Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BFG52_RS01010 Genome accession   NZ_CP016895
Coordinates   241347..243125 (-) Length   592 a.a.
NCBI ID   WP_081408587.1    Uniprot ID   -
Organism   Acinetobacter larvae strain BRTC-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 236347..248125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BFG52_RS00985 (BFG52_00985) rlmB 236676..237425 (+) 750 WP_067551383.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  BFG52_RS00990 (BFG52_00990) - 237484..238470 (+) 987 WP_067551386.1 DMT family transporter -
  BFG52_RS00995 (BFG52_00995) coaE 238501..239118 (-) 618 WP_067551389.1 dephospho-CoA kinase -
  BFG52_RS01000 (BFG52_01000) pilD 239162..240064 (-) 903 WP_067551392.1 A24 family peptidase Machinery gene
  BFG52_RS01005 (BFG52_01005) - 240081..241337 (-) 1257 WP_067551395.1 type II secretion system F family protein -
  BFG52_RS01010 (BFG52_01010) pilB 241347..243125 (-) 1779 WP_081408587.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BFG52_RS01015 (BFG52_01015) tpiA 243480..244271 (+) 792 WP_067551398.1 triose-phosphate isomerase -
  BFG52_RS01020 (BFG52_01020) secG 244285..244620 (+) 336 WP_067551401.1 preprotein translocase subunit SecG -
  BFG52_RS01040 (BFG52_01040) rimP 245242..245766 (+) 525 WP_067551404.1 ribosome maturation factor RimP -
  BFG52_RS01045 (BFG52_01045) nusA 245800..247284 (+) 1485 WP_067551407.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 592 a.a.        Molecular weight: 66199.80 Da        Isoelectric Point: 6.5285

>NTDB_id=193488 BFG52_RS01010 WP_081408587.1 241347..243125(-) (pilB) [Acinetobacter larvae strain BRTC-1]
MARFDRAEHPILPALNQQIVLSGLLRRFVEQQLLSQDAMLLAMQQAEAQQKHIIAYLVDELHLNAAQLAQQIGLEFNETV
LDLSHYDPDLLPKNLINPSLLHYYQVLPLGITQDRLRLAMSDPSRLDAIQAIGFHSNLNIEPVLVTHDQLCHCLDQYFPD
HHDHNFQEQDLAQEAHQGTTTEELIPASLVSHNEDTPIVVRYLNKLVADALHLGASDLHFEPYANSYRVRYRIDGLLHSM
SSPPPQLALRIAARLKVMAQLDIAEKRIPQDGRIRFHGLGQHVIDLRISTLPTLFGEKIVLRILDTRNSHFSFEQLGLNP
QQSQLFLDNLYKPQGMLLITGPTGSGKTASLYSGLRLLNQQHLNISTVEDPVEIQLEGINQVNVNPKAGLDFATALKAFL
RQDPDIMMLGEIRDIDTAELAIKAAQTGHLVLSTLHTNSAAETLTRLRHMGIAAFNIASAVNLVIAQRLLRRLCPHCKYA
LDVPLDSLYAMGFHASQLQHHPTLYQAAQCNKCHDGYKGRFAIYEMLPITPEIHQLILRDASATAIAQQAEQQGFNNLHQ
AALTQVLQGQTSLQEVNRVLSHALNPVTSSIV

