Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   BSF20_RS13655 Genome accession   NZ_CP018152
Coordinates   2620846..2621850 (+) Length   334 a.a.
NCBI ID   WP_003152685.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain LM2303     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2615846..2626850
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSF20_RS13625 (BSF20_13355) nadA 2616025..2617128 (+) 1104 WP_072588434.1 quinolinate synthase NadA -
  BSF20_RS13630 (BSF20_13360) safA 2617255..2618460 (+) 1206 WP_060387079.1 SafA/ExsA family spore coat assembly protein -
  BSF20_RS13635 (BSF20_13365) - 2618608..2619201 (+) 594 WP_003152677.1 YhcN/YlaJ family sporulation lipoprotein -
  BSF20_RS20885 - 2619335..2619454 (+) 120 WP_024085651.1 hypothetical protein -
  BSF20_RS13645 (BSF20_13375) - 2619586..2620095 (+) 510 WP_012118093.1 intercompartmental signaling factor BofC -
  BSF20_RS13650 (BSF20_13380) ruvA 2620230..2620835 (+) 606 WP_003152683.1 Holliday junction branch migration protein RuvA Machinery gene
  BSF20_RS13655 (BSF20_13385) ruvB 2620846..2621850 (+) 1005 WP_003152685.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  BSF20_RS13660 (BSF20_13390) - 2621843..2622043 (+) 201 WP_043867316.1 DUF2905 domain-containing protein -
  BSF20_RS13665 (BSF20_13395) queA 2622069..2623097 (+) 1029 WP_003152692.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  BSF20_RS13670 (BSF20_13400) tgt 2623125..2624270 (+) 1146 WP_014305500.1 tRNA guanosine(34) transglycosylase Tgt -
  BSF20_RS13675 (BSF20_13405) yajC 2624301..2624561 (+) 261 WP_003152695.1 preprotein translocase subunit YajC -
  BSF20_RS13680 (BSF20_13410) - 2624613..2624999 (-) 387 WP_003152697.1 TIGR04086 family membrane protein -
  BSF20_RS13685 (BSF20_13415) - 2625166..2625822 (+) 657 WP_003152699.1 DUF421 domain-containing protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37225.52 Da        Isoelectric Point: 4.7767

>NTDB_id=173263 BSF20_RS13655 WP_003152685.1 2620846..2621850(+) (ruvB) [Bacillus amyloliquefaciens strain LM2303]
MDERLVSSEADSHESIIEQSLRPQNLAQYIGQKKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE
MRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLNRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVLSRLEYYTQEELTDIVSRTADVFEVDIEEAPALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI
TEEIAHDALERLQVDALGLDHIDHKLLMGMIEKFSGGPVGLDTISATIGEEPHTIEDVYEPYLLQIGFLQRTPRGRVVTP
AVYEHFRLEAPARD

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=173263 BSF20_RS13655 WP_003152685.1 2620846..2621850(+) (ruvB) [Bacillus amyloliquefaciens strain LM2303]
ATGGATGAACGGCTCGTTTCAAGTGAAGCAGACAGCCATGAATCAATAATCGAGCAAAGCCTCAGGCCGCAGAATCTCGC
CCAGTATATCGGCCAAAAGAAGGTGAAAGAAAATCTGCGCGTGTTTATCGATGCGGCGAAAATGAGGCAGGAAACGCTGG
ACCACGTTCTTTTATACGGGCCTCCGGGTCTCGGGAAAACAACGCTTGCGTCTATCGTGGCCAATGAGATGGGTGTGGAG
ATGCGGACGACGTCAGGTCCCGCCATCGAAAGACCGGGAGATTTGGCAGCCATTTTAACGGCGCTCGAACCAGGGGATGT
GCTGTTTATAGACGAAATTCACAGGCTGAACCGCTCAATTGAGGAAGTGCTGTACCCGGCGATGGAGGATTTCTGCCTTG
ATATCGTCATTGGAAAAGGCCCGTCCGCCCGCTCCGTCCGTCTCGATCTTCCCCCTTTTACGCTTGTAGGAGCGACGACA
AGGGTCGGATTATTGACCGCACCGCTCAGAGACCGGTTCGGGGTGCTGTCGCGCCTTGAATACTATACGCAGGAAGAGCT
GACAGATATCGTGTCAAGAACGGCGGACGTTTTTGAAGTGGATATCGAAGAAGCTCCGGCTCTGGAGATCGCCAGACGGT
CAAGGGGAACGCCGCGGGTTGCAAACCGGCTTCTCAGAAGGGTGAGGGATTTTGCCCAGGTGCTCGGCGACAGCCGCATC
ACGGAAGAAATCGCTCACGACGCGCTCGAACGTCTTCAAGTCGATGCGCTCGGCCTTGACCATATCGACCATAAGCTGCT
GATGGGCATGATCGAAAAATTCAGCGGCGGTCCCGTCGGCCTTGATACGATTTCCGCGACAATCGGCGAAGAACCGCATA
CAATAGAGGATGTGTACGAACCGTATCTGCTGCAGATCGGATTTCTCCAGAGGACGCCGAGGGGACGGGTCGTGACCCCG
GCTGTGTATGAACATTTTCGATTGGAGGCGCCCGCCCGTGACTGA

Domains

Predicted by InterProScan.

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)

RuvB AAA lid domain (AAA_lid_4)

(182-255)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

91.916

100

0.919
  ruvB Streptococcus pneumoniae TIGR4

60.736

97.605

0.593
  ruvB Streptococcus pneumoniae R6

60.736

97.605

0.593
  ruvB Streptococcus pneumoniae D39

60.736

97.605

0.593
  ruvB Synechocystis sp. PCC 6803

58.359

98.503

0.575
  ruvB Helicobacter pylori 26695

52.744

98.204

0.518