Detailed information NTG0202205

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BKP64_RS11965
• Genome accession: NZ_CP017715
• Coordinates: 2578266..2579330 (-)
• Length: 354 a.a.
• NCBI ID: WP_070970294.1
• Uniprot ID: A0A1D9GMG4
• Organism: Marinobacter salinus strain Hb8
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2573266..2584330

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BKP64_RS11940 (BKP64_11940)	aroK	2573706..2574272 (-)	567	WP_070970282.1	shikimate kinase AroK	-
BKP64_RS11945 (BKP64_11945)	pilQ	2574409..2576475 (-)	2067	WP_227515381.1	type IV pilus secretin PilQ family protein	Machinery gene
BKP64_RS11950 (BKP64_11950)	-	2576541..2577089 (-)	549	WP_070970286.1	pilus assembly protein PilP	-
BKP64_RS11955 (BKP64_11955)	-	2577092..2577703 (-)	612	WP_070970289.1	type 4a pilus biogenesis protein PilO	-
BKP64_RS11960 (BKP64_11960)	-	2577703..2578266 (-)	564	WP_070970292.1	PilN domain-containing protein	-
BKP64_RS11965 (BKP64_11965)	pilM	2578266..2579330 (-)	1065	WP_070970294.1	pilus assembly protein PilM	Machinery gene
BKP64_RS11970 (BKP64_11970)	-	2579526..2581988 (+)	2463	WP_070970298.1	penicillin-binding protein 1A	-
BKP64_RS11975 (BKP64_11975)	-	2582067..2583332 (-)	1266	WP_070970301.1	malic enzyme-like NAD(P)-binding protein	-
BKP64_RS11980 (BKP64_11980)	rpmE	2583549..2583770 (-)	222	WP_070970304.1	50S ribosomal protein L31	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38004.09 Da
• Isoelectric Point: 4.1788

>NTDB_id=202205 BKP64_RS11965 WP_070970294.1 2578266..2579330(-) (pilM) [Marinobacter salinus strain Hb8]
MFGLFGKKSSAVLGVDVSSSSVKLLELSKQGDRYKVESYAVEPLPANAVVEKNITDVEAVGEVLKRVAAKSRASVKQVAV
AVSGSAVITKLIQMDGGLNEFEMEDQITLEADQYIPYPLDEVAIDFEVQGPSENNPDQVDVLLAACRKENVDIREDALEI
ASLSAKIVDVEAYALERAYKLIEPQLDSQGESLVVAIVDVGATMTTLSVLAEGKTVYTREQIFGGKQLTEEIQRRYGLSL
EEAGLAKKQGGLPDDYESEVLTPFREAVVQQVARALQFFFGASQYNAVDYVVLAGGTASIQGLTEMVEEKTGTPTLVANP
FADMAVGSRVNASALSNDAPSLMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=202205 BKP64_RS11965 WP_070970294.1 2578266..2579330(-) (pilM) [Marinobacter salinus strain Hb8]
GTGTTCGGATTGTTCGGAAAGAAATCCAGTGCAGTGCTGGGCGTGGATGTAAGCTCCAGCTCAGTGAAACTTCTGGAGCT
GTCGAAGCAGGGCGACCGGTATAAGGTCGAGAGCTACGCAGTTGAGCCGTTGCCAGCAAATGCTGTCGTCGAGAAAAATA
TCACGGATGTCGAGGCCGTGGGTGAGGTGCTTAAGCGCGTTGCCGCCAAGTCCCGTGCCAGTGTGAAACAGGTGGCGGTA
GCGGTCTCCGGGTCGGCGGTTATTACCAAGCTTATCCAGATGGATGGCGGGCTTAACGAATTTGAAATGGAAGACCAGAT
CACGCTTGAGGCTGATCAGTACATTCCATATCCCCTCGATGAAGTCGCCATCGACTTTGAGGTGCAAGGGCCTTCCGAAA
ACAATCCGGATCAGGTTGATGTTCTTTTGGCTGCCTGCCGAAAGGAGAACGTCGATATTCGAGAGGATGCTCTGGAAATT
GCATCACTTTCGGCGAAGATCGTTGATGTGGAAGCTTACGCGCTTGAGCGCGCCTACAAGCTCATTGAACCTCAGCTCGA
TTCCCAGGGAGAGTCTCTCGTCGTCGCCATCGTTGATGTTGGTGCAACGATGACTACGCTCAGCGTCCTTGCCGAAGGGA
AGACCGTTTACACGCGCGAACAGATTTTCGGCGGCAAGCAGTTGACCGAGGAAATTCAGCGACGCTACGGGCTCTCTCTT
GAAGAGGCCGGCCTTGCCAAAAAACAGGGAGGCCTTCCGGATGATTATGAATCCGAGGTGCTCACGCCCTTCCGGGAGGC
TGTGGTTCAACAGGTCGCCCGGGCTCTCCAGTTCTTTTTTGGTGCCAGTCAGTACAACGCTGTCGACTATGTGGTTTTGG
CAGGTGGTACTGCCTCGATACAGGGTCTGACAGAAATGGTCGAAGAAAAAACCGGTACGCCCACTCTGGTTGCGAACCCG
TTTGCCGATATGGCGGTGGGGTCAAGGGTCAACGCATCAGCTTTGAGTAACGATGCTCCCTCACTCATGATTGCATGTGG
CCTGGCAATGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A1D9GMG4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	53.39	100	0.534
comM	Acinetobacter nosocomialis M2	53.39	100	0.534
comM	Acinetobacter baylyi ADP1	50.565	100	0.506
pilM	Legionella pneumophila strain ERS1305867	44.915	100	0.449