (+)	1014	WP_229716473.1	helix-turn-helix transcriptional regulator	-
XF_RS06915 (XF1632)	pilU	1571071..1572201 (-)	1131	WP_010894128.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
XF_RS06920 (XF1633)	pilT	1572367..1573401 (-)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
XF_RS14660 (XF1634)	-	1573470..1573667 (-)	198	WP_010894129.1	hypothetical protein	-
XF_RS06935 (XF1637)	-	1574664..1575491 (+)	828	WP_173405994.1	glycosyltransferase family 2 protein	-
XF_RS06940 (XF1638)	-	1576155..1576979 (-)	825	WP_010894133.1	glycosyltransferase family 2 protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42476.67 Da
• Isoelectric Point: 6.5027

>NTDB_id=20587 XF_RS06915 WP_010894128.1 1571071..1572201(-) (pilU) [Xylella fastidiosa 9a5c]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITN
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLTDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=20587 XF_RS06915 WP_010894128.1 1571071..1572201(-) (pilU) [Xylella fastidiosa 9a5c]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAAGTCCATGAATGCAACTTCGCAATTGACATCACCAAC
ATTGGTCGCTTCCGCGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACTCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCAGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGACTGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACTACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCCAAAACTCTGAGTCAAGGATTCGATGGAGTAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-272)

3D structure

• AlphaFold DB: Q9PCX2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.58	97.606	0.601
pilU	Acinetobacter baylyi ADP1	59.887	94.149	0.564
pilU	Vibrio cholerae strain A1552	51.124	94.681	0.484
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.209	96.809	0.399