Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   B2H83_RS00535 Genome accession   NZ_CP019953
Coordinates   119956..120396 (-) Length   146 a.a.
NCBI ID   WP_000360902.1    Uniprot ID   A7ZHJ7
Organism   Escherichia coli M8     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 114956..125396
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B2H83_RS00515 (B2H83_00535) coaE 115446..116066 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  B2H83_RS26660 - 116091..116135 (+) 45 WP_120795372.1 protein YacM -
  B2H83_RS00520 (B2H83_00540) guaC 116291..117334 (+) 1044 WP_001217338.1 GMP reductase -
  B2H83_RS00525 (B2H83_00545) hofC 117369..118571 (-) 1203 WP_000157234.1 protein transport protein HofC -
  B2H83_RS00530 (B2H83_00550) pilB 118561..119946 (-) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  B2H83_RS00535 (B2H83_00555) pilA 119956..120396 (-) 441 WP_000360902.1 prepilin peptidase-dependent pilin Machinery gene
  B2H83_RS00540 (B2H83_00565) nadC 120599..121492 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  B2H83_RS00545 (B2H83_00570) ampD 121580..122131 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  B2H83_RS00550 (B2H83_00575) ampE 122128..122982 (+) 855 WP_129541682.1 beta-lactamase regulator AmpE -
  B2H83_RS00555 (B2H83_00580) aroP 123025..124398 (-) 1374 WP_000969914.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15630.76 Da        Isoelectric Point: 4.5559

>NTDB_id=182473 B2H83_RS00535 WP_000360902.1 119956..120396(-) (pilA) [Escherichia coli M8]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=182473 B2H83_RS00535 WP_000360902.1 119956..120396(-) (pilA) [Escherichia coli M8]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZHJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377

Multiple sequence alignment