Detailed information NTG0202567

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: BJN34_RS17500
• Genome accession: NZ_CP017757
• Coordinates: 3780750..3782474 (+)
• Length: 574 a.a.
• NCBI ID: WP_078197961.1
• Uniprot ID: A0A1U9USJ8
• Organism: Cupriavidus necator strain NH9
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3764110..3780420	3780750..3782474	flank	330

Gene organization within MGE regions

Location: 3764110..3782474

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BJN34_RS17405 (BJN34_17370)	-	3764110..3765396 (+)	1287	WP_078197943.1	hypothetical protein	-
BJN34_RS17415 (BJN34_17375)	-	3766027..3767073 (-)	1047	WP_164704875.1	PD-(D/E)XK motif protein	-
BJN34_RS17425 (BJN34_17380)	-	3768039..3768854 (-)	816	WP_078197947.1	phage/plasmid primase, P4 family	-
BJN34_RS17430 (BJN34_17385)	-	3768851..3770116 (-)	1266	WP_078197948.1	IS256 family transposase	-
BJN34_RS17435 (BJN34_17390)	-	3770229..3770735 (-)	507	WP_078197949.1	hypothetical protein	-
BJN34_RS17440 (BJN34_17395)	-	3770728..3770910 (-)	183	WP_078197950.1	helix-turn-helix domain-containing protein	-
BJN34_RS17445 (BJN34_17400)	-	3771459..3771899 (+)	441	WP_078197951.1	hypothetical protein	-
BJN34_RS17455 (BJN34_17405)	-	3772794..3773129 (-)	336	WP_078197952.1	hypothetical protein	-
BJN34_RS17460 (BJN34_17410)	-	3773172..3773603 (-)	432	WP_078197953.1	hypothetical protein	-
BJN34_RS17470 (BJN34_17415)	-	3774333..3775481 (-)	1149	WP_078197955.1	hypothetical protein	-
BJN34_RS17475 (BJN34_17420)	-	3775875..3777746 (-)	1872	WP_078197956.1	DUF262 domain-containing protein	-
BJN34_RS17480 (BJN34_17425)	-	3777965..3778426 (-)	462	WP_078197957.1	helix-turn-helix domain-containing protein	-
BJN34_RS17485 (BJN34_17430)	-	3778656..3779045 (-)	390	WP_078197958.1	hypothetical protein	-
BJN34_RS17490 (BJN34_17435)	-	3779777..3780088 (-)	312	WP_234824849.1	helix-turn-helix domain-containing protein	-
BJN34_RS17495 (BJN34_17440)	-	3780094..3780420 (-)	327	WP_078197960.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BJN34_RS17500 (BJN34_17445)	pilF	3780750..3782474 (+)	1725	WP_078197961.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 574 a.a.
• Molecular weight: 63394.11 Da
• Isoelectric Point: 7.0954

>NTDB_id=202567 BJN34_RS17500 WP_078197961.1 3780750..3782474(+) (pilF) [Cupriavidus necator strain NH9]
MTLGLALAQSRRIAPALLAQLEQAAREKQSQLIDEIVGSGTMSAHDLALFAADKYQLPLLDLAQYNLTKVPPALAGNREF
HAHRLLPLGRRENRLLLAMSDPSNQAGLDAIKEKYKLPVEAVVVEHDKLMKHVRSAGEALGTMKNISPVQAERKMIEYDP
VAAAGNQRNRTTADNIDDAPVVRFLQKLLTEAFHRGASDLHFEPFETFYRVRFRVDGVLQEVARPPLDIRDKIATRIKVL
SRLDISEKRVPQDGRMKLLLALPKDKDAKETVERAVDFRVSTLPTLFGEKIVMRILESSSDKLDIDQLGYEPEQKALLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNLLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALRSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAAFNIASSVLMITAQRLARRLCTCKRQGELPHEALL
EAGFREQDLDGSWQPYHPVGCERCNGTGYKGRCGIYQVMPITEAMQEIILTHGTALQIAEQARKDGVLSLREAGLLKVKQ
GVTSLEEVLATTNI

