Detailed information NTG0194298

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: AM396_RS20150
• Genome accession: NZ_CP021532
• Coordinates: 3977496..3978488 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain AR_0149
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 3972496..3983488

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AM396_RS20130 (AM396_20090)	otnK	3973271..3974533 (+)	1263	WP_000590403.1	3-oxo-tetronate kinase	-
AM396_RS20135 (AM396_20095)	ygbL	3974530..3975168 (+)	639	WP_001278994.1	aldolase	-
AM396_RS20140 (AM396_20100)	ygbM	3975173..3975949 (+)	777	WP_001136934.1	HPr family phosphocarrier protein	-
AM396_RS20145 (AM396_20105)	ygbN	3976038..3977402 (+)	1365	WP_000104441.1	GntP family transporter	-
AM396_RS20150 (AM396_20110)	rpoS	3977496..3978488 (-)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
AM396_RS20155 (AM396_20115)	nlpD	3978551..3979645 (-)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
AM396_RS20160 (AM396_20120)	pcm	3979830..3980456 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
AM396_RS20165 (AM396_20125)	surE	3980450..3981211 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
AM396_RS20170 (AM396_20130)	truD	3981192..3982241 (-)	1050	WP_000568943.1	tRNA pseudouridine(13) synthase TruD	-
AM396_RS20175 (AM396_20135)	ispF	3982238..3982717 (-)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
AM396_RS20180 (AM396_20140)	ispD	3982717..3983427 (-)	711	WP_000246138.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=194298 AM396_RS20150 WP_000081550.1 3977496..3978488(-) (rpoS) [Escherichia coli strain AR_0149]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=194298 AM396_RS20150 WP_000081550.1 3977496..3978488(-) (rpoS) [Escherichia coli strain AR_0149]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715