Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   A9E93_RS04595 Genome accession   NZ_CP015953
Coordinates   1013548..1015644 (+) Length   698 a.a.
NCBI ID   WP_011946023.1    Uniprot ID   A0A378K6J3
Organism   Legionella pneumophila strain E6_N     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1008548..1020644
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9E93_RS04570 (A9E93_04570) ravI 1009310..1010317 (-) 1008 WP_011946020.1 Dot/Icm T4SS effector RavI -
  A9E93_RS04575 (A9E93_04575) pilM 1010734..1011798 (+) 1065 WP_011215094.1 type IV pilus assembly protein PilM Machinery gene
  A9E93_RS04580 (A9E93_04580) pilN 1011802..1012350 (+) 549 WP_011213354.1 PilN domain-containing protein Machinery gene
  A9E93_RS04585 (A9E93_04585) pilO 1012364..1012963 (+) 600 WP_013101267.1 type 4a pilus biogenesis protein PilO Machinery gene
  A9E93_RS04590 (A9E93_04590) pilP 1012960..1013544 (+) 585 WP_013101268.1 pilus assembly protein PilP Machinery gene
  A9E93_RS04595 (A9E93_04595) pilQ 1013548..1015644 (+) 2097 WP_011946023.1 type IV pilus secretin PilQ Machinery gene
  A9E93_RS04600 (A9E93_04600) aroK 1016097..1016618 (+) 522 WP_027219629.1 shikimate kinase AroK -
  A9E93_RS04605 (A9E93_04605) aroB 1016605..1017714 (+) 1110 WP_013101269.1 3-dehydroquinate synthase -
  A9E93_RS04610 (A9E93_04610) - 1017711..1019162 (+) 1452 WP_027219627.1 SPOR domain-containing protein -
  A9E93_RS04615 (A9E93_04615) - 1019271..1019702 (+) 432 WP_010946670.1 universal stress protein -

Sequence

Protein


Download         Length: 698 a.a.        Molecular weight: 77497.79 Da        Isoelectric Point: 9.5395

>NTDB_id=184589 A9E93_RS04595 WP_011946023.1 1013548..1015644(+) (pilQ) [Legionella pneumophila strain E6_N]
MRKIIAVLILISASLSMAYPKDNSLISVKVLPLPENRVRIDFQFSKPLKQLPASFITQKPPRLVLDFISTDMQIPSDQKT
KTIQLGSLHSYNIVAVGDRVRAILELDRSVSYSGSTAGSVYSLVLNGKSNDLFENSKEVFITNQVVNAKHEIKRIDFRGI
EKQGGRVLIDVSDTGIPIDVTQVGKEIVVNFLNTRIPLNLMKRYDVSDFHSPVQIITMQQEGKKVRMTILSKGDYGHFVY
QVNKQFMVDVFPLTAEEIRQAKLKKQVFTGKRISLNFQNISIRAVLQLLADFTGINMVVSDKVQGDITLRLNDIPWDQAL
DIILTTQGLDKRRKGNVMLIDTKASMDKMEEEQLKSQQTIEKLEPIRSDLIQINYAKAADIAVLIKDKQNSLLSDRGKIS
VDARTNTIWIQDTGTKIEEVRELIKQLDIPVKQVLIEARIVEVTKDFSQDIGIRWGVSRPTHLSGTLEGANELARGTAPA
NVVPLERRLNLDLAAAPLTGATPASVGIALAKLNDNILLDLELSALESEGRAELISSPRLITTNQQAAVIESGEEIPYQE
ATSSGATAVAFKKAVLSLKVTPQITPDGKILMDLQINQDTPSPQTFLGVPAIITKEIQTNVLVNNGQTIVLGGIYKQDKN
KVINRIPFFGELPVVGILFSNRQITLKNEELLIFITPRIITNALSITTIEGREKDVYK

Nucleotide


Download         Length: 2097 bp        

>NTDB_id=184589 A9E93_RS04595 WP_011946023.1 1013548..1015644(+) (pilQ) [Legionella pneumophila strain E6_N]
GTGAGAAAAATTATCGCGGTTTTGATATTAATTAGTGCCAGTTTGAGTATGGCATATCCTAAAGATAATTCACTCATTTC
AGTAAAAGTATTACCATTGCCTGAAAACAGAGTGCGCATTGATTTTCAATTTTCCAAACCCTTAAAGCAGTTGCCCGCAA
GCTTTATTACTCAAAAGCCCCCACGTTTGGTGTTAGATTTTATCAGCACTGATATGCAAATTCCTTCAGATCAGAAAACT
AAAACCATTCAATTAGGCTCATTACATAGCTATAACATTGTTGCTGTTGGGGATAGAGTAAGAGCCATATTGGAGCTGGA
TCGTTCAGTGTCCTATTCTGGCAGTACGGCGGGTTCCGTTTATAGCCTTGTCCTGAATGGTAAAAGCAATGATTTATTTG
AAAATAGCAAGGAAGTATTCATAACGAACCAGGTTGTGAACGCCAAGCATGAAATTAAAAGAATTGATTTTCGTGGTATA
GAAAAGCAGGGCGGTAGAGTTTTAATTGATGTTTCTGATACGGGTATTCCAATTGATGTAACTCAAGTTGGGAAAGAAAT
AGTCGTTAATTTCCTGAATACCAGGATCCCTTTGAATTTAATGAAACGGTATGATGTTTCTGATTTTCACAGCCCTGTGC
AGATTATTACTATGCAACAGGAAGGAAAAAAGGTTCGTATGACCATTCTCAGCAAAGGGGATTATGGGCATTTTGTTTAT
CAGGTCAATAAACAATTTATGGTCGATGTCTTTCCTTTAACTGCGGAAGAAATTCGTCAGGCGAAATTGAAGAAACAGGT
ATTTACCGGTAAACGGATTTCTTTGAATTTCCAGAATATTAGTATTCGAGCTGTGCTTCAACTGCTGGCAGATTTTACCG
GAATCAATATGGTTGTCAGCGACAAAGTGCAAGGAGATATTACGCTAAGATTAAATGATATTCCCTGGGATCAGGCGCTG
GATATCATTTTAACCACACAAGGGTTAGATAAGCGCAGAAAGGGCAACGTCATGTTGATAGATACCAAGGCCTCCATGGA
TAAAATGGAGGAAGAACAACTCAAAAGTCAACAGACAATTGAAAAACTTGAGCCTATTCGTTCTGATTTAATACAGATTA
ATTATGCAAAGGCAGCAGATATTGCTGTTTTGATCAAGGATAAGCAAAATTCCCTATTATCAGACAGAGGAAAAATAAGT
GTTGATGCCAGAACGAATACAATCTGGATTCAGGATACTGGCACCAAAATTGAGGAAGTAAGGGAACTGATAAAGCAATT
GGATATCCCTGTTAAGCAAGTGCTTATTGAGGCAAGAATTGTTGAAGTAACTAAAGATTTTAGTCAAGATATTGGGATAC
GCTGGGGGGTATCAAGACCAACTCACTTAAGTGGTACATTGGAAGGTGCTAACGAGTTAGCGAGAGGAACAGCACCAGCT
AATGTTGTCCCCTTGGAAAGACGATTAAATCTTGATTTGGCAGCTGCTCCTTTAACTGGAGCTACACCGGCCTCGGTAGG
TATTGCTCTAGCCAAGCTTAATGATAACATTCTGCTTGATCTGGAATTGTCTGCTTTGGAAAGTGAAGGGCGTGCTGAAT
TAATATCCAGTCCCCGCCTGATTACTACGAATCAACAGGCAGCAGTCATCGAGTCTGGTGAGGAGATTCCCTATCAGGAG
GCAACATCGAGTGGAGCGACGGCTGTTGCATTTAAAAAAGCAGTGTTGAGTTTGAAAGTTACCCCGCAAATTACGCCTGA
CGGTAAAATTTTGATGGATTTACAGATAAATCAGGATACTCCTTCTCCACAAACATTCCTTGGTGTTCCAGCAATAATAA
CAAAGGAGATTCAGACGAATGTTCTGGTTAATAATGGACAAACCATAGTACTTGGGGGTATTTATAAGCAAGATAAGAAC
AAGGTAATCAATAGAATTCCTTTCTTTGGTGAGTTGCCTGTTGTCGGTATTTTATTTAGTAATAGACAAATCACATTGAA
AAATGAAGAATTACTTATTTTTATTACACCAAGAATAATAACGAATGCCCTATCGATAACAACGATAGAAGGACGTGAAA
AAGATGTTTATAAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(525-680)

AMIN domain (AMIN)

(39-113)

Bacterial type II/III secretion system short domain (Secretin_N)

(370-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378K6J3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Legionella pneumophila strain Lp02

99.427

100

0.994
  pilQ Legionella pneumophila strain ERS1305867

99.284

100

0.993
  comQ Acinetobacter baylyi ADP1

38.428

98.424

0.378
  pilQ Pseudomonas aeruginosa PAK

38.102

98.138

0.374

Multiple sequence alignment