Nucleotide


Download         Length: 1779 bp        

>NTDB_id=193488 BFG52_RS01010 WP_081408587.1 241347..243125(-) (pilB) [Acinetobacter larvae strain BRTC-1]
ATGGCTCGCTTTGATCGCGCAGAACACCCCATACTTCCAGCGCTCAATCAGCAGATTGTGCTGTCTGGCTTACTCAGACG
CTTCGTGGAACAGCAATTACTCAGTCAAGATGCCATGCTACTCGCCATGCAACAGGCTGAGGCACAGCAAAAGCACATCA
TTGCTTATCTGGTCGATGAACTTCACTTAAATGCCGCCCAACTCGCCCAGCAAATCGGTTTAGAATTCAATGAAACGGTA
CTGGATCTCTCTCATTATGACCCTGATCTATTGCCTAAAAATCTAATCAATCCGAGCTTATTACACTATTATCAGGTATT
ACCACTTGGCATTACACAGGATCGTTTGCGTTTAGCCATGAGTGACCCGAGTCGTCTCGATGCCATTCAAGCCATCGGCT
TTCATAGTAATCTCAACATAGAACCGGTATTGGTCACACATGATCAACTGTGCCATTGCTTAGATCAATATTTCCCAGAC
CATCATGATCATAATTTTCAGGAACAAGATCTGGCACAAGAAGCTCATCAAGGCACGACAACAGAAGAGCTTATTCCAGC
AAGTTTAGTGTCACATAATGAAGATACCCCCATCGTGGTGCGCTATCTCAACAAATTGGTCGCGGATGCACTGCATTTGG
GTGCATCGGATCTGCACTTTGAGCCCTATGCCAACAGTTATCGTGTACGTTACCGCATTGATGGTTTACTTCACAGCATG
AGCTCGCCGCCACCACAACTGGCTCTGCGGATTGCGGCGCGGCTCAAAGTCATGGCACAACTGGACATTGCCGAGAAACG
TATCCCACAAGATGGTCGCATTCGCTTTCATGGTTTAGGACAACATGTAATTGACCTACGTATCAGCACCTTACCGACTT
TATTTGGTGAAAAAATTGTGTTGAGGATTTTAGATACTCGCAATAGCCATTTCAGCTTTGAGCAACTGGGGCTAAACCCA
CAACAAAGCCAATTATTTTTAGACAACTTATATAAGCCACAAGGCATGTTACTGATTACAGGACCAACCGGCTCTGGTAA
AACCGCATCGCTCTATAGTGGTCTGCGCTTACTCAATCAGCAACATCTCAATATCAGTACCGTTGAAGACCCTGTAGAGA
TTCAGCTAGAAGGTATTAATCAAGTCAATGTCAATCCCAAAGCGGGTCTAGATTTTGCTACCGCACTGAAAGCATTTTTA
CGCCAAGATCCAGACATTATGATGCTGGGTGAAATTCGAGATATCGATACCGCAGAACTTGCCATTAAAGCAGCACAAAC
GGGGCATTTGGTGTTATCGACGTTACATACCAATAGTGCAGCAGAAACACTGACCCGGCTCAGACATATGGGCATCGCGG
CATTTAATATCGCCAGTGCAGTCAACTTAGTGATTGCCCAACGTTTATTACGGCGTTTATGTCCGCATTGTAAATATGCC
TTAGATGTTCCTTTAGACAGCCTATACGCGATGGGTTTCCATGCCTCGCAATTACAGCACCATCCAACCCTCTATCAAGC
AGCGCAATGCAACAAATGCCATGATGGCTATAAAGGGCGTTTTGCCATTTATGAAATGCTGCCGATCACGCCTGAAATTC
ACCAGCTGATTTTAAGGGATGCCAGTGCGACTGCCATTGCCCAACAGGCGGAGCAACAAGGTTTTAACAACTTACATCAA
GCAGCTTTGACTCAGGTACTGCAAGGGCAGACCTCTTTACAAGAAGTAAATCGCGTACTCAGCCATGCGCTCAACCCAGT
AACATCCAGCATTGTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(205-472)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(64-162)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.356

96.791

0.507
  pilB Acinetobacter baylyi ADP1

53.097

95.439

0.507
  pilB Legionella pneumophila strain ERS1305867

44.876

95.608

0.429
  pilB Vibrio cholerae strain A1552

46.617

89.865

0.419
  pilB Vibrio campbellii strain DS40M4

43.494

94.764

0.412
  pilB Vibrio parahaemolyticus RIMD 2210633

43.525

93.919

0.409
  pilF Neisseria gonorrhoeae MS11

42.152

95.777

0.404

Multiple sequence alignment