Detailed information NTG0202196

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: BKP64_RS04960
• Genome accession: NZ_CP017715
• Coordinates: 1082642..1083799 (-)
• Length: 385 a.a.
• NCBI ID: WP_070966761.1
• Uniprot ID: A0A1D9GJ44
• Organism: Marinobacter salinus strain Hb8
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1077642..1088799

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BKP64_RS04940 (BKP64_04940)	-	1078100..1079322 (+)	1223	Protein_972	patatin-like phospholipase family protein	-
BKP64_RS04945 (BKP64_04945)	prmB	1079420..1080322 (+)	903	WP_070966756.1	50S ribosomal protein L3 N(5)-glutamine methyltransferase	-
BKP64_RS04950 (BKP64_04950)	aroC	1080360..1081454 (+)	1095	WP_070966758.1	chorismate synthase	-
BKP64_RS04955 (BKP64_04955)	-	1081473..1082678 (+)	1206	WP_198402646.1	MFS transporter	-
BKP64_RS04960 (BKP64_04960)	pilU	1082642..1083799 (-)	1158	WP_070966761.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
BKP64_RS04965 (BKP64_04965)	-	1083830..1084705 (-)	876	WP_070966764.1	LysR family transcriptional regulator	-
BKP64_RS04970 (BKP64_04970)	leuC	1084882..1086300 (+)	1419	WP_070966767.1	3-isopropylmalate dehydratase large subunit	-
BKP64_RS04975 (BKP64_04975)	leuD	1086318..1086965 (+)	648	WP_070966770.1	3-isopropylmalate dehydratase small subunit	-
BKP64_RS04980 (BKP64_04980)	leuB	1087004..1088077 (+)	1074	WP_070966772.1	3-isopropylmalate dehydrogenase	-

Sequence

Protein

• Length: 385 a.a.
• Molecular weight: 42498.51 Da
• Isoelectric Point: 5.8344

>NTDB_id=202196 BKP64_RS04960 WP_070966761.1 1082642..1083799(-) (pilU) [Marinobacter salinus strain Hb8]
MGFDDYLRILAKHDGSDLYLSTGAPPCAKFHGSLKPIDRTPLAPGIIKEIAYSIMDDEQVSEFEQELEMNLAYSIRSVGR
FRINIFRQRNEISIVARNIVTDIPNADDLGLPSILKDVVMAKRGLVLFVGATGSGKSTSLAALIDHRNSNSAGHIITIED
PVEYIHRHKKSIVNQREVGVDTRSFQKALKNTLRQAPDVILIGEIRDRETMEHALAFAETGHLCISTLHANNANQALDRI
INFFPEERRAQLLMDLAMNLRAFISQRLIPTVDKKRCAAIEILLGTPIISELILRGEFDGIKEIMEKSANLGMQTFDLAL
FNLWKDGKISEEEALKNADSANNLRLKIKLHGQGGAGSTSSPKSGAPSGSGLSLQMEDDEDDSPE

Nucleotide

• Length: 1158 bp

>NTDB_id=202196 BKP64_RS04960 WP_070966761.1 1082642..1083799(-) (pilU) [Marinobacter salinus strain Hb8]
ATGGGGTTTGATGACTACTTACGAATTCTGGCCAAACACGATGGTTCGGACCTTTATCTGAGCACCGGTGCGCCGCCCTG
CGCCAAGTTTCACGGCAGCCTCAAGCCCATTGACCGAACACCATTGGCTCCTGGCATCATCAAAGAAATCGCCTATTCGA
TTATGGACGATGAACAGGTTTCCGAGTTTGAGCAAGAGCTGGAAATGAATCTGGCGTATAGCATTCGTAGCGTCGGCCGC
TTCCGTATCAACATTTTCCGCCAGCGCAACGAAATCTCCATCGTCGCTCGCAACATTGTCACAGACATTCCCAATGCCGA
TGACCTGGGACTCCCATCCATCCTCAAGGATGTGGTGATGGCAAAACGCGGCCTGGTACTGTTTGTCGGGGCTACCGGTT
CAGGAAAATCAACCTCACTGGCGGCACTGATTGACCACCGCAATTCCAACTCGGCCGGCCACATCATCACCATTGAGGAC
CCGGTCGAATACATTCACCGGCACAAGAAAAGCATTGTGAACCAACGGGAAGTTGGCGTTGATACCCGCAGTTTCCAGAA
AGCGTTGAAAAACACTCTGCGCCAGGCACCCGACGTCATTCTGATAGGCGAAATCCGTGACCGGGAAACCATGGAACACG
CGCTTGCCTTTGCCGAGACAGGGCACCTGTGCATCTCAACACTGCACGCGAACAACGCCAACCAGGCGCTGGACCGGATC
ATTAACTTTTTTCCGGAAGAGCGTCGCGCCCAACTCCTGATGGACCTCGCGATGAACCTGCGCGCGTTTATATCGCAACG
TTTAATCCCAACGGTCGATAAAAAACGGTGTGCTGCCATCGAAATCCTGCTCGGCACTCCCATAATCAGCGAGCTGATCC
TTCGCGGCGAATTCGACGGCATCAAGGAGATCATGGAGAAGTCGGCAAATCTTGGGATGCAGACCTTCGATCTTGCTCTC
TTCAACCTTTGGAAAGACGGGAAGATTTCGGAGGAGGAAGCGCTCAAGAACGCCGATTCCGCAAACAACCTCCGCCTGAA
AATCAAACTGCATGGTCAAGGCGGCGCAGGAAGCACCTCATCGCCGAAAAGCGGTGCACCCTCCGGCAGCGGTCTGTCCC
TTCAGATGGAAGACGATGAGGACGACTCGCCCGAATAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-249)

3D structure

• AlphaFold DB: A0A1D9GJ44

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	54.856	98.961	0.543
pilU	Acinetobacter baylyi ADP1	55.966	91.429	0.512
pilU	Vibrio cholerae strain A1552	49.734	97.662	0.486
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.582	87.013	0.379
pilT	Pseudomonas aeruginosa PAK	41.279	89.351	0.369