Nucleotide

• Length: 1725 bp

>NTDB_id=202567 BJN34_RS17500 WP_078197961.1 3780750..3782474(+) (pilF) [Cupriavidus necator strain NH9]
ATGACACTCGGTCTTGCCCTGGCCCAGAGCCGGCGTATCGCGCCCGCCCTGCTTGCTCAGCTGGAGCAGGCCGCGCGTGA
AAAGCAATCGCAGCTGATCGACGAGATCGTCGGCAGCGGCACCATGAGCGCGCACGACCTGGCGCTGTTCGCCGCGGACA
AGTACCAGCTGCCGCTGCTGGACCTGGCCCAGTACAACCTGACCAAGGTGCCGCCGGCGCTGGCCGGCAACCGCGAGTTC
CACGCGCACCGGCTGCTGCCGCTGGGCCGGCGCGAGAACCGGCTGTTGCTGGCGATGTCCGACCCGTCCAACCAGGCCGG
GCTGGATGCCATCAAGGAAAAGTACAAGCTGCCAGTCGAAGCGGTGGTGGTCGAGCACGACAAGCTGATGAAGCATGTGC
GCTCCGCTGGCGAGGCCCTGGGCACGATGAAGAACATCTCGCCGGTGCAGGCCGAGCGCAAGATGATCGAATACGATCCG
GTGGCCGCGGCCGGCAACCAGCGCAACCGCACCACCGCCGACAATATCGACGACGCCCCGGTGGTGCGTTTCCTGCAGAA
GCTGCTGACCGAGGCCTTCCACCGCGGCGCCTCGGACCTGCACTTCGAGCCGTTCGAGACCTTCTACCGCGTGCGCTTCC
GTGTCGACGGCGTGCTGCAGGAGGTCGCCCGGCCGCCGCTGGATATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTG
TCGCGGCTGGATATTTCTGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGTTGCTGCTCGCCCTGCCCAAGGACAAGGA
CGCCAAGGAGACGGTCGAGCGGGCGGTGGACTTCCGCGTATCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATGC
GGATTCTCGAATCGTCGTCCGACAAGCTCGATATCGACCAGCTCGGCTATGAGCCCGAGCAGAAGGCGCTGCTGCTGGAC
GTGATCAAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCCACCGGCAGCGGCAAGACGGTATCCCTGTACACCTTCCT
GAACCTGCTGAACCAGGGCGACATCAATATCTCCACGGCGGAGGACCCGGCCGAAATCCAGCTGCCGGGCATCAACCAGG
TCAACGTCAACGACAAGGCGGGCCTGACCTTTGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCGGACATCATCATG
GTCGGCGAAATCCGCGACCTGGAAACCGCCGATATCTCGATCAAGGCCGCGCAGACCGGCCACCTGGTGTTGTCGACGCT
GCACACCAACGATGCGCCGACCACGCTGACCCGCCTGATGAACATGGGCGTGGCGGCCTTCAATATTGCGTCGAGCGTCC
TGATGATCACCGCGCAGCGGCTGGCGCGGCGGCTGTGCACCTGCAAGCGCCAGGGCGAGCTTCCGCACGAGGCGCTGCTG
GAGGCGGGTTTCCGCGAGCAAGACCTGGACGGCAGCTGGCAGCCCTACCACCCGGTCGGCTGCGAGCGCTGCAATGGCAC
CGGCTACAAGGGCCGCTGCGGCATCTACCAGGTCATGCCGATCACCGAGGCCATGCAGGAGATCATCCTGACGCACGGCA
CCGCCTTGCAGATTGCCGAGCAGGCGCGCAAGGACGGCGTGCTATCGTTGCGCGAAGCGGGGCTGCTGAAGGTGAAGCAG
GGCGTCACGTCACTCGAAGAAGTGCTGGCGACTACAAATATTTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (187-464)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (40-134)

3D structure

• AlphaFold DB: A0A1U9USJ8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Neisseria gonorrhoeae MS11	51.468	100	0.519
pilB	Acinetobacter baumannii D1279779	51.916	100	0.519
pilB	Acinetobacter baylyi ADP1	52.252	96.69	0.505
pilB	Legionella pneumophila strain ERS1305867	50.091	95.993	0.481
pilB	Vibrio cholerae strain A1552	44.894	98.955	0.444
pilB	Vibrio campbellii strain DS40M4	46.296	94.077	0.436
pilB	Vibrio parahaemolyticus RIMD 2210633	46.283	93.728	0.434
pilF	Thermus thermophilus HB27	38.821	100	0.39
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.421	96.341	0.38