Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   PSJM300_02705 Genome accession   CP003725
Coordinates   600846..601910 (+) Length   354 a.a.
NCBI ID   AFN76619.1    Uniprot ID   -
Organism   Pseudomonas stutzeri DSM 10701     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 595846..606910
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSJM300_02690 - 595898..596698 (+) 801 AFN76616.1 nuclease -
  PSJM300_02695 - 596843..598111 (+) 1269 AFN76617.1 malic enzyme -
  PSJM300_02700 - 598234..600666 (-) 2433 AFN76618.1 penicillin-binding protein 1A -
  PSJM300_02705 comM 600846..601910 (+) 1065 AFN76619.1 type IV pilus assembly protein PilM Machinery gene
  PSJM300_02710 - 601910..602482 (+) 573 AFN76620.1 type 4 fimbrial biogenesis protein PilN -
  PSJM300_02715 - 602479..603102 (+) 624 AFN76621.1 type 4 fimbrial biogenesis protein PilO -
  PSJM300_02720 - 603099..603626 (+) 528 AFN76622.1 type 4 fimbrial biogenesis protein PilP -
  PSJM300_02725 pilQ 603680..605785 (+) 2106 AFN76623.1 type 4 fimbrial biogenesis protein PilQ Machinery gene
  PSJM300_02730 - 605846..606334 (+) 489 AFN76624.1 shikimate kinase -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37855.24 Da        Isoelectric Point: 4.6355

>NTDB_id=20255 PSJM300_02705 AFN76619.1 600846..601910(+) (comM) [Pseudomonas stutzeri DSM 10701]
MLGLFTKKANTLLGIDISSTSVKLLELSRTGNRYRVEAYAVEPLPANAVVEKNIAELEGVGQALSRLLSKAKSGVKSAAV
AVSGSAVITKSIEMDAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGPAARSPGRVEVLLAACRKENVEIREAALAL
AGLTAKVVDVEAYALERSYSLLAPQLGAGHDELTVALVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSM
EEAGLAKKQGGLPDDYESEVLQPFKEAVVQQVSRSLQFFFAAGQFHDVDYILLAGGTASIPNLDRLIQQKIGTQTLVANP
FADMALSGKVNAGALASDAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=20255 PSJM300_02705 AFN76619.1 600846..601910(+) (comM) [Pseudomonas stutzeri DSM 10701]
GTGCTAGGGCTCTTCACTAAGAAAGCGAATACGCTGCTCGGGATCGATATCAGTTCGACGTCCGTCAAGCTCCTGGAATT
AAGTCGCACCGGCAATCGGTATCGGGTCGAAGCCTATGCTGTCGAGCCGCTGCCGGCGAATGCCGTTGTCGAAAAGAACA
TCGCCGAGCTTGAGGGTGTCGGCCAGGCCTTGTCGCGTCTGTTGAGCAAGGCCAAAAGCGGCGTCAAGTCCGCGGCGGTC
GCGGTTTCCGGCTCGGCGGTCATCACCAAGAGCATCGAGATGGATGCCGGCCTTTCCGACGACGAGTTGGAAAACCAGCT
CAAGATCGAAGCCGACCAGTACATTCCTTATCCGCTTGAAGAGGTCGCGATCGACTTCGAAGTGCAGGGCCCTGCCGCAC
GATCGCCCGGGCGAGTGGAAGTGTTGCTCGCCGCTTGCCGGAAGGAAAACGTCGAGATCCGCGAGGCCGCGCTGGCTCTG
GCCGGCCTGACTGCCAAAGTCGTGGATGTCGAGGCTTACGCTCTGGAGCGTTCCTACAGCCTGCTGGCGCCCCAGCTGGG
GGCGGGCCACGATGAGTTGACCGTTGCGCTGGTTGACATTGGGGCGACGATGACCACCCTGAGCGTCCTGCACAACGGTC
GCACCATCTATACGCGTGAGCAATTGTTCGGCGGACGCCAGTTGACGGAAGAAATCCAGCGGCGTTACGGACTTTCCATG
GAAGAGGCCGGCCTTGCCAAGAAGCAGGGCGGCTTGCCGGACGATTACGAAAGCGAAGTGCTGCAGCCGTTCAAGGAGGC
CGTCGTTCAGCAGGTCTCGCGTTCGCTGCAGTTCTTCTTCGCTGCCGGGCAGTTTCACGATGTCGATTACATCCTGCTCG
CTGGCGGCACCGCGTCGATTCCCAACCTCGATCGACTGATCCAGCAGAAGATCGGTACCCAGACGCTGGTTGCCAATCCA
TTCGCCGACATGGCCCTGAGCGGCAAGGTCAACGCAGGCGCGCTGGCCAGTGATGCGCCTTCCCTGATGATCGCCTGCGG
ACTGGCGCTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

57.345

100

0.573
  pilM Acinetobacter baumannii D1279779

57.345

100

0.573
  comM Acinetobacter baylyi ADP1

54.802

100

0.548
  pilM Legionella pneumophila strain ERS1305867

47.74

100

0.477

Multiple sequence alignment