Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BA022_RS15785 Genome accession   NZ_CP016278
Coordinates   3370914..3371417 (+) Length   167 a.a.
NCBI ID   WP_088888077.1    Uniprot ID   -
Organism   Diaphorobacter nitroreducens strain SL-205     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3365914..3376417
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BA022_RS15760 (BA022_15425) sucC 3365944..3367104 (+) 1161 WP_011807128.1 ADP-forming succinate--CoA ligase subunit beta -
  BA022_RS15765 (BA022_15430) sucD 3367124..3368017 (+) 894 WP_011807127.1 succinate--CoA ligase subunit alpha -
  BA022_RS15770 (BA022_15435) - 3368174..3368857 (+) 684 WP_088888074.1 TerC family protein -
  BA022_RS15775 (BA022_15440) - 3369139..3369954 (+) 816 WP_088888075.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  BA022_RS15780 (BA022_15445) - 3370059..3370712 (+) 654 WP_088888076.1 FHA domain-containing protein -
  BA022_RS15785 (BA022_15450) pilA 3370914..3371417 (+) 504 WP_088888077.1 pilin Machinery gene
  BA022_RS15790 (BA022_15455) tfpZ 3371475..3372254 (+) 780 WP_148665186.1 TfpX/TfpZ family type IV pilin accessory protein -
  BA022_RS15795 (BA022_15460) - 3372300..3373778 (+) 1479 WP_187305977.1 PglL family O-oligosaccharyltransferase -
  BA022_RS15800 (BA022_15465) moaC 3373803..3374306 (-) 504 WP_088888080.1 cyclic pyranopterin monophosphate synthase MoaC -
  BA022_RS15805 (BA022_15470) - 3374418..3375982 (+) 1565 Protein_3144 M48 family metalloprotease -
  BA022_RS15810 (BA022_15475) - 3375950..3376306 (-) 357 WP_041836445.1 phage holin family protein -

Sequence

Protein


Download         Length: 167 a.a.        Molecular weight: 16827.28 Da        Isoelectric Point: 7.7829

>NTDB_id=186507 BA022_RS15785 WP_088888077.1 3370914..3371417(+) (pilA) [Diaphorobacter nitroreducens strain SL-205]
MKRMLQQGFTLIELMIVVAIIGILAAVALPAYQDYTVRARVSEGLVMASAAKVNVQDVLASGNPQDDASGYATGYTAPAA
TRNVASVGIDPTTGRITVSTTAAAGAGTVIITPNAPAGSALPSGVDPFTPPAQSVAWRCMVAGAQAGSFVGTQTGTLAAR
FAPSECK

Nucleotide


Download         Length: 504 bp        

>NTDB_id=186507 BA022_RS15785 WP_088888077.1 3370914..3371417(+) (pilA) [Diaphorobacter nitroreducens strain SL-205]
ATGAAGCGTATGCTCCAACAGGGTTTCACCCTGATCGAACTGATGATCGTGGTAGCCATCATCGGTATTCTGGCTGCTGT
GGCACTGCCTGCTTATCAAGACTACACCGTGCGCGCTCGCGTGTCGGAAGGTCTGGTGATGGCTTCTGCCGCCAAAGTGA
ACGTGCAGGACGTGCTGGCCTCCGGTAACCCGCAAGACGACGCCAGTGGGTATGCAACGGGCTATACGGCTCCTGCGGCA
ACGCGCAACGTGGCTAGCGTGGGTATTGATCCCACGACCGGCAGAATTACTGTCTCGACCACTGCTGCGGCAGGTGCTGG
CACCGTGATCATCACTCCCAACGCTCCGGCAGGCTCGGCTCTGCCTTCGGGTGTTGATCCCTTCACGCCTCCTGCACAAT
CGGTCGCTTGGCGTTGCATGGTTGCCGGCGCGCAGGCGGGCTCCTTCGTTGGTACGCAAACCGGCACCCTGGCGGCTCGT
TTCGCGCCCTCTGAGTGCAAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-167)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

49.419

100

0.509
  pilA2 Legionella pneumophila str. Paris

46.951

98.204

0.461
  pilA2 Legionella pneumophila strain ERS1305867

46.951

98.204

0.461
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.436

100

0.437
  comP Acinetobacter baylyi ADP1

37.5

100

0.377

Multiple sequence alignment