Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   B5762_RS18980 Genome accession   NZ_CP020368
Coordinates   3670539..3671207 (-) Length   222 a.a.
NCBI ID   WP_001321683.1    Uniprot ID   -
Organism   Escherichia coli strain BLR(DE3)     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3665539..3676207
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5762_RS18950 (B5762_03808) rfaQ 3665717..3666775 (-) 1059 WP_000360401.1 lipopolysaccharide core heptosyltransferase RfaQ -
  B5762_RS18955 (B5762_03809) waaA 3667188..3668465 (+) 1278 WP_000891574.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  B5762_RS18960 (B5762_03810) coaD 3668473..3668952 (+) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  B5762_RS18965 (B5762_03811) mutM 3668991..3669800 (-) 810 WP_001114543.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  B5762_RS18970 (B5762_03812) rpmG 3669898..3670065 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  B5762_RS18975 (B5762_03813) rpmB 3670086..3670322 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  B5762_RS18980 (B5762_03814) radC 3670539..3671207 (-) 669 WP_001321683.1 DNA repair protein RadC Machinery gene
  B5762_RS18985 (B5762_03815) coaBC 3671379..3672599 (+) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  B5762_RS18990 (B5762_03816) dut 3672577..3673035 (+) 459 WP_000976070.1 dUTP diphosphatase -
  B5762_RS18995 (B5762_03817) slmA 3673142..3673738 (+) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  B5762_RS19000 (B5762_03818) pyrE 3673775..3674416 (-) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  B5762_RS19005 (B5762_03819) rph 3674482..3675198 (-) 717 WP_001247089.1 ribonuclease PH -
  B5762_RS19015 (B5762_03821) yicC 3675325..3676188 (+) 864 WP_000621336.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25257.41 Da        Isoelectric Point: 8.9890

>NTDB_id=185484 B5762_RS18980 WP_001321683.1 3670539..3671207(-) (radC) [Escherichia coli strain BLR(DE3)]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGKYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=185484 B5762_RS18980 WP_001321683.1 3670539..3671207(-) (radC) [Escherichia coli strain BLR(DE3)]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGTTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAAAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment