Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilY1   Type   Machinery gene
Locus tag   AYJ52_RS03125 Genome accession   NZ_CP014477
Coordinates   661859..665713 (-) Length   1284 a.a.
NCBI ID   WP_017403269.1    Uniprot ID   A0A009I5X3
Organism   Acinetobacter pittii strain AP_882     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 656859..670713
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AYJ52_RS03090 (AYJ52_03090) lip 657599..658540 (+) 942 WP_014207808.1 lipase family alpha/beta hydrolase -
  AYJ52_RS03095 (AYJ52_03095) rplS 658591..658959 (-) 369 WP_002116654.1 50S ribosomal protein L19 -
  AYJ52_RS03100 (AYJ52_03100) trmD 659179..659934 (-) 756 WP_017387036.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  AYJ52_RS03105 (AYJ52_03105) rimM 659977..660525 (-) 549 WP_002116736.1 ribosome maturation factor RimM -
  AYJ52_RS03110 (AYJ52_03110) rpsP 660545..660796 (-) 252 WP_000260334.1 30S ribosomal protein S16 -
  AYJ52_RS03115 (AYJ52_03115) pilE 660944..661369 (-) 426 WP_017387037.1 type IV pilin protein Machinery gene
  AYJ52_RS03120 (AYJ52_03120) pilY2 661366..661848 (-) 483 WP_017387038.1 type IV pilin protein Machinery gene
  AYJ52_RS03125 (AYJ52_03125) pilY1 661859..665713 (-) 3855 WP_017403269.1 PilC/PilY family type IV pilus protein Machinery gene
  AYJ52_RS03130 (AYJ52_03130) pilX 665725..666534 (-) 810 WP_005078460.1 PilX N-terminal domain-containing pilus assembly protein Machinery gene
  AYJ52_RS03135 (AYJ52_03135) pilW 666531..667532 (-) 1002 WP_017387040.1 PilW family protein Machinery gene
  AYJ52_RS03140 (AYJ52_03140) pilV 667532..668092 (-) 561 WP_079381023.1 type IV pilus modification protein PilV Machinery gene
  AYJ52_RS03145 (AYJ52_03145) fimU 668086..668559 (-) 474 WP_017387042.1 pilus assembly FimT family protein Machinery gene
  AYJ52_RS03150 (AYJ52_03150) ispH 668729..669679 (-) 951 WP_017387043.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  AYJ52_RS03155 (AYJ52_03155) gmk 669802..670431 (+) 630 WP_002116973.1 guanylate kinase -

Sequence

Protein


Download         Length: 1284 a.a.        Molecular weight: 137955.40 Da        Isoelectric Point: 7.9851

>NTDB_id=171824 AYJ52_RS03125 WP_017403269.1 661859..665713(-) (pilY1) [Acinetobacter pittii strain AP_882]
MKLKLKNFKPNNLWYAICSSSVAFTWLTTSSVVQASDLQIYASPTAGKKTIVMMLDTSGSMTNNSYGENRLAMLKNGMNA
FLASNNPVLNDTRVGLGNFSANGDSRSGQVLVAAAPLGDASTLNTVGSQRYKLKQAVANLTAGGSTPSAHAYAEAAAYLM
GTTTYSEANYAIRKDSYIRRVRKSDNRTEYSYCTNYRDSQIDTTNLWQPCRSNNYWSNWSTSNPGVDTATAYDTSSDWTY
SYTYYYTTFNYAVANADSGIPKSKSNDTASNPNIVVDRNASNLNAIYQSPLPAVANRQSCDGQGIYFLSDGEPNNTTNTR
SATVMSTALGSTLGANFTCSGGLSNTTADSGWACMGEFAKRLFDKTKNPAGVSIQTAFVGFGSDFSSLSSSDVKNACRLS
SRTQSDRKSDDNCSPNQTTNAVAAPGYGNGGFFPTQSAQGVTDSVIAFINNLDKVPLEPLTTGAISVPYDALNPKNLQEY
GYLRAFEPNPANTYLTWRGNLKKYHVVLSGTNAGAFEANTGGLVYNATGAFRTGTKDYWNSSTYNDGGKVFFGGSYAKVP
LPIAGQNETRDEEGNITKYYYAVQTKIRNLFTDVSAVAADGSLTKISTSGTNLLKIPATPTEGTNPFDSVANTASYVLGK
FNASTGQDILKAFPISLKLKILNYLGYSTDISATTLPSSLVTSNEPYLSMGGSIHSLPVQLTYNGTLDENGNLTSARKQS
ILYGTMEGGLHIVDASTGVEQMAFVPADILNDPVASKALVVGQSDATAPAHGMDGAWVSDPAYSITTTGSGSSAVSKVTA
KQMNIYGGMRMGGSSYYGLNVLNPASPKLLFRVGADQTDYSRMGQSWSKPVLANIRYNGVIKRVMIVGGGYDQCYENPNI
TLSSSCFTNGKAKGNAVYIIDAKTGERLWWTSDTGSNIDNSNMKHSIVSRISTLDRDADGLVDHLYFGDLGGQIFRIDLN
NNQTKTNSTYSSFGIRVVRLANLATNDSTYDASNDYTAGNAPRFYEPPTVTIHDYGIRTFITVGIASGDRSTPLDVYPLT
GREGMSPSTALSGRPVNNVYGIIDKDFIKKNLMSLTDSQLETKDITRSTLRKNPQILRSGETRVAQIFFPNTGAGQNGWY
RSLSSMSDGTEKANNSFRIKGGLKAFEEPMAVTGTLIVPVYDPQGTGIVAADPCLPRVVGETDQQTFCLPFGACLNSDGS
IDQNKENGSGFKTQTGTNCPVGASECNKNVIGSGIRGIAFVPKRDEPPVTNSCGKLQLSGNESGTGEWQCTSHLVPTRWY
ERYR

Nucleotide


Download         Length: 3855 bp        

>NTDB_id=171824 AYJ52_RS03125 WP_017403269.1 661859..665713(-) (pilY1) [Acinetobacter pittii strain AP_882]
GTGAAACTAAAATTGAAGAATTTTAAGCCAAATAATTTATGGTATGCCATCTGTTCGAGTTCAGTTGCTTTTACATGGCT
AACGACAAGCTCCGTTGTGCAGGCGAGTGATTTACAAATTTATGCTTCACCTACAGCTGGTAAAAAAACCATTGTGATGA
TGTTGGATACATCAGGAAGTATGACCAACAATAGTTATGGCGAAAACCGTCTGGCTATGCTTAAAAATGGTATGAATGCT
TTTTTAGCAAGTAATAATCCTGTTTTAAATGACACTCGTGTGGGGTTGGGAAATTTCTCTGCAAATGGTGATAGCCGAAG
TGGGCAGGTTCTAGTGGCTGCTGCTCCTTTAGGAGATGCGAGTACGTTAAATACAGTGGGTTCTCAACGTTATAAATTAA
AACAGGCTGTTGCAAATTTAACTGCTGGCGGGTCAACGCCTTCAGCACATGCTTATGCGGAAGCTGCTGCTTATTTGATG
GGTACAACGACCTATTCAGAGGCAAATTATGCCATTCGAAAAGATAGCTATATAAGGCGTGTGCGAAAATCTGATAATAG
AACGGAATACTCATATTGTACGAATTATCGCGATTCACAAATCGATACTACAAATCTATGGCAACCTTGTCGTTCTAATA
ACTATTGGAGTAACTGGTCAACTTCTAATCCGGGTGTCGATACGGCAACTGCTTATGATACTTCTTCGGATTGGACCTAT
TCTTATACTTATTACTACACCACCTTTAATTATGCTGTAGCAAATGCAGATAGTGGTATCCCAAAATCAAAATCAAATGA
TACAGCTAGCAATCCGAATATTGTTGTAGATAGAAATGCCTCCAATTTAAATGCTATTTACCAATCTCCTTTACCTGCAG
TGGCCAATCGCCAGAGTTGTGATGGGCAAGGAATCTACTTTTTATCAGATGGGGAGCCGAACAATACAACAAATACACGT
TCTGCAACTGTCATGTCGACAGCCCTAGGCAGCACTTTGGGAGCAAATTTTACTTGTTCTGGAGGTTTGTCTAACACGAC
AGCAGACTCTGGTTGGGCCTGTATGGGGGAATTTGCAAAAAGATTATTTGATAAAACAAAAAACCCAGCTGGAGTTTCTA
TTCAAACAGCATTTGTCGGTTTTGGTAGTGACTTTTCTAGCTTAAGTTCTTCTGATGTAAAAAATGCCTGTCGTTTAAGT
TCGAGAACCCAATCTGATCGTAAAAGTGATGATAATTGTTCGCCGAATCAGACCACAAATGCGGTCGCTGCACCTGGTTA
TGGAAATGGAGGCTTTTTCCCTACCCAAAGTGCGCAAGGTGTAACAGATAGTGTTATCGCATTTATTAATAACTTAGATA
AAGTTCCTTTAGAACCCTTAACCACAGGTGCTATCTCTGTTCCTTATGATGCACTAAATCCTAAAAATTTACAGGAATAT
GGTTACTTACGTGCTTTTGAGCCAAACCCGGCTAACACCTACTTAACATGGCGTGGAAATTTAAAGAAATATCATGTTGT
TTTATCGGGAACAAATGCTGGTGCTTTTGAAGCGAATACTGGTGGGTTGGTTTACAACGCAACTGGGGCATTTAGAACGG
GTACGAAAGATTATTGGAATAGCTCTACTTATAATGATGGAGGTAAAGTCTTTTTTGGTGGTTCATATGCAAAAGTTCCT
TTACCAATTGCTGGACAAAATGAAACACGTGATGAAGAAGGAAATATCACAAAATATTATTACGCAGTACAAACTAAAAT
CCGCAACTTATTTACTGATGTTTCCGCTGTTGCGGCGGATGGTAGCTTAACGAAAATATCGACTTCTGGAACTAATTTGC
TAAAAATTCCAGCTACTCCAACAGAAGGAACTAATCCTTTTGATAGTGTGGCTAATACAGCAAGTTATGTTTTAGGAAAA
TTTAATGCATCAACTGGACAAGATATTTTAAAAGCTTTCCCGATTAGCTTGAAATTAAAAATATTAAATTATCTAGGTTA
CTCAACTGATATTAGTGCTACGACCTTACCTTCGTCATTAGTTACATCGAATGAACCCTATTTATCGATGGGCGGTAGTA
TTCATTCTTTACCGGTACAGCTTACCTATAATGGAACATTAGATGAAAATGGGAATTTAACTTCTGCGCGTAAACAATCC
ATTCTCTATGGAACGATGGAAGGCGGTCTGCATATTGTGGATGCCTCAACTGGTGTAGAACAAATGGCTTTCGTTCCTGC
CGATATATTAAATGATCCAGTTGCATCAAAAGCTTTAGTGGTTGGCCAAAGTGATGCTACAGCTCCTGCTCACGGTATGG
ATGGAGCTTGGGTATCTGACCCTGCATATAGTATTACTACAACGGGAAGCGGTAGCTCAGCAGTATCAAAAGTAACTGCA
AAGCAAATGAATATTTATGGTGGCATGCGAATGGGGGGCAGCAGCTATTATGGCTTAAATGTCTTAAATCCTGCATCACC
TAAGCTCCTGTTTAGAGTAGGAGCAGATCAAACTGATTATAGTCGCATGGGGCAGAGTTGGTCTAAACCTGTACTTGCAA
ATATACGTTATAACGGGGTGATTAAACGCGTTATGATTGTTGGTGGTGGCTACGACCAATGTTATGAAAACCCGAATATT
ACCTTGAGTAGTTCTTGCTTTACGAATGGAAAAGCAAAAGGGAATGCTGTTTATATTATTGATGCTAAAACTGGGGAACG
CTTATGGTGGACTAGTGATACAGGCTCTAATATAGATAACTCTAATATGAAGCACAGTATTGTTAGTCGCATTAGTACCT
TGGATCGAGATGCTGATGGTTTAGTCGACCATTTATATTTTGGTGATTTAGGCGGGCAAATCTTCCGTATCGATCTTAAT
AATAACCAGACAAAAACAAATTCAACTTATAGCAGTTTCGGTATTCGAGTTGTTCGATTAGCGAATTTAGCAACAAATGA
CTCAACTTATGATGCTTCGAATGACTATACCGCTGGGAATGCGCCGCGTTTTTATGAACCGCCAACAGTGACAATTCACG
ATTATGGAATCCGCACTTTTATTACTGTCGGTATTGCTTCAGGTGACCGTAGTACACCTTTAGATGTTTATCCGCTTACA
GGTCGTGAGGGGATGTCACCGAGCACCGCATTAAGTGGAAGACCTGTAAACAATGTTTATGGAATTATCGACAAAGACTT
TATTAAAAAGAATTTAATGTCTTTAACAGATAGCCAGCTTGAAACAAAAGATATTACTCGATCGACGCTTCGAAAAAATC
CACAAATTTTACGATCTGGAGAAACACGAGTAGCACAGATTTTCTTTCCCAACACTGGGGCGGGACAAAACGGTTGGTAT
CGCTCACTTTCTAGTATGAGCGATGGCACAGAGAAAGCTAATAATAGTTTCCGTATTAAAGGTGGACTGAAAGCTTTTGA
AGAACCTATGGCCGTTACTGGGACTTTAATTGTGCCTGTTTATGATCCGCAGGGAACAGGCATTGTAGCGGCAGACCCAT
GTTTACCCCGTGTAGTAGGTGAAACTGACCAGCAGACGTTCTGTTTACCATTTGGCGCCTGCCTTAATTCTGATGGATCA
ATTGATCAGAATAAAGAGAATGGAAGTGGATTTAAAACACAAACTGGTACTAATTGCCCAGTAGGAGCTTCTGAATGTAA
TAAAAACGTTATTGGCTCCGGTATTCGCGGCATAGCATTTGTTCCAAAAAGAGATGAACCTCCAGTGACTAATAGTTGTG
GGAAATTACAACTGTCTGGAAACGAGAGTGGAACGGGCGAGTGGCAGTGTACAAGTCATTTAGTTCCTACACGCTGGTAT
GAGCGTTATCGCTAA

Domains

Predicted by InterproScan.

von Willebrand factor type A domain (VWA_2)

(51-161)

Neisseria PilC beta-propeller domain (T4P_PilY1)

(714-965)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilY1 Acinetobacter baumannii D1279779

85.039

100

0.854

Multiple sequence alignment