Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PSJM300_02725 Genome accession   CP003725
Coordinates   603680..605785 (+) Length   701 a.a.
NCBI ID   AFN76623.1    Uniprot ID   -
Organism   Pseudomonas stutzeri DSM 10701     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 598680..610785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSJM300_02705 comM 600846..601910 (+) 1065 AFN76619.1 type IV pilus assembly protein PilM Machinery gene
  PSJM300_02710 - 601910..602482 (+) 573 AFN76620.1 type 4 fimbrial biogenesis protein PilN -
  PSJM300_02715 - 602479..603102 (+) 624 AFN76621.1 type 4 fimbrial biogenesis protein PilO -
  PSJM300_02720 - 603099..603626 (+) 528 AFN76622.1 type 4 fimbrial biogenesis protein PilP -
  PSJM300_02725 pilQ 603680..605785 (+) 2106 AFN76623.1 type 4 fimbrial biogenesis protein PilQ Machinery gene
  PSJM300_02730 - 605846..606334 (+) 489 AFN76624.1 shikimate kinase -
  PSJM300_02735 aroB 606358..607458 (+) 1101 AFN76625.1 3-dehydroquinate synthase -
  PSJM300_02740 - 607468..608988 (+) 1521 AFN76626.1 sporulation domain-containing protein -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75311.33 Da        Isoelectric Point: 4.6879

