Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   A6B40_RS09610 Genome accession   NZ_CP016226
Coordinates   2038789..2040102 (-) Length   437 a.a.
NCBI ID   WP_176672242.1    Uniprot ID   -
Organism   Mannheimia varigena strain 177     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2033789..2045102
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6B40_RS09580 (A6B40_09485) ruvB 2034417..2035421 (-) 1005 WP_176672238.1 Holliday junction branch migration DNA helicase RuvB -
  A6B40_RS09585 (A6B40_09490) ruvA 2035432..2036037 (-) 606 WP_176672239.1 Holliday junction branch migration protein RuvA -
  A6B40_RS09590 (A6B40_09495) - 2036146..2036772 (-) 627 WP_176672240.1 LysE family transporter -
  A6B40_RS09595 (A6B40_09500) - 2036774..2037247 (-) 474 WP_176672241.1 phosphatidylglycerophosphatase A -
  A6B40_RS09600 (A6B40_09505) thiL 2037253..2038233 (-) 981 WP_025247433.1 thiamine-phosphate kinase -
  A6B40_RS09605 (A6B40_09510) nusB 2038296..2038709 (-) 414 WP_025216880.1 transcription antitermination factor NusB -
  A6B40_RS09610 (A6B40_09515) comE 2038789..2040102 (-) 1314 WP_176672242.1 type IV pilus secretin PilQ Machinery gene
  A6B40_RS09615 (A6B40_09520) - 2040111..2040524 (-) 414 WP_176672243.1 hypothetical protein -
  A6B40_RS09620 (A6B40_09525) - 2040526..2041059 (-) 534 WP_176672244.1 hypothetical protein -
  A6B40_RS09625 (A6B40_09530) - 2041061..2041582 (-) 522 WP_176672245.1 hypothetical protein -
  A6B40_RS09630 (A6B40_09535) - 2041558..2042259 (-) 702 WP_176672246.1 competence protein ComA -
  A6B40_RS09635 (A6B40_09540) - 2042385..2044937 (+) 2553 WP_176672247.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 437 a.a.        Molecular weight: 48257.22 Da        Isoelectric Point: 7.5104

>NTDB_id=186407 A6B40_RS09610 WP_176672242.1 2038789..2040102(-) (comE) [Mannheimia varigena strain 177]
MFRVFIYLLFSSSLALADTISLAVKNAPTSLLLTYLSEEIGKNIVLEDDIQTKSTLRLENKSIEEIFKTISKVNKLSFSQ
ESDIVYISKKEEKLADLNSAPIAHLQNNGQNPPLVTTPKLMTKTIKLNYAKASEVIESLTKGSGTFLSENGYIHFDERSN
SLIIKDSAKSLQNIERLVKKLDQPTEQIAIEARIVTISSEHLQELGVRWGMFSRGADHYKFGGQLEGNGLSNVANNLNVN
FPVANGASAVLQVASINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLG
LEVVPHLSTQNQILLDLVVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQYLAQKGEDKVPVLGSIPFIK
RLFSQTRDKISKRELVIFVTPYILQSNEKVSSTKKSK

Nucleotide


Download         Length: 1314 bp        

>NTDB_id=186407 A6B40_RS09610 WP_176672242.1 2038789..2040102(-) (comE) [Mannheimia varigena strain 177]
ATGTTCAGAGTTTTTATTTATTTATTATTTAGCTCTTCACTAGCGTTGGCTGATACTATTTCTCTTGCAGTAAAAAATGC
ACCAACTTCACTTCTGCTTACTTATTTATCCGAAGAAATAGGGAAAAATATAGTATTAGAGGATGATATTCAAACTAAAT
CCACCTTAAGATTAGAAAATAAGTCTATTGAGGAGATATTCAAAACGATCAGTAAAGTAAATAAATTATCTTTTAGCCAA
GAAAGTGATATTGTTTATATCAGTAAAAAAGAAGAAAAATTGGCTGATTTAAATTCAGCTCCAATAGCCCATTTGCAAAA
TAACGGACAAAATCCACCGCTTGTAACCACTCCAAAGTTGATGACTAAAACGATAAAGCTCAATTATGCGAAAGCCTCAG
AAGTGATTGAATCTTTAACTAAAGGTAGTGGTACGTTTTTATCTGAGAATGGCTATATCCATTTCGATGAGCGAAGTAAT
AGCTTAATTATTAAAGATAGTGCGAAATCACTGCAAAATATTGAGCGATTAGTCAAAAAACTCGACCAACCTACAGAACA
AATTGCGATTGAAGCTCGCATTGTTACTATCAGTAGCGAACATTTGCAAGAGCTGGGAGTGCGTTGGGGAATGTTTTCCA
GAGGAGCTGATCACTATAAATTTGGCGGGCAACTTGAAGGTAATGGATTAAGTAATGTAGCTAATAATCTCAATGTGAAT
TTCCCTGTTGCGAATGGAGCTTCCGCCGTTTTACAAGTGGCTTCCATTAATAGCCGTGTGCTAGATTTGGAACTTAGTGC
ATTAGAGCAGGAAAACAGTGTGGAAATTATTGCCAGTCCTCGTCTTTTAACCACGAATAAAAAACCTGCAAGCATCAAGC
AAGGCACAGAAATTCCTTATGTGATGTATAACACAAAATCAGAAGCAACCGATGTCGAATTTAAAGAAGCTGTGTTAGGA
TTGGAAGTTGTTCCTCATCTTTCTACTCAAAATCAAATTTTGCTGGATTTAGTCGTAACCCAAAACTCGCCGAACTCTCA
ATCCGGTAGTAGCGGTTTAATTACTATTGATAAGCAAGAACTCAACACACAAGTTTTTGCTAAACACGGGGAAACTATTG
TATTAGGTGGAATTTTTCAATATTTAGCCCAAAAAGGGGAAGATAAAGTGCCTGTTTTAGGTTCAATTCCCTTTATTAAG
CGTTTATTCAGCCAAACTAGAGATAAAATCAGCAAACGAGAACTCGTCATTTTCGTTACTCCCTACATTCTTCAATCAAA
CGAAAAAGTAAGCAGTACGAAAAAATCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(267-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(122-187)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

61.609

99.542

0.613
  comE Haemophilus influenzae Rd KW20

51.566

94.966

0.49
  comE Haemophilus influenzae 86-028NP

48.848

99.314

0.485
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.81

96.568

0.384
  pilQ Vibrio cholerae strain A1552

39.81

96.568

0.384
  pilQ Vibrio campbellii strain DS40M4

37.85

97.941

0.371

Multiple sequence alignment