Detailed information NTG0182934

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: LLJM1_RS06060
• Genome accession: NZ_CP015899
• Coordinates: 1102346..1103131 (-)
• Length: 261 a.a.
• NCBI ID: WP_021214114.1
• Uniprot ID: -
• Organism: Lactococcus cremoris strain JM1
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1097346..1108131

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLJM1_RS06025 (LLJM1_1150)	-	1097946..1098440 (+)	495	WP_011676092.1	NUDIX hydrolase	-
LLJM1_RS06030 (LLJM1_1151)	ybeY	1098531..1099019 (+)	489	WP_032951096.1	rRNA maturation RNase YbeY	-
LLJM1_RS06035 (LLJM1_1152)	-	1099003..1099458 (+)	456	WP_011676094.1	diacylglycerol kinase family protein	-
LLJM1_RS06040 (LLJM1_1153)	-	1099475..1100053 (+)	579	WP_011676095.1	nucleotidyltransferase family protein	-
LLJM1_RS06045 (LLJM1_1154)	comFC	1100111..1100761 (-)	651	WP_032951095.1	ComF family protein	Machinery gene
LLJM1_RS06050 (LLJM1_1155)	comFA	1100761..1101219 (-)	459	WP_081196108.1	helicase-related protein	Machinery gene
LLJM1_RS06055 (LLJM1_1156)	-	1101313..1102248 (+)	936	WP_031559015.1	IS30 family transposase	-
LLJM1_RS06060 (LLJM1_1157)	comFA	1102346..1103131 (-)	786	WP_021214114.1	DEAD/DEAH box helicase family protein	Machinery gene
LLJM1_RS06065 (LLJM1_1158)	-	1103188..1103823 (+)	636	WP_011676097.1	YigZ family protein	-
LLJM1_RS06070 (LLJM1_1159)	-	1103865..1104323 (+)	459	WP_063280772.1	VanZ family protein	-
LLJM1_RS06075 (LLJM1_1160)	-	1104372..1105319 (+)	948	WP_003130410.1	IS30 family transposase	-
LLJM1_RS06080 (LLJM1_1161)	-	1105511..1106530 (+)	1020	WP_032951092.1	DUF475 domain-containing protein	-
LLJM1_RS06085 (LLJM1_1162)	-	1106699..1107406 (+)	708	WP_237024150.1	5'-nucleotidase	-

Sequence

Protein

• Length: 261 a.a.
• Molecular weight: 29919.55 Da
• Isoelectric Point: 7.8588

>NTDB_id=182934 LLJM1_RS06060 WP_021214114.1 1102346..1103131(-) (comFA) [Lactococcus cremoris strain JM1]
MNLILENLLGRLLLEKDISAFYNFTDQVNNENKLIKICAMTSVNANKVRCNRCGTIHIKTNVKLPIGAFFCPTCLELGRV
RSDEYFYHLPQQDFSEKTYLRWTGKLTENQEKISNALCQQITNNQKRLVQAVTGAGKTEMIYQLIEQILSHGGSVGLASP
RIDVCIELHQRLSRDFTCQIPLLYHEGDSYFRSPLVVMTSHQLLRFKEAFDLLIIDEVDAFPFRDNDMLYFALENAKKIN
GNLLYLTATSTDKLDKQIKKA

Nucleotide

• Length: 786 bp

>NTDB_id=182934 LLJM1_RS06060 WP_021214114.1 1102346..1103131(-) (comFA) [Lactococcus cremoris strain JM1]
ATGAACTTAATTTTAGAAAATCTCCTTGGGCGTCTTTTACTAGAAAAAGATATTTCAGCTTTTTATAATTTTACTGACCA
AGTTAACAATGAAAACAAATTAATTAAAATTTGTGCAATGACATCTGTCAATGCTAATAAAGTAAGATGTAATCGATGTG
GCACGATTCATATAAAAACCAATGTTAAACTTCCGATTGGTGCTTTTTTCTGTCCAACATGTCTTGAACTTGGTCGTGTC
CGCTCAGATGAATATTTCTATCATCTCCCTCAACAAGACTTTTCAGAAAAAACTTATTTACGTTGGACAGGAAAATTAAC
AGAAAATCAAGAAAAAATTTCTAACGCTCTCTGTCAGCAAATTACTAATAATCAAAAACGACTTGTTCAAGCCGTTACTG
GTGCTGGTAAAACAGAAATGATATATCAGCTTATTGAACAAATTCTTAGCCATGGGGGTTCGGTCGGTTTAGCCAGCCCA
AGAATTGATGTATGCATTGAGCTTCATCAACGTTTATCACGTGATTTCACTTGTCAAATTCCCCTTTTGTATCATGAGGG
AGATTCTTATTTTCGTTCGCCTTTGGTCGTTATGACCAGTCACCAACTTTTACGTTTCAAAGAGGCCTTTGATTTGCTTA
TCATCGATGAAGTCGATGCCTTTCCTTTTAGAGATAATGACATGCTTTATTTCGCTCTTGAAAATGCTAAAAAAATTAAT
GGAAACTTGCTCTACTTAACCGCTACATCTACTGACAAATTAGACAAACAGATAAAAAAAGCATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (105-249)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	97.308	99.617	0.969
comFA/cflA	Streptococcus mitis SK321	49.802	96.935	0.483
comFA/cflA	Streptococcus pneumoniae D39	49.012	96.935	0.475
comFA/cflA	Streptococcus pneumoniae Rx1	49.012	96.935	0.475
comFA/cflA	Streptococcus pneumoniae R6	49.012	96.935	0.475
comFA/cflA	Streptococcus pneumoniae TIGR4	49.012	96.935	0.475
comFA/cflA	Streptococcus mitis NCTC 12261	48.617	96.935	0.471
comFA	Bacillus subtilis subsp. subtilis str. 168	37.453	100	0.383