Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BMF22_RS05990 Genome accession   NZ_CP017961
Coordinates   1240678..1242060 (+) Length   460 a.a.
NCBI ID   WP_005751075.1    Uniprot ID   -
Organism   Pasteurella multocida strain Razi_Pm0001     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1235678..1247060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BMF22_RS05960 (BMF22_05940) - 1235870..1236721 (-) 852 WP_005751072.1 thymidylate synthase -
  BMF22_RS05965 (BMF22_05945) lgt 1236727..1237530 (-) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  BMF22_RS05970 (BMF22_05950) - 1237540..1238334 (-) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  BMF22_RS05975 (BMF22_05955) rppH 1238337..1238933 (-) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  BMF22_RS05980 (BMF22_05960) ampD 1239519..1240085 (-) 567 WP_005751074.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BMF22_RS05985 (BMF22_05965) ppdD 1240245..1240678 (+) 434 Protein_1167 prepilin peptidase-dependent pilin -
  BMF22_RS05990 (BMF22_05970) pilB 1240678..1242060 (+) 1383 WP_005751075.1 GspE/PulE family protein Machinery gene
  BMF22_RS05995 (BMF22_05975) - 1242060..1243270 (+) 1211 Protein_1169 type II secretion system F family protein -
  BMF22_RS06000 (BMF22_05980) - 1243270..1243992 (+) 723 WP_005727302.1 prepilin peptidase -
  BMF22_RS06005 (BMF22_05985) coaE 1244007..1244627 (+) 621 WP_005751078.1 dephospho-CoA kinase -
  BMF22_RS06010 (BMF22_05990) yacG 1244638..1244841 (+) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  BMF22_RS06015 (BMF22_05995) - 1244841..1245110 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  BMF22_RS06020 (BMF22_06000) - 1245559..1246932 (+) 1374 WP_005727312.1 alanine/glycine:cation symporter family protein -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 52017.66 Da        Isoelectric Point: 6.5002

>NTDB_id=204482 BMF22_RS05990 WP_005751075.1 1240678..1242060(+) (pilB) [Pasteurella multocida strain Razi_Pm0001]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLST
NELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQV
QPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGMT
ADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRTF
LRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCDCRVE
TQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=204482 BMF22_RS05990 WP_005751075.1 1240678..1242060(+) (pilB) [Pasteurella multocida strain Razi_Pm0001]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGCGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACGCTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGTGTGG
ATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCAACA
AATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCAAGT
CGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTACAGC
TACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAAGTC
CAGCCTTCCTTTTCAGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGAAAC
ACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGCCGA
CGCATTTAGGCGAAAAAGCGGTATTACGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATGACC
GCAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAGCCTCAAGGACTGATTTTAGTAACGGGACCAACAGGGAGTGG
AAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGATTG
AAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACTTTT
TTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGCTCA
AACAGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGGTGC
CTTTGCATGAAATTGAACAAAGCCTCTTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTGATTGCCGTGTAGAG
ACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAACAGG
GTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATGAAG
TACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(127-393)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(18-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.991

98.478

0.67
  pilB Haemophilus influenzae Rd KW20

67.329

98.478

0.663
  pilB Glaesserella parasuis strain SC1401

58.811

98.696

0.58
  pilB Acinetobacter baumannii D1279779

40.369

100

0.428
  pilB Vibrio parahaemolyticus RIMD 2210633

39.059

100

0.415
  pilB Vibrio campbellii strain DS40M4

39.419

100

0.413
  pilB Acinetobacter baylyi ADP1

37.876

100

0.411
  pilB Vibrio cholerae strain A1552

38.272

100

0.404
  pilB Legionella pneumophila strain ERS1305867

37.243

100

0.393

Multiple sequence alignment