Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   BE937_RS04250 Genome accession   NZ_CP019020
Coordinates   282449..283942 (+) Length   497 a.a.
NCBI ID   WP_078234969.1    Uniprot ID   -
Organism   Escherichia coli strain Ecol_244     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 277449..288942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BE937_RS04230 (BE937_04375) pilD 278097..278906 (+) 810 WP_000895875.1 prepilin peptidase PppA Machinery gene
  BE937_RS04235 (BE937_04380) gspS2 278972..279382 (+) 411 WP_001298257.1 type II secretion system pilot lipoprotein GspS-beta -
  BE937_RS04240 (BE937_04385) gspC 279400..280359 (+) 960 WP_061157986.1 type II secretion system protein GspC -
  BE937_RS04245 (BE937_04390) gspD 280389..282449 (+) 2061 WP_001314154.1 type II secretion system secretin GspD -
  BE937_RS04250 (BE937_04395) pilB/pilB1 282449..283942 (+) 1494 WP_078234969.1 type II secretion system ATPase GspE Machinery gene
  BE937_RS04255 (BE937_04400) gspF 283942..285165 (+) 1224 WP_061157985.1 type II secretion system inner membrane protein GspF -
  BE937_RS04260 (BE937_04405) gspG 285182..285637 (+) 456 WP_001087289.1 type II secretion system major pseudopilin GspG -
  BE937_RS04265 (BE937_04410) gspH 285641..286204 (+) 564 WP_061157984.1 type II secretion system minor pseudopilin GspH -
  BE937_RS04270 (BE937_04415) gspI 286201..286572 (+) 372 WP_000820077.1 type II secretion system minor pseudopilin GspI -
  BE937_RS04275 (BE937_04420) gspJ 286569..287174 (+) 606 WP_001350472.1 type II secretion system minor pseudopilin GspJ -
  BE937_RS04280 (BE937_04425) gspK 287171..288148 (+) 978 WP_000633183.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55127.28 Da        Isoelectric Point: 5.7687

>NTDB_id=178173 BE937_RS04250 WP_078234969.1 282449..283942(+) (pilB/pilB1) [Escherichia coli strain Ecol_244]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKCVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=178173 BE937_RS04250 WP_078234969.1 282449..283942(+) (pilB/pilB1) [Escherichia coli strain Ecol_244]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTACAGGAGCTACAGCGGGGGCTGA
ACGCGCCTTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAAGCTCGCCAGCTGATGGAAGATCTCGGTTCCGCCGAGGACTTTTTTACCCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGTAAACTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGACTGGATATCGCCGAAAAGTGCGTACC
GCAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTCCGTGTATCAACCATGCCTTCCGCCTGGGGGG
AACGGGTGGTGCTGCGACTGCTGGACAAAAACCAGGCTCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCTCGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACATTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACACGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGATTGCCGCCAGTCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCGAACCGTCACGCTGTACCATCCGCAGGGTTGTCCCGCCTG
TAATCATAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.372

100

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.294

85.513

0.404
  pilB Vibrio parahaemolyticus RIMD 2210633

47.859

79.879

0.382
  pilB Legionella pneumophila strain ERS1305867

43.387

86.72

0.376
  pilB Vibrio campbellii strain DS40M4

46.97

79.678

0.374
  pilB Acinetobacter baumannii D1279779

46.115

80.282

0.37
  pilB Vibrio cholerae strain A1552

45.707

79.678

0.364
  pilB Acinetobacter baylyi ADP1

46.154

78.471

0.362
  pilF Thermus thermophilus HB27

44.75

80.483

0.36