Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A9P85_RS07610 Genome accession   NZ_CP016029
Coordinates   1702158..1703882 (+) Length   574 a.a.
NCBI ID   WP_011946523.1    Uniprot ID   -
Organism   Legionella pneumophila strain Pontiac     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1697158..1708882
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9P85_RS16705 - 1697512..1697919 (+) 408 WP_230304715.1 hypothetical protein -
  A9P85_RS07590 (A9P85_07590) - 1697937..1700033 (+) 2097 WP_230304716.1 putative bifunctional diguanylate cyclase/phosphodiesterase -
  A9P85_RS07595 (A9P85_07595) - 1700035..1700604 (-) 570 WP_011213801.1 hypoxanthine-guanine phosphoribosyltransferase -
  A9P85_RS07600 (A9P85_07600) - 1700647..1700973 (-) 327 WP_011213802.1 DUF1820 family protein -
  A9P85_RS07605 (A9P85_07605) - 1701156..1701899 (-) 744 WP_011213803.1 methyltransferase domain-containing protein -
  A9P85_RS07610 (A9P85_07610) pilB 1702158..1703882 (+) 1725 WP_011946523.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A9P85_RS07615 (A9P85_07615) pilC 1703888..1705108 (+) 1221 WP_027226540.1 type II secretion system F family protein Machinery gene
  A9P85_RS07620 (A9P85_07620) pilD 1705154..1706017 (+) 864 WP_011946525.1 prepilin peptidase Machinery gene
  A9P85_RS07625 (A9P85_07625) - 1706031..1707236 (-) 1206 WP_061363796.1 cyclic nucleotide-binding domain-containing protein -
  A9P85_RS07630 (A9P85_07630) - 1707344..1707937 (-) 594 WP_011213808.1 hypothetical protein -
  A9P85_RS07635 (A9P85_07635) - 1707953..1708408 (-) 456 WP_042233877.1 hypothetical protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62722.31 Da        Isoelectric Point: 5.5861

>NTDB_id=185484 A9P85_RS07610 WP_011946523.1 1702158..1703882(+) (pilB) [Legionella pneumophila strain Pontiac]
MALATEEYRLQGIGQLLVLEKLLDKTKAIELHKLAAAEKISLLQYIVKNKILSAEQIALTAAQNFGVPMLDINCIDVGTI
PVNLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSALIDNLLTAKESQGLSEYVE
DSGDLEGLEISADDEDQDIDTATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLS
SRITARIKVMSNLDISERRIPQDGGFKMKISKSRAIDFRVSTCPTSAGEKVVMRVLDSGAAKLGIEALGFNPVQRTNFLK
AIQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIM
VGEIRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTNQG
LIELGFKEADLVNLKLYKAVGCEQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKE
GITTIEEVNRVTVD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=185484 A9P85_RS07610 WP_011946523.1 1702158..1703882(+) (pilB) [Legionella pneumophila strain Pontiac]
ATGGCTCTAGCTACAGAAGAATATAGATTACAGGGAATCGGCCAGCTTCTCGTGCTGGAAAAGCTGCTAGACAAAACAAA
AGCAATTGAACTACACAAGTTGGCAGCAGCAGAAAAGATATCTTTGCTGCAATACATTGTAAAAAATAAAATATTATCTG
CTGAACAAATTGCACTGACGGCTGCTCAAAATTTTGGCGTACCCATGTTGGATATTAATTGCATTGATGTGGGCACCATT
CCTGTCAATCTGGTTAATGAGAAATTAATTAAACGTCATGCCATGGTTCCTCTTTTTAGTCGCGGCACCAATTTATATCT
CGCAACAGATGATCCTAGTAAACAGGCTTCATTAAAGGAAATTCAATTTCATACCGGATTAAATACTCATGCGATAGTTG
TAGAAACAGATAAACTTAGTGCTCTGATTGATAACCTGTTAACAGCAAAGGAAAGTCAGGGCTTATCAGAGTATGTTGAA
GACTCTGGAGATCTAGAAGGTTTGGAAATAAGTGCTGATGATGAAGATCAAGATATTGATACTGCAACATCAGTAACTGA
TGATGCACCTATCGTAAAATTCGTCAATAAAATTTTATTGGATGCGATAAGGCAAGGAGCTTCTGATATACACTTTGAGC
CTTATGAACGAGAATATCGAATCAGGTACAGGCAAGATGGTATTTTACATGAGGTCGCTACCCCTCCCGCAAGCTTGTCA
TCTCGTATTACAGCACGTATCAAGGTAATGTCTAATTTGGATATATCAGAACGGCGTATCCCCCAAGATGGTGGCTTCAA
AATGAAAATTTCCAAGTCACGAGCAATTGATTTCAGAGTGAGCACATGTCCAACCTCGGCTGGGGAAAAAGTGGTTATGC
GGGTTCTTGATTCTGGTGCTGCTAAATTAGGTATTGAAGCATTAGGATTTAATCCTGTTCAGCGAACAAATTTCCTGAAA
GCAATCCAGCGCCCACAAGGGATGATTTTAGTCACTGGCCCTACTGGTAGTGGTAAAACAGTCACTTTATATACGGCTTT
AAATATATTAAATACAATTGAGGTTAATATTTCAACAGCTGAAGATCCAGTTGAAATCAAAGTGCCTGGTATCAATCAGG
TTAATATTAATCCTAAAGCTGGCCTAACCTTTTCCGGTGCATTGCGTTCGTTTCTAAGGCAAGATCCTGATATTATCATG
GTTGGTGAGATACGAGACCTTGAAACAGCAGAGATTGCTATAAAAGCTGCTCAGACAGGGCATTTGGTTCTTTCTACTCT
GCACACAAACAGTGCCGCTGAAACTTTAAATCGTTTAGTAAATATGGGAATACCTACTTTTAACATCGCGAGTTCGGTTA
CCTTAATTATTGCGCAAAGGCTGGCAAGAAAATTATGCAATCAATGTAAGGCAGTGCGTGATGACTTTACCAATCAAGGC
TTGATTGAACTAGGTTTCAAGGAAGCTGATTTGGTTAATCTTAAATTATACAAAGCAGTTGGTTGTGAGCAATGTACAAG
TGGTTATCGCGGGCGAGTTGGGTTGTTTGAGGTTTTACCGATGACAAAAGAGCTTGGCCAATTGATTATGTCAGGTGGTA
ATTCGTTGGATATATTAAAGTTAGCTCAATCAGAAGGTATGCTGACTATCTTTCAATCGGGTATAGAAAAAGTTAAGGAA
GGAATCACGACCATAGAGGAGGTCAATCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-151)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

100

100

1
  pilB Acinetobacter baylyi ADP1

49.828

100

0.505
  pilB Acinetobacter baumannii D1279779

50.794

98.78

0.502
  pilB Vibrio cholerae strain A1552

50.741

94.077

0.477
  pilF Neisseria gonorrhoeae MS11

47.173

98.606

0.465
  pilB Vibrio campbellii strain DS40M4

47.756

97.038

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

48.98

93.902

0.46
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.509

92.16

0.364
  pilF Thermus thermophilus HB27

38.848

93.728

0.364

Multiple sequence alignment