Detailed information NTG0193130

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BE843_RS05265
• Genome accession: NZ_CP016872
• Coordinates: 1165728..1166792 (-)
• Length: 354 a.a.
• NCBI ID: WP_011215094.1
• Uniprot ID: Q5WXY1
• Organism: Legionella pneumophila subsp. pneumophila strain FFI104
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1160728..1171792

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BE843_RS05240 (BE843_05240)	aroK	1160909..1161436 (-)	528	WP_010946667.1	shikimate kinase AroK	-
BE843_RS05245 (BE843_05245)	pilQ	1161882..1163978 (-)	2097	WP_011215098.1	type IV pilus secretin PilQ	Machinery gene
BE843_RS05250 (BE843_05250)	pilP	1163982..1164566 (-)	585	WP_027266404.1	pilus assembly protein PilP	Machinery gene
BE843_RS05255 (BE843_05255)	pilO	1164563..1165162 (-)	600	WP_011215096.1	type 4a pilus biogenesis protein PilO	Machinery gene
BE843_RS05260 (BE843_05260)	pilN	1165176..1165724 (-)	549	WP_010946663.1	PilN domain-containing protein	Machinery gene
BE843_RS05265 (BE843_05265)	pilM	1165728..1166792 (-)	1065	WP_011215094.1	type IV pilus assembly protein PilM	Machinery gene
BE843_RS05270 (BE843_05270)	ravI	1167209..1168216 (+)	1008	WP_011215093.1	Dot/Icm T4SS effector RavI	-
BE843_RS05275 (BE843_05275)	-	1168368..1170752 (+)	2385	WP_061690536.1	penicillin-binding protein 1A	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38760.78 Da
• Isoelectric Point: 5.0367

>NTDB_id=193130 BE843_RS05265 WP_011215094.1 1165728..1166792(-) (pilM) [Legionella pneumophila subsp. pneumophila strain FFI104]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=193130 BE843_RS05265 WP_011215094.1 1165728..1166792(-) (pilM) [Legionella pneumophila subsp. pneumophila strain FFI104]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAAGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTGGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTATCCCATAGATGAAATAAATCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
GGCAAGTGGGCAAGACAAGATTGTTGCAATAGTAGATATTGGAGCCAATTATACCCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCGATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGATAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCATGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5WXY1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	100	100	1
comM	Acinetobacter nosocomialis M2	42.373	100	0.424
pilM	Acinetobacter baumannii D1279779	42.09	100	0.421
comM	Acinetobacter baylyi ADP1	40.678	100	0.407