>NTDB_id=20256 PSJM300_02725 AFN76623.1 603680..605785(+) (pilQ) [Pseudomonas stutzeri DSM 10701]
MNSTLSRLGLSLMAALVSPALLAANLNALDVASLPGDRVELKLAFDEPVAAPRGYTLDQPARIALDLPGVSSKLDSKNRE
LGVGNARSVTVVEAQGRTRLIVNLTTLVPYSTRVDGNNLFVVLGESGAAAPAAVGPVAAPVVQPTPAAVQSKSISNIDFR
RGDDGAGNVVISLSDPSISPNIEEQGGKIRVTFAKTQLPEALRVRLDVQDFATPVKFVDATSQGGAASIAIEPSGRYDYL
AYQTDDKLTISIKPLTADEAEKRNAEHFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNVPWDQ
ALALVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIAELAPLRREVVQVNYAKAADIARLFQSVTSNGASTEDR
GSIAVDDRTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKALGVRWGGNYVNDQWSAYGKDGSRSVD
DDGFVRLPGTDTVGGFKLDEGVAPVPFVDMGVLGSTSGVGIGFISDHVMLDLQLSAMEKTGNGEVVSQPKVMTADKETAK
ILKGTEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFRNAMNGIPPIDKNEVNAKILVSDGET
VVIGGVFSNSQSKSVDKVPFLGDLPFLGRLFRRDVVTDAKSELLVFLTPRILNNQAIAVNR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=20256 PSJM300_02725 AFN76623.1 603680..605785(+) (pilQ) [Pseudomonas stutzeri DSM 10701]
ATGAACAGCACCCTTTCTCGCCTCGGCCTTTCCCTGATGGCGGCCCTTGTTTCACCTGCTTTGCTGGCTGCCAACCTCAA
CGCCCTGGACGTTGCCTCGCTGCCCGGGGATCGGGTGGAGCTGAAGTTGGCTTTCGACGAGCCCGTCGCTGCACCGCGCG
GCTATACGCTCGATCAGCCGGCGCGCATCGCACTGGATCTTCCCGGTGTCTCCAGCAAGCTGGATTCGAAGAACCGTGAG
CTGGGCGTCGGTAATGCGCGCAGTGTAACGGTGGTGGAAGCTCAAGGGCGGACTCGTCTGATCGTCAACCTCACCACGCT
GGTGCCTTATTCGACCCGTGTCGATGGCAACAACCTTTTCGTGGTGCTGGGTGAAAGCGGCGCAGCCGCTCCGGCAGCCG
TTGGTCCTGTAGCCGCTCCGGTCGTCCAGCCGACTCCGGCGGCGGTGCAGAGCAAGTCCATCAGCAACATCGATTTTCGC
CGTGGGGACGATGGAGCAGGCAACGTCGTCATCTCCTTGTCGGATCCATCGATCAGCCCGAACATCGAAGAGCAGGGTGG
CAAGATTCGCGTCACCTTCGCCAAGACACAATTGCCGGAAGCCCTGCGCGTTCGGCTGGATGTGCAGGATTTCGCTACCC
CGGTCAAGTTCGTCGATGCGACCAGTCAAGGAGGTGCCGCGAGCATCGCCATCGAGCCGAGTGGTCGCTATGACTACCTC
GCGTATCAGACGGATGACAAGCTCACCATCAGCATCAAGCCGCTGACCGCGGATGAAGCGGAGAAGCGCAACGCAGAACA
TTTCGCCTATTCCGGTGAGAAGCTCTCGCTCAACTTTCAGGACATCGACGTCCGCTCGGTGTTGCAGCTGATCGCGGACT
TTACCGATCTGAACCTGGTCGCCAGTGATACGGTACAGGGCAATATCACGCTGCGTCTGCAGAACGTGCCTTGGGACCAG
GCCTTGGCACTGGTGCTCAAGACCAAGGGATTGGACAAGCGTCAGGTCGGCAACGTACTGCTGGTTGCGCCGGCCGACGA
GATCGCCGCACGTGAGCGTCAGGAGCTGGAATCCCAGCGCCAGATCGCCGAATTGGCGCCGCTGCGTCGTGAAGTAGTGC
AGGTCAACTATGCCAAGGCCGCCGACATTGCACGTCTGTTCCAGTCGGTGACCAGCAATGGGGCCTCGACAGAAGATCGC
GGCTCGATAGCCGTCGATGATCGCACCAACAACATCATCGCCTATCAGACACAGGAACGACTGGATGAACTGCGTCGCAT
CGTGGCGCAGCTGGACATTCCGGTACGTCAGGTGATGATCGAGGCGCGGATCGTCGAGGCGAACGTGGACTACGATAAGG
CGCTTGGGGTTCGTTGGGGTGGGAACTATGTCAATGATCAATGGAGTGCCTACGGAAAGGATGGCAGTCGCTCGGTCGAT
GATGATGGCTTCGTTAGGCTACCTGGCACCGATACAGTCGGTGGATTCAAGTTGGACGAAGGTGTAGCCCCGGTGCCATT
CGTTGATATGGGCGTCTTGGGTAGTACGTCTGGCGTTGGGATCGGTTTTATCTCTGATCATGTCATGTTGGATCTTCAAC
TTTCGGCAATGGAGAAAACCGGAAACGGAGAGGTGGTATCCCAGCCGAAGGTCATGACTGCAGACAAAGAAACAGCCAAG
ATTCTGAAGGGCACAGAAGTGCCTTACCAGGAAGCTAGCTCCAGCGGCGCGACCAGCACATCGTTCAAAGAGGCGGCCTT
GTCTCTCGAGGTCACTCCTCAAATTACACCGGACAACCGCATCATCATGGAAGTGAAGGTCACTAAAGATGCCCCTGATT
TCCGTAATGCAATGAACGGTATTCCCCCGATTGACAAGAACGAAGTCAATGCCAAAATTTTGGTGAGCGATGGAGAAACT
GTAGTGATTGGTGGTGTATTCTCGAACAGTCAATCCAAGAGCGTCGACAAGGTGCCGTTCTTGGGTGACTTACCATTCCT
GGGGCGGCTGTTCCGTCGTGATGTCGTGACTGATGCCAAATCTGAGCTACTGGTATTCCTGACCCCTAGAATTCTGAACA
ACCAGGCTATCGCAGTGAATCGTTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(298-344)

Bacterial type II/III secretion system short domain (Secretin_N)

(371-437)

Bacterial type II and III secretion system protein (Secretin)

(536-692)

AMIN domain (AMIN)

(38-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

74.51

100

0.759
  pilQ Acinetobacter baumannii strain A118

39.891

100

0.419
  pilQ Acinetobacter baumannii D1279779

39.891

100

0.419
  comQ Acinetobacter baylyi ADP1

41.143

99.857

0.411
  pilQ Legionella pneumophila strain Lp02

36.865

98.288

0.362
  pilQ Legionella pneumophila strain ERS1305867

36.865

98.288

0.362

Multiple sequence alignment