Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   BHW77_RS27545 Genome accession   NZ_CP017251
Coordinates   5393542..5395062 (+) Length   506 a.a.
NCBI ID   WP_000058995.1    Uniprot ID   A0AAV3H3B5
Organism   Escherichia coli strain NADC 5570/86-24/6564 isolate wild     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 5388542..5400062
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BHW77_RS27525 (BHW77_27525) ilvE 5390096..5391025 (-) 930 WP_000208520.1 branched-chain-amino-acid transaminase -
  BHW77_RS27530 (BHW77_27530) ilvM 5391045..5391308 (-) 264 WP_000983255.1 acetolactate synthase 2 small subunit -
  BHW77_RS27535 (BHW77_27535) ilvG 5391305..5392951 (-) 1647 WP_001012623.1 acetolactate synthase 2 catalytic subunit -
  BHW77_RS31530 ilvX 5392954..5393004 (-) 51 WP_100635202.1 peptide IlvX -
  BHW77_RS27540 (BHW77_27540) ilvL 5393091..5393189 (-) 99 WP_001311244.1 ilv operon leader peptide -
  BHW77_RS27545 (BHW77_27545) comM 5393542..5395062 (+) 1521 WP_000058995.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  BHW77_RS27550 (BHW77_27550) maoP 5395087..5395425 (-) 339 WP_000841001.1 macrodomain Ori organization protein MaoP -
  BHW77_RS27555 (BHW77_27555) hdfR 5395544..5396383 (+) 840 WP_000379246.1 HTH-type transcriptional regulator HdfR -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55217.37 Da        Isoelectric Point: 7.6190

>NTDB_id=196960 BHW77_RS27545 WP_000058995.1 5393542..5395062(+) (comM) [Escherichia coli strain NADC 5570/86-24/6564 isolate wild]
MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKEARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGR
YDLPIAIALLAASEQLTANKLDEYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLVADHL
QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGPPGTGKTMLASRINGLLPDLSNE
EALESAAILSLVNAESVQKQWRQRPFRSPHHSASLTAMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIES
GQIHLSRTRAKITYPARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGILSKTVVPG
ESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRKFCKLESEDAQWLEETLIHLGLSIRAWQRLLKVARTIADIDQSDII
TRQHLQEAVSYRAIDRLLIHLQKLLT

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=196960 BHW77_RS27545 WP_000058995.1 5393542..5395062(+) (comM) [Escherichia coli strain NADC 5570/86-24/6564 isolate wild]
ATGTCACTGTCAATTGTTCATACCCGCGCAGCCCTGGGAGTAAATGCGCCCCCAATCACTGTTGAGGTACATATCAGTAA
AGGTTTGCCCGGCTTAACGATGGTGGGCTTACCAGAAACAACGGTAAAAGAAGCTCGCGATCGCGTGCGCAGCGCCATTA
TCAATAGCGGATATGAATATCCGGCGAAAAAAATCACCATTAACCTTGCTCCAGCCGATCTACCAAAAGAAGGGGGACGA
TATGATTTACCTATCGCCATTGCGTTGCTGGCGGCCTCAGAACAGCTTACAGCCAATAAGTTAGATGAATATGAATTAGT
CGGAGAACTGGCGCTTACAGGCGCTCTGCGTGGCGTTCCCGGTGCAATCTCCAGTGCAACTGAAGCTATTAAGTCGGGCA
GAAAAATTATCGTCGCGAAAGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGGTAGCCGATCATCTG
CAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACA
ACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAGGACTGGAAATTACCGCCGCTGGCGGGCACAACCTTT
TACTGATTGGGCCGCCGGGAACAGGTAAAACAATGCTCGCCAGCCGTATTAATGGTCTTTTGCCAGATTTAAGCAATGAA
GAGGCACTGGAGAGCGCTGCGATATTAAGTCTGGTAAATGCTGAATCAGTACAAAAACAATGGCGGCAGCGCCCGTTCCG
CTCACCTCATCACAGTGCGTCGTTAACGGCGATGGTGGGCGGTGGTGCAATTCCAGGGCCAGGTGAAATTTCGTTGGCGC
ATAACGGCGTGCTTTTTCTTGATGAGCTACCTGAATTTGAACGGCGTACACTGGATGCTTTGCGAGAGCCGATTGAATCC
GGGCAGATCCATCTTTCACGCACACGAGCAAAAATAACCTATCCAGCCCGTTTCCAGCTTGTTGCGGCGATGAATCCCAG
CCCTACCGGACATTATCAGGGAAACCATAACCGCTGCACGCCAGAACAGACATTACGTTATCTCAACCGGCTCTCGGGGC
CCTTTCTCGACCGCTTCGATCTCTCACTGGAGATCCCATTACCACCCCCCGGCATTTTGAGTAAAACGGTAGTGCCGGGA
GAAAGCAGCACCACCGTTAAACAACGTGTAATGGCTGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAGTTGAACGCCTG
GCTGGATAGTCCGGAAATACGCAAATTCTGCAAGCTTGAGAGCGAAGATGCGCAGTGGCTGGAAGAAACGCTGATCCATC
TGGGGTTATCGATTCGTGCCTGGCAGCGGTTATTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATC
ACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGTGCGATTGACCGTTTGCTCATCCATCTGCAAAAACTACTGACATA
A

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(189-386)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

58.513

100

0.591
  comM Glaesserella parasuis strain SC1401

58.383

100

0.585
  comM Vibrio cholerae strain A1552

58.964

99.209

0.585
  comM Vibrio campbellii strain DS40M4

57.968

99.209

0.575
  comM Legionella pneumophila str. Paris

48.193

98.419

0.474
  comM Legionella pneumophila strain ERS1305867

48.193

98.419

0.474
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

44.269

100

0.443

Multiple sequence alignment