Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   A5478_RS04750 Genome accession   NZ_CP015342
Coordinates   1041321..1043417 (+) Length   698 a.a.
NCBI ID   WP_014843602.1    Uniprot ID   Q6VY32
Organism   Legionella pneumophila strain D-7632     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1036321..1048417
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A5478_RS04725 (A5478_04725) - 1037086..1038093 (-) 1008 WP_014843598.1 hypothetical protein -
  A5478_RS04730 (A5478_04730) pilM 1038510..1039574 (+) 1065 WP_014843599.1 type IV pilus assembly protein PilM Machinery gene
  A5478_RS04735 (A5478_04735) pilN 1039578..1040126 (+) 549 WP_011213354.1 PilN domain-containing protein Machinery gene
  A5478_RS04740 (A5478_04740) pilO 1040140..1040736 (+) 597 WP_027224065.1 type 4a pilus biogenesis protein PilO Machinery gene
  A5478_RS04745 (A5478_04745) pilP 1040733..1041317 (+) 585 WP_014843601.1 pilus assembly protein PilP Machinery gene
  A5478_RS04750 (A5478_04750) pilQ 1041321..1043417 (+) 2097 WP_014843602.1 type IV pilus secretin PilQ Machinery gene
  A5478_RS04755 (A5478_04755) aroK 1043862..1044389 (+) 528 WP_010946667.1 shikimate kinase AroK -
  A5478_RS04760 (A5478_04760) aroB 1044376..1045485 (+) 1110 WP_013101269.1 3-dehydroquinate synthase -
  A5478_RS04765 (A5478_04765) - 1045482..1046933 (+) 1452 WP_027219627.1 SPOR domain-containing protein -
  A5478_RS04770 (A5478_04770) - 1047042..1047473 (+) 432 WP_010946670.1 universal stress protein -

Sequence

Protein


Download         Length: 698 a.a.        Molecular weight: 77441.66 Da        Isoelectric Point: 9.4307

>NTDB_id=178507 A5478_RS04750 WP_014843602.1 1041321..1043417(+) (pilQ) [Legionella pneumophila strain D-7632]
MRKIIAILILISASLSLAYSKDNSLISVKVLPLPENRVRIDFQFSNPLKQLPASFITQKPPRLVLDFISTDMQIPSDQKT
KTIQLGSLHSYNIVAVGDRVRAILELDRSVSYSGSTAGSVYSLVLNGKSNDLFENSKEVFITNQVVNAKHEIKRIDFRGI
EKQGGRVLIDVSDTGIPIDVTQVGKEIVVNFLNTKIPLNLMKRYDVSDFHSPVQIITMQQEGKKVRMTILSKGDYGHFVY
QVNKQFMVDVFPLTAEEIRQAKLKKQVFTGKRISLNFQNISIRAVLQLLADFTGINMVVSDKVQGDITLRLNDIPWDQAL
DIILTTQGLDKRRKGNVMLIDTKASMDKMEEEQLKSQQTIEKLEPIRSDLIQINYAKAADIAVLIKDKQNSLLSDRGKIS
VDARTNTIWIQDTGTKIEEVRELIKQLDIPVKQVLIEARIVEVTKDFSQDIGIRWGVSRPTHLSGTLEGANELARGTAPA
NVVPLERRLNLDLAAAPLTGATPASVGIALAKLNDNILLDLELSALESEGRAELISSPRLITTNQQAAVIESGEEIPYQE
ATSSGATAVAFKKAVLSLKVTPQITPDGKILMDLQINQDTPSPQTFLGVPAIITKEIQTNVLVNNGQTIVLGGIYKQDKN
KVINRIPFFGELPVVGILFSNRQITLKNEELLIFITPRIITNALSITTIEGREKDVYK

Nucleotide


Download         Length: 2097 bp        

>NTDB_id=178507 A5478_RS04750 WP_014843602.1 1041321..1043417(+) (pilQ) [Legionella pneumophila strain D-7632]
GTGAGAAAAATTATCGCGATTTTAATATTAATTAGTGCCAGTTTGAGTCTGGCATATTCTAAAGATAATTCGCTCATTTC
AGTAAAAGTATTGCCATTGCCAGAAAACAGAGTACGTATTGATTTTCAATTTTCCAACCCCTTAAAGCAGTTGCCCGCAA
GCTTTATTACTCAAAAGCCGCCTCGTTTGGTGTTAGATTTTATCAGCACTGATATGCAAATTCCTTCGGATCAGAAAACT
AAAACTATTCAATTAGGCTCATTACACAGTTATAACATTGTTGCTGTTGGTGATAGAGTAAGAGCCATACTGGAACTGGA
TCGTTCGGTGTCCTATTCTGGCAGTACGGCGGGCTCCGTTTATAGCCTTGTCCTGAATGGTAAAAGTAATGATTTATTTG
AAAATAGCAAGGAGGTATTCATAACGAACCAGGTTGTGAACGCCAAGCATGAAATTAAAAGAATTGATTTTCGTGGCATA
GAAAAGCAGGGAGGTAGAGTTTTAATTGATGTTTCTGATACGGGTATTCCAATTGATGTAACTCAAGTTGGGAAAGAAAT
AGTCGTTAATTTCCTGAATACCAAGATCCCTTTGAATTTAATGAAACGGTATGATGTTTCTGATTTTCACAGCCCTGTGC
AGATTATTACGATGCAACAGGAAGGCAAAAAGGTTCGCATGACCATCCTCAGCAAAGGGGATTATGGGCATTTTGTTTAT
CAGGTCAATAAACAATTTATGGTCGATGTCTTTCCTTTAACTGCGGAAGAAATTCGTCAGGCGAAATTGAAGAAACAGGT
ATTTACCGGTAAACGGATTTCTTTGAATTTCCAGAATATTAGTATTCGAGCTGTGCTTCAACTACTGGCAGATTTTACCG
GAATCAATATGGTTGTCAGTGACAAAGTGCAAGGAGATATTACGCTTAGATTAAATGATATCCCCTGGGATCAGGCTCTG
GATATCATTTTAACCACACAAGGGCTAGATAAGCGCAGAAAGGGCAACGTCATGTTGATAGATACCAAAGCCTCCATGGA
TAAAATGGAGGAAGAACAACTCAAAAGTCAACAGACAATTGAAAAACTTGAGCCTATCCGCTCTGATTTAATACAGATTA
ATTATGCAAAGGCAGCAGATATTGCTGTTTTGATCAAGGATAAGCAAAACTCCCTATTATCAGACAGAGGAAAAATAAGT
GTTGATGCCAGAACGAATACAATCTGGATTCAGGATACTGGCACCAAAATTGAGGAAGTAAGGGAACTGATAAAGCAATT
GGATATCCCTGTTAAGCAAGTGCTTATTGAGGCAAGAATTGTTGAAGTAACTAAAGATTTTAGCCAGGATATTGGAATAC
GCTGGGGGGTATCAAGACCAACTCACTTAAGTGGTACACTGGAAGGTGCTAATGAGTTAGCGAGAGGCACAGCACCAGCC
AATGTTGTCCCCTTGGAAAGAAGGTTAAATCTTGATTTGGCAGCTGCTCCTTTAACTGGAGCTACACCGGCCTCGGTAGG
TATTGCGCTAGCTAAACTTAATGATAACATTCTGCTTGATCTGGAGTTGTCTGCTTTGGAAAGCGAAGGGCGTGCTGAAT
TAATATCCAGTCCTCGCCTGATTACTACGAATCAACAGGCAGCAGTCATAGAGTCTGGTGAGGAGATTCCCTATCAGGAG
GCAACATCAAGTGGAGCGACTGCTGTTGCATTTAAAAAAGCAGTATTGAGTTTGAAAGTTACGCCGCAAATTACGCCTGA
TGGTAAAATTTTGATGGATTTACAGATAAATCAGGATACTCCTTCCCCACAAACATTCCTTGGTGTTCCAGCAATAATAA
CAAAGGAGATTCAGACGAATGTTCTGGTTAATAATGGACAAACCATAGTACTTGGAGGTATTTATAAGCAAGATAAGAAC
AAGGTAATCAATAGAATTCCTTTCTTTGGTGAGTTGCCTGTTGTCGGTATTTTATTTAGTAATAGACAAATCACATTGAA
AAATGAAGAATTACTTATTTTTATTACACCAAGAATAATAACGAATGCCTTATCGATTACAACGATAGAGGGACGTGAGA
AAGATGTTTATAAATAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(42-113)

Bacterial type II/III secretion system short domain (Secretin_N)

(370-433)

Bacterial type II and III secretion system protein (Secretin)

(525-680)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q6VY32

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Legionella pneumophila strain ERS1305867

99.713

100

0.997
  pilQ Legionella pneumophila strain Lp02

99.57

100

0.996
  pilQ Pseudomonas aeruginosa PAK

37.872

100

0.383
  comQ Acinetobacter baylyi ADP1

38.719

98.424

0.381
  pilQ Acinetobacter baumannii strain A118

35.949

100

0.361
  pilQ Acinetobacter baumannii D1279779

35.949

100

0.361

Multiple sequence alignment