Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   AYJ52_RS03255 Genome accession   NZ_CP014477
Coordinates   693925..694677 (-) Length   250 a.a.
NCBI ID   WP_017387054.1    Uniprot ID   A0A009HLK6
Organism   Acinetobacter pittii strain AP_882     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 688925..699677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AYJ52_RS03235 (AYJ52_03235) aroB 689467..690549 (-) 1083 WP_017403271.1 3-dehydroquinate synthase -
  AYJ52_RS03240 (AYJ52_03240) aroK 690578..691147 (-) 570 WP_002116987.1 shikimate kinase AroK -
  AYJ52_RS03245 (AYJ52_03245) pilQ 691159..693324 (-) 2166 WP_017387053.1 type IV pilus secretin PilQ Machinery gene
  AYJ52_RS03250 (AYJ52_03250) pilP 693387..693914 (-) 528 WP_002116864.1 pilus assembly protein PilP Machinery gene
  AYJ52_RS03255 (AYJ52_03255) pilO 693925..694677 (-) 753 WP_017387054.1 type 4a pilus biogenesis protein PilO Machinery gene
  AYJ52_RS03260 (AYJ52_03260) pilN 694674..695315 (-) 642 WP_017387055.1 PilN domain-containing protein Machinery gene
  AYJ52_RS03265 (AYJ52_03265) pilM 695315..696373 (-) 1059 WP_005067078.1 pilus assembly protein PilM Machinery gene
  AYJ52_RS03270 (AYJ52_03270) ponA 696535..699096 (+) 2562 WP_017387056.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 250 a.a.        Molecular weight: 28227.07 Da        Isoelectric Point: 4.8782

>NTDB_id=171832 AYJ52_RS03255 WP_017387054.1 693925..694677(-) (pilO) [Acinetobacter pittii strain AP_882]
MSPDELHELSLEQAPSKKKKITLEKFLQQFNTLDMNSYGSWPLSVKITCWIFIFFAVLALGYFVAIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQLQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
ITIEATGDYHAFGAFVSSIAALPRIVTMHDFVVEAAPVKDGKSDIPVLNYSIKAKTYRYMGAVENPEHQEKNASASSVAP
DSSTANTQPK

Nucleotide


Download         Length: 753 bp        

>NTDB_id=171832 AYJ52_RS03255 WP_017387054.1 693925..694677(-) (pilO) [Acinetobacter pittii strain AP_882]
ATGAGTCCTGATGAACTTCATGAGCTGTCTTTAGAACAAGCTCCATCTAAGAAAAAAAAGATCACCTTAGAAAAATTTCT
TCAGCAATTTAACACGCTCGATATGAACAGTTATGGCAGCTGGCCATTATCTGTGAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTATTGGCTTTAGGGTATTTTGTTGCTATTCAACCAAAGCTACAAGCAATTGATAATGCTCAAGCACAAGAA
AGTAATTTATTAAATGAGTTTCGAGAAAAAGACTCAAAATTACGCAACTTGCAGCAATACCAGCTTCAGCTTCAAGAGAT
GCAAGCCAACTTCAATCAGCAGTTAGAACAATTGCCAAAAGAAACCGAAATTCCAAGTTTAGTTGAAGATATTAACTTAA
CGGGGGTGAATTCTGGTCTGAAGTTTAAAAATATCCGCTTAGAAGATGAAGTAAAGCAGGAAATTTTTATTGAACAGCCT
ATTACTATCGAAGCAACTGGGGATTATCACGCTTTCGGTGCTTTTGTTAGCAGTATTGCGGCATTACCACGTATTGTGAC
AATGCATGATTTTGTTGTTGAAGCTGCTCCTGTTAAGGATGGTAAATCAGATATTCCTGTGCTTAATTATTCTATTAAAG
CAAAAACTTATCGCTATATGGGTGCTGTAGAAAATCCTGAACATCAGGAAAAAAATGCTTCGGCTTCCTCGGTAGCACCT
GATTCTTCTACTGCTAACACACAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009HLK6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

83.6

100

0.836
  comO Acinetobacter baylyi ADP1

70

96

0.672

Multiple sequence alignment