Detailed information NTG0185518

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: A9P84_RS04970
• Genome accession: NZ_CP016030
• Coordinates: 1086804..1087868 (+)
• Length: 354 a.a.
• NCBI ID: WP_011213353.1
• Uniprot ID: A0A3A6VL78
• Organism: Legionella pneumophila strain OLDA
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1081804..1092868

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A9P84_RS04960 (A9P84_04955)	-	1082846..1085230 (-)	2385	WP_011213351.1	penicillin-binding protein 1A	-
A9P84_RS04965 (A9P84_04960)	-	1085380..1086387 (-)	1008	WP_011213352.1	hypothetical protein	-
A9P84_RS04970 (A9P84_04965)	pilM	1086804..1087868 (+)	1065	WP_011213353.1	type IV pilus assembly protein PilM	Machinery gene
A9P84_RS04975 (A9P84_04970)	pilN	1087872..1088420 (+)	549	WP_011213354.1	PilN domain-containing protein	Machinery gene
A9P84_RS04980 (A9P84_04975)	pilO	1088434..1089033 (+)	600	WP_011213355.1	type 4a pilus biogenesis protein PilO	Machinery gene
A9P84_RS04985 (A9P84_04980)	pilP	1089030..1089614 (+)	585	WP_011213356.1	pilus assembly protein PilP	Machinery gene
A9P84_RS04990 (A9P84_04985)	pilQ	1089618..1091714 (+)	2097	WP_011213357.1	type IV pilus secretin PilQ	Machinery gene
A9P84_RS04995 (A9P84_04990)	aroK	1092160..1092687 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38788.79 Da
• Isoelectric Point: 5.0367

>NTDB_id=185518 A9P84_RS04970 WP_011213353.1 1086804..1087868(+) (pilM) [Legionella pneumophila strain OLDA]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKRLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=185518 A9P84_RS04970 WP_011213353.1 1086804..1087868(+) (pilM) [Legionella pneumophila strain OLDA]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAGATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTCGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAATCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGACAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCACCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: A0A3A6VL78

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	99.718	100	0.997
comM	Acinetobacter nosocomialis M2	42.655	100	0.427
pilM	Acinetobacter baumannii D1279779	42.373	100	0.424
comM	Acinetobacter baylyi ADP1	40.96	100	0.41