Detailed information NTG0176141

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: AOC10_RS10380
• Genome accession: NZ_CP015018
• Coordinates: 2076279..2077169 (+)
• Length: 296 a.a.
• NCBI ID: WP_011903763.1
• Uniprot ID: A4T076
• Organism: Polynucleobacter asymbioticus strain Tro8F10W22
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2071279..2082169

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AOC10_RS10350 (AOC10_10280)	pth	2071487..2072077 (+)	591	WP_011903757.1	aminoacyl-tRNA hydrolase	-
AOC10_RS10355 (AOC10_10285)	-	2072083..2072349 (-)	267	WP_011903758.1	YfhL family 4Fe-4S dicluster ferredoxin	-
AOC10_RS10360 (AOC10_10290)	coaD	2072367..2072864 (-)	498	WP_011903759.1	pantetheine-phosphate adenylyltransferase	-
AOC10_RS10365 (AOC10_10295)	rsmD	2072901..2073491 (-)	591	WP_011903760.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
AOC10_RS10370 (AOC10_10300)	-	2073511..2074848 (-)	1338	WP_011903761.1	M16 family metallopeptidase	-
AOC10_RS10375 (AOC10_10305)	-	2074848..2076215 (-)	1368	WP_011903762.1	M16 family metallopeptidase	-
AOC10_RS10380 (AOC10_10310)	pilA	2076279..2077169 (+)	891	WP_011903763.1	signal recognition particle-docking protein FtsY	Machinery gene
AOC10_RS10385 (AOC10_10315)	rpoH	2077262..2078191 (+)	930	WP_011903764.1	RNA polymerase sigma factor RpoH	-
AOC10_RS10390 (AOC10_10320)	-	2078213..2078803 (-)	591	WP_011903765.1	SCO family protein	-
AOC10_RS10395 (AOC10_10325)	cyoE	2078805..2079698 (-)	894	WP_011903766.1	heme o synthase	-
AOC10_RS10400 (AOC10_10330)	-	2079695..2080825 (-)	1131	WP_011903767.1	COX15/CtaA family protein	-
AOC10_RS10405 (AOC10_10335)	-	2080825..2081481 (-)	657	WP_011903768.1	hypothetical protein	-

Sequence

Protein

• Length: 296 a.a.
• Molecular weight: 31544.16 Da
• Isoelectric Point: 6.9754

>NTDB_id=176141 AOC10_RS10380 WP_011903763.1 2076279..2077169(+) (pilA) [Polynucleobacter asymbioticus strain Tro8F10W22]
MFGLRKTLGSLFKTSKTDEAWFDTLEESLIQSDVGLPTTEQLIIKLRKAAKSEKASSPEELQALLIQEVSTLLGSLEPNP
NPLFTDQKIHIPEVWLVVGVNGAGKTTTIGKLCKLFQSQGKSVLLAAGDTFRAAARNQLQEWGGRNQVDVITQDGGDAAA
VAHDAIHAANSRKSDILIIDTAGRLATQDHLMEELKKVKRVIGKALPGAPHHTLLVLDGNTGQNGLSQVKAFHAALGLTG
LIVTKLDGTAKGGVICALAHTLKDEPKPAVLALGKGEGIDDLVPFTAHQYSSELFN

Nucleotide

• Length: 891 bp

>NTDB_id=176141 AOC10_RS10380 WP_011903763.1 2076279..2077169(+) (pilA) [Polynucleobacter asymbioticus strain Tro8F10W22]
ATGTTCGGCCTACGTAAAACCCTCGGATCCCTATTCAAAACCAGCAAGACGGATGAAGCTTGGTTTGATACTTTAGAAGA
GTCACTCATTCAAAGTGATGTGGGCCTCCCCACAACTGAACAATTGATTATCAAATTACGTAAAGCTGCAAAATCTGAAA
AAGCATCCAGCCCAGAAGAGCTCCAGGCACTCCTCATACAAGAAGTGAGTACCTTACTAGGTTCACTAGAACCAAATCCA
AACCCATTATTTACAGATCAGAAGATCCATATTCCCGAGGTTTGGTTGGTAGTAGGGGTGAATGGTGCAGGCAAAACCAC
CACTATTGGCAAGTTATGCAAGCTCTTTCAATCTCAAGGTAAATCGGTATTACTGGCAGCTGGCGACACTTTTAGGGCGG
CCGCTAGAAATCAACTTCAAGAATGGGGTGGCAGAAATCAAGTGGATGTCATCACTCAAGATGGTGGTGACGCAGCTGCC
GTAGCCCATGATGCAATTCATGCGGCCAACTCTCGCAAGAGTGACATCTTAATTATTGATACAGCGGGACGACTGGCTAC
CCAAGATCACCTCATGGAAGAGCTCAAAAAAGTGAAGCGGGTGATTGGCAAGGCGCTTCCTGGCGCCCCTCACCACACCC
TACTGGTACTAGATGGCAACACAGGACAGAATGGTTTAAGCCAGGTAAAGGCCTTTCATGCAGCCCTGGGACTTACAGGG
CTCATTGTTACTAAGTTAGATGGCACCGCCAAAGGGGGTGTCATTTGCGCCCTTGCTCACACTCTGAAAGATGAACCCAA
ACCGGCTGTTTTAGCCCTAGGCAAAGGTGAAGGAATTGATGATTTAGTGCCTTTCACAGCCCACCAGTATTCATCTGAAT
TATTCAATTAA

Domains

Predicted by InterproScan.

• SRP54-type protein, helical bundle domain (SRP54_N) (4-68)

• SRP54-type protein, GTPase domain (SRP54) (92-295)

3D structure

• AlphaFold DB: A4T076

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	53.584	98.986	0.53