Detailed information NTG0193182

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BE844_RS13520
• Genome accession: NZ_CP016873
• Coordinates: 3020742..3021806 (+)
• Length: 354 a.a.
• NCBI ID: WP_011215094.1
• Uniprot ID: Q5WXY1
• Organism: Legionella pneumophila subsp. pneumophila strain FFI105
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3015742..3026806

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BE844_RS13510 (BE844_13510)	-	3016782..3019166 (-)	2385	WP_061690536.1	penicillin-binding protein 1A	-
BE844_RS13515 (BE844_13515)	ravI	3019318..3020325 (-)	1008	WP_011215093.1	Dot/Icm T4SS effector RavI	-
BE844_RS13520 (BE844_13520)	pilM	3020742..3021806 (+)	1065	WP_011215094.1	type IV pilus assembly protein PilM	Machinery gene
BE844_RS13525 (BE844_13525)	pilN	3021810..3022358 (+)	549	WP_010946663.1	PilN domain-containing protein	Machinery gene
BE844_RS13530 (BE844_13530)	pilO	3022372..3022971 (+)	600	WP_011215096.1	type 4a pilus biogenesis protein PilO	Machinery gene
BE844_RS13535 (BE844_13535)	pilP	3022968..3023552 (+)	585	WP_027266404.1	pilus assembly protein PilP	Machinery gene
BE844_RS13540 (BE844_13540)	pilQ	3023556..3025652 (+)	2097	WP_011215098.1	type IV pilus secretin PilQ	Machinery gene
BE844_RS13545 (BE844_13545)	aroK	3026098..3026625 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38760.78 Da
• Isoelectric Point: 5.0367

>NTDB_id=193182 BE844_RS13520 WP_011215094.1 3020742..3021806(+) (pilM) [Legionella pneumophila subsp. pneumophila strain FFI105]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=193182 BE844_RS13520 WP_011215094.1 3020742..3021806(+) (pilM) [Legionella pneumophila subsp. pneumophila strain FFI105]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAAGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTGGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTATCCCATAGATGAAATAAATCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
GGCAAGTGGGCAAGACAAGATTGTTGCAATAGTAGATATTGGAGCCAATTATACCCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCGATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGATAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCATGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5WXY1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	100	100	1
comM	Acinetobacter nosocomialis M2	42.373	100	0.424
pilM	Acinetobacter baumannii D1279779	42.09	100	0.421
comM	Acinetobacter baylyi ADP1	40.678	100	0.407