Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BHS05_RS29500 Genome accession   NZ_CP017170
Coordinates   7193750..7194868 (-) Length   372 a.a.
NCBI ID   WP_140794794.1    Uniprot ID   -
Organism   Myxococcus xanthus strain GH5.1.9c20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7188750..7199868
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BHS05_RS29485 (BHS05_28750) - 7189412..7190848 (-) 1437 WP_140794792.1 sigma-54-dependent transcriptional regulator -
  BHS05_RS29490 (BHS05_28755) - 7190845..7192377 (-) 1533 WP_140796655.1 two-component system sensor histidine kinase NtrB -
  BHS05_RS29495 (BHS05_28760) - 7192439..7193692 (-) 1254 WP_140794793.1 type II secretion system F family protein -
  BHS05_RS29500 (BHS05_28765) pilT 7193750..7194868 (-) 1119 WP_140794794.1 type IV pilus twitching motility protein PilT Machinery gene
  BHS05_RS29505 (BHS05_28770) pilB 7194912..7196612 (-) 1701 WP_011555739.1 type IV-A pilus assembly ATPase PilB -
  BHS05_RS29510 (BHS05_28775) - 7196817..7197743 (-) 927 WP_140794795.1 bifunctional riboflavin kinase/FAD synthetase -
  BHS05_RS29515 (BHS05_28780) trmB 7197819..7198439 (+) 621 WP_140794796.1 tRNA (guanine(46)-N(7))-methyltransferase TrmB -
  BHS05_RS29520 (BHS05_28785) - 7198551..7199594 (+) 1044 WP_140794797.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 40653.03 Da        Isoelectric Point: 8.2933

>NTDB_id=196267 BHS05_RS29500 WP_140794794.1 7193750..7194868(-) (pilT) [Myxococcus xanthus strain GH5.1.9c20]
MANLHQLLKAMVEKGASDLHVTTGSPPQLRVDGELVPLKTAPLTPVETKQLCYSILTDAQKHKFEEENELDLSFGVKGLS
RFRANIFMQRGAVAAAFRTIPFKILTFQELGLPPVVAELVKKPRGLILVTGPTGSGKSTTLASMIDKINTERHEHIMTIE
DPIEYLHPHKNCLVNQREVGADTRNFKTALKYILRQDPDIVLVGELRDLETIEAALTIAETGHICYATLHTNSAVQTINR
VLDVFPPYQQPQVRAQMSFVLEGVMSQALVAKAGGPGRILALEVMVPNPAIRNLIREDKVHQIYSSMQVGQAKFGMQTFN
QALAALLLRRLITQDEAFGRSSDAEELRNILATGGGGALPGMQRPGGGAGGR

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=196267 BHS05_RS29500 WP_140794794.1 7193750..7194868(-) (pilT) [Myxococcus xanthus strain GH5.1.9c20]
GTGGCCAACCTGCACCAGCTCCTCAAGGCGATGGTCGAGAAGGGCGCTTCCGACCTCCACGTCACCACCGGCTCTCCGCC
GCAGCTTCGCGTCGACGGCGAGCTCGTTCCCTTGAAGACGGCGCCGCTGACCCCGGTGGAGACGAAGCAGCTCTGCTACT
CCATCCTCACGGACGCCCAGAAGCACAAGTTCGAGGAGGAGAACGAGCTGGACCTGTCCTTCGGCGTGAAGGGCCTGTCG
CGCTTCCGCGCCAACATCTTCATGCAGCGTGGCGCGGTCGCCGCGGCGTTCCGGACCATTCCCTTCAAGATTCTGACGTT
CCAGGAGCTGGGGCTGCCGCCGGTGGTGGCGGAGCTGGTGAAGAAGCCGCGCGGGCTCATCCTGGTGACGGGCCCCACGG
GTTCAGGCAAGTCCACCACGCTGGCCTCGATGATCGACAAGATCAACACCGAGCGTCATGAGCACATCATGACCATCGAG
GACCCCATCGAGTACCTGCACCCGCACAAGAACTGCCTCGTGAATCAGCGCGAGGTGGGAGCGGACACGCGGAACTTCAA
GACGGCCCTCAAGTACATCCTCCGCCAGGACCCGGACATCGTGCTCGTCGGTGAGCTTCGCGACCTGGAGACGATTGAGG
CGGCGCTCACCATCGCCGAGACGGGTCACATCTGCTACGCGACGCTACACACCAATAGCGCGGTGCAGACCATCAACCGC
GTGCTGGACGTGTTCCCGCCGTACCAGCAGCCGCAGGTCCGCGCGCAGATGTCCTTCGTGCTGGAAGGCGTGATGAGCCA
GGCGCTGGTCGCGAAGGCGGGCGGCCCTGGCCGCATCCTGGCGCTGGAGGTGATGGTGCCCAACCCCGCAATCCGGAACC
TCATCCGCGAGGACAAGGTCCACCAGATCTACTCGTCCATGCAGGTCGGCCAGGCGAAGTTCGGCATGCAGACCTTCAAC
CAGGCCCTGGCGGCGCTGCTGCTGCGGCGGCTCATCACCCAGGACGAGGCCTTCGGACGCTCTAGCGACGCGGAGGAGCT
GCGCAACATCCTGGCCACGGGCGGCGGCGGGGCCCTGCCCGGGATGCAGCGACCAGGCGGGGGAGCGGGTGGTCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.696

96.505

0.489
  pilT Legionella pneumophila strain ERS1305867

53.134

90.054

0.478
  pilT Legionella pneumophila strain Lp02

53.134

90.054

0.478
  pilT Pseudomonas aeruginosa PAK

52.537

90.054

0.473
  pilT Pseudomonas stutzeri DSM 10701

52.537

90.054

0.473
  pilT Acinetobacter baumannii D1279779

52.266

88.978

0.465
  pilT Acinetobacter baumannii strain A118

52.266

88.978

0.465
  pilT Acinetobacter nosocomialis M2

52.266

88.978

0.465
  pilT Acinetobacter baylyi ADP1

52.424

88.71

0.465
  pilT Vibrio cholerae strain A1552

48.204

89.785

0.433
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.204

89.785

0.433
  pilT Neisseria meningitidis 8013

44.575

91.667

0.409
  pilT Neisseria gonorrhoeae MS11

44.575

91.667

0.409
  pilU Vibrio cholerae strain A1552

43.059

94.892

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.741

94.355

0.384
  pilU Acinetobacter baylyi ADP1

39.193

93.28

0.366

Multiple sequence alignment