Detailed information NTG0200656

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: CGZ53_RS00365
• Genome accession: NZ_CP022435
• Coordinates: 62701..63705 (+)
• Length: 334 a.a.
• NCBI ID: WP_012657625.1
• Uniprot ID: B9DSU1
• Organism: Streptococcus uberis strain NZ01
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 57701..68705

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CGZ53_RS00345 (CGZ53_00345)	purE	58285..58773 (+)	489	WP_012657620.1	5-(carboxyamino)imidazole ribonucleotide mutase	-
CGZ53_RS00350 (CGZ53_00350)	purK	58760..59833 (+)	1074	WP_046389804.1	5-(carboxyamino)imidazole ribonucleotide synthase	-
CGZ53_RS00355 (CGZ53_00355)	purB	60147..61439 (+)	1293	WP_012657622.1	adenylosuccinate lyase	-
CGZ53_RS00360 (CGZ53_00360)	comR	61558..62472 (+)	915	WP_249630218.1	helix-turn-helix domain-containing protein	Regulator
CGZ53_RS09490	-	62571..62669 (+)	99	WP_198433762.1	quorum-sensing system DWW-type pheromone	-
CGZ53_RS00365 (CGZ53_00365)	ruvB	62701..63705 (+)	1005	WP_012657625.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
CGZ53_RS00370 (CGZ53_00370)	-	63956..64384 (+)	429	WP_012657626.1	low molecular weight protein-tyrosine-phosphatase	-
CGZ53_RS00375 (CGZ53_00375)	-	64389..64802 (+)	414	WP_012657627.1	membrane protein	-
CGZ53_RS00380 (CGZ53_00380)	-	64799..66577 (+)	1779	WP_100911829.1	acyltransferase family protein	-

Sequence

Protein

• Length: 334 a.a.
• Molecular weight: 37690.33 Da
• Isoelectric Point: 4.6255

>NTDB_id=200656 CGZ53_RS00365 WP_012657625.1 62701..63705(+) (ruvB) [Streptococcus uberis strain NZ01]
MTRILDNDLMGDEELIERTLRPQYLKEYIGQDKVKNQLSIFIEAAKLRDESLDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPAIEKAGDLVAILNDLEPGDVLFIDEIHRMPMAVEEVLYSAMEDFYIDIMIGAGDTSRSVHLELPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYQVDDLTEIVERTSDIFEMAIVHEAALELAKRSRGTPRIANRLLKRVRDYAQIMGDGVIT
KEMTDKALEMLDVDHEGLDYVDQKILRTMIEMYNGGPVGLGTLSVNIAEERDTVEDMYEPYLIQKGFIMRTRTGRVATEK
AYRHLKYPLDTKTE

Nucleotide

• Length: 1005 bp

>NTDB_id=200656 CGZ53_RS00365 WP_012657625.1 62701..63705(+) (ruvB) [Streptococcus uberis strain NZ01]
ATGACTAGAATATTAGATAACGATTTAATGGGTGATGAAGAGTTAATTGAACGGACGCTTCGCCCTCAATACCTGAAAGA
ATACATTGGACAAGATAAAGTTAAAAATCAACTGTCTATCTTTATTGAAGCAGCGAAATTAAGAGATGAATCACTTGATC
ATGTCTTACTCTTTGGGCCTCCGGGCCTAGGTAAGACAACCATGGCATTCGTTATTGCTAATGAGCTTGGTGTTAATTTA
AAACAAACTTCCGGTCCAGCTATTGAAAAGGCAGGAGACCTTGTTGCTATTTTAAATGATTTAGAACCCGGAGATGTTTT
ATTTATTGATGAGATTCATCGTATGCCAATGGCTGTTGAAGAAGTACTATATAGCGCCATGGAAGATTTTTACATTGATA
TTATGATTGGAGCAGGAGACACAAGTAGGAGTGTTCATCTAGAACTTCCTCCTTTTACATTAATTGGAGCAACGACTCGT
GCGGGGATGTTATCAAATCCTCTAAGAGCTCGCTTTGGAATCACTGGGCATATGGAATATTATCAGGTTGATGATCTGAC
GGAAATTGTGGAACGTACCTCTGATATCTTCGAAATGGCTATTGTCCATGAAGCTGCTTTGGAATTAGCAAAACGTAGTC
GAGGAACCCCCCGAATTGCTAACCGCTTATTAAAAAGAGTTCGAGATTATGCTCAGATTATGGGTGATGGGGTAATCACG
AAAGAAATGACCGACAAGGCTTTAGAAATGCTTGATGTTGATCATGAAGGTTTAGATTATGTCGATCAGAAAATTCTCAG
AACCATGATTGAAATGTATAACGGTGGTCCAGTGGGATTAGGAACCTTATCTGTCAATATTGCTGAAGAAAGAGATACTG
TTGAAGATATGTATGAGCCTTATCTTATTCAGAAAGGGTTTATTATGAGAACCAGAACGGGCCGAGTAGCAACAGAAAAA
GCCTACCGTCACCTGAAGTACCCATTAGATACAAAAACGGAATAG

Domains

Predicted by InterProScan.

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-326)

• RuvB AAA lid domain (AAA_lid_4) (181-254)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

3D structure

• AlphaFold DB: B9DSU1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae TIGR4	88.253	99.401	0.877
ruvB	Streptococcus pneumoniae R6	87.952	99.401	0.874
ruvB	Streptococcus pneumoniae D39	87.952	99.401	0.874
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.185	97.006	0.584
ruvB	Helicobacter pylori 26695	49.387	97.605	0.482
ruvB	Synechocystis sp. PCC 6803	49.515	92.515	0.458