Detailed information NTG0169853

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AXF37_RS04715
• Genome accession: NZ_CP014257
• Coordinates: 1029228..1030292 (+)
• Length: 354 a.a.
• NCBI ID: WP_027221264.1
• Uniprot ID: A0AAX2IUM9
• Organism: Legionella pneumophila subsp. pascullei strain D-7119
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1024228..1035292

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AXF37_RS04705 (AXF37_04705)	-	1025261..1027645 (-)	2385	WP_027221262.1	penicillin-binding protein 1A	-
AXF37_RS04710 (AXF37_04710)	-	1027796..1028809 (-)	1014	WP_027221263.1	hypothetical protein	-
AXF37_RS04715 (AXF37_04715)	pilM	1029228..1030292 (+)	1065	WP_027221264.1	pilus assembly protein PilM	Machinery gene
AXF37_RS04720 (AXF37_04720)	pilN	1030296..1030844 (+)	549	WP_027221265.1	PilN domain-containing protein	Machinery gene
AXF37_RS04725 (AXF37_04725)	pilO	1030858..1031457 (+)	600	WP_027221266.1	type 4a pilus biogenesis protein PilO	Machinery gene
AXF37_RS04730 (AXF37_04730)	pilP	1031454..1032038 (+)	585	WP_027221267.1	pilus assembly protein PilP	Machinery gene
AXF37_RS04735 (AXF37_04735)	pilQ	1032042..1034138 (+)	2097	WP_027221268.1	type IV pilus secretin PilQ	Machinery gene
AXF37_RS04740 (AXF37_04740)	aroK	1034581..1035108 (+)	528	WP_032828804.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38794.80 Da
• Isoelectric Point: 5.0331

>NTDB_id=169853 AXF37_RS04715 WP_027221264.1 1029228..1030292(+) (pilM) [Legionella pneumophila subsp. pascullei strain D-7119]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEQVCVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDQEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKNSALLDVLIVASRAENVNSRVEAAHR
AGLEPKIVDVESYAVERSVQQIAQELPAGGQDKIIAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSYMSFGKKVNLDAINNDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=169853 AXF37_RS04715 WP_027221264.1 1029228..1030292(+) (pilM) [Legionella pneumophila subsp. pascullei strain D-7119]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATTAGTTCTTCAGCAGTGAAAATTTTGGAAAT
TTCAGGTTCCGGAGAGCAAGTTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCTCTTGAAGGGAATATGA
TCAAGGACGTCGATGTAGTAGCGAATTGTATCAAAAAATTGGTAGAAAGGTTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATTAGCAAAGTAGTGCAAATTAATGAAGGATTGACTGACCAGGAAATGGAAGAGCTTGT
CGTTATTGAAGCGGATAAGTATATTCCTTACCCAATAGACGAAATAAATCTTGATTTTGAAGTGTTAGGTCATTCTGCCA
AGAATTCAGCGCTACTTGATGTTTTAATCGTGGCCTCAAGGGCAGAAAATGTTAATAGTCGTGTTGAAGCAGCTCATAGA
GCTGGCTTGGAGCCTAAAATCGTTGACGTTGAATCTTATGCAGTAGAACGTTCAGTGCAACAAATTGCTCAAGAATTGCC
AGCAGGTGGACAAGACAAAATTATAGCGATAGTAGATATTGGAGCCAATTACACTCATTTATTTGTTTTGCATGGCATGC
GATTAATCTTTTCAAGAGAAGAAAAATTTGGTGGCATGCAGCTTGTTGAAGCTATTGCAGAACATTATCATATGAATTTA
GAGCAGGCTATTGCCCTAAAGAATCAGGGTAAATTACCAGAGGACTATGAATCTGCTGTATTAGAGCCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGCACTTTGCAGTTTTTTTATTCTACCAGTCAACATGGATTTGTAGACCATATTTTATTAG
CTGGAGGAATGGCAAGGCAACCAGGGTTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTAGCAAATCCG
CTATCCTATATGTCTTTTGGTAAAAAAGTGAATTTGGATGCCATTAATAATGACGCGCCