Detailed information NTG0178503

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: A5478_RS04730
• Genome accession: NZ_CP015342
• Coordinates: 1038510..1039574 (+)
• Length: 354 a.a.
• NCBI ID: WP_014843599.1
• Uniprot ID: A0A4T1VG56
• Organism: Legionella pneumophila strain D-7632
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1033510..1044574

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A5478_RS04720 (A5478_04720)	-	1034550..1036934 (-)	2385	WP_014843597.1	penicillin-binding protein 1A	-
A5478_RS04725 (A5478_04725)	-	1037086..1038093 (-)	1008	WP_014843598.1	hypothetical protein	-
A5478_RS04730 (A5478_04730)	pilM	1038510..1039574 (+)	1065	WP_014843599.1	type IV pilus assembly protein PilM	Machinery gene
A5478_RS04735 (A5478_04735)	pilN	1039578..1040126 (+)	549	WP_011213354.1	PilN domain-containing protein	Machinery gene
A5478_RS04740 (A5478_04740)	pilO	1040140..1040736 (+)	597	WP_027224065.1	type 4a pilus biogenesis protein PilO	Machinery gene
A5478_RS04745 (A5478_04745)	pilP	1040733..1041317 (+)	585	WP_014843601.1	pilus assembly protein PilP	Machinery gene
A5478_RS04750 (A5478_04750)	pilQ	1041321..1043417 (+)	2097	WP_014843602.1	type IV pilus secretin PilQ	Machinery gene
A5478_RS04755 (A5478_04755)	aroK	1043862..1044389 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38776.78 Da
• Isoelectric Point: 5.0367

>NTDB_id=178503 A5478_RS04730 WP_014843599.1 1038510..1039574(+) (pilM) [Legionella pneumophila strain D-7632]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLASLIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=178503 A5478_RS04730 WP_014843599.1 1038510..1039574(+) (pilM) [Legionella pneumophila strain D-7632]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAATCTTATGCAGTAGAGCGTTCGGTACAACAAATTGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAGCCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGCCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGACAACCAGGATTGGCATCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: A0A4T1VG56

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	99.718	100	0.997
comM	Acinetobacter nosocomialis M2	42.373	100	0.424
pilM	Acinetobacter baumannii D1279779	42.09	100	0.421
comM	Acinetobacter baylyi ADP1	40.678	100	0.407