Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   CDC27_RS15925 Genome accession   NZ_CP021726
Coordinates   3130092..3131585 (-) Length   497 a.a.
NCBI ID   WP_072685947.1    Uniprot ID   -
Organism   Escherichia coli strain Combat11I9     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3125092..3136585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDC27_RS15895 (CDC27_15880) gspK 3125886..3126863 (-) 978 WP_072685937.1 type II secretion system minor pseudopilin GspK -
  CDC27_RS15900 (CDC27_15885) gspJ 3126860..3127465 (-) 606 WP_072685939.1 type II secretion system minor pseudopilin GspJ -
  CDC27_RS15905 (CDC27_15890) gspI 3127462..3127833 (-) 372 WP_072685941.1 type II secretion system minor pseudopilin GspI -
  CDC27_RS15910 (CDC27_15895) gspH 3127830..3128393 (-) 564 WP_072685943.1 type II secretion system minor pseudopilin GspH -
  CDC27_RS15915 (CDC27_15900) gspG 3128397..3128852 (-) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  CDC27_RS15920 (CDC27_15905) gspF 3128869..3130092 (-) 1224 WP_072685945.1 type II secretion system inner membrane protein GspF -
  CDC27_RS15925 (CDC27_15910) pilB/pilB1 3130092..3131585 (-) 1494 WP_072685947.1 type II secretion system ATPase GspE Machinery gene
  CDC27_RS15930 (CDC27_15915) gspD 3131585..3133645 (-) 2061 WP_072685950.1 type II secretion system secretin GspD -
  CDC27_RS15935 (CDC27_15920) gspC 3133675..3134634 (-) 960 WP_072685952.1 type II secretion system protein GspC -
  CDC27_RS15940 (CDC27_15925) gspS2 3134652..3135062 (-) 411 WP_001345954.1 type II secretion system pilot lipoprotein GspS-beta -
  CDC27_RS15945 (CDC27_15930) pilD 3135128..3135937 (-) 810 WP_000895883.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55192.35 Da        Isoelectric Point: 6.0390

>NTDB_id=195484 CDC27_RS15925 WP_072685947.1 3130092..3131585(-) (pilB/pilB1) [Escherichia coli strain Combat11I9]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQAAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYIRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=195484 CDC27_RS15925 WP_072685947.1 3130092..3131585(-) (pilB/pilB1) [Escherichia coli strain Combat11I9]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGCTATCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAAGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAAGCTCGCCAGTTGATGGAAGATCTCGGTTCTGCTGAGGATTTTTTTACCCTCGCCGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGCGACGACGACGCGCCAATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGCTGCGC
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATTAAGGTGATGGCGCGGCTGGATATTGCCGAAAAGCGCGTACC
GCAGGATGGGCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCTGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCGTCGCTTACCGGGCATCT
GGTGCTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTCCGCACGCTGTGCCCCGACTGCCGCCAGGCCGCGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCCCGTACCGTCACTCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATATCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.372

100

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.294

85.513

0.404
  pilB Vibrio parahaemolyticus RIMD 2210633

48.615

79.879

0.388
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Acinetobacter baumannii D1279779

46.366

80.282

0.372
  pilB Vibrio cholerae strain A1552

46.465

79.678

0.37
  pilB Acinetobacter baylyi ADP1

46.154

78.471

0.362
  pilF Thermus thermophilus HB27

45

80.483

0.362

Multiple sequence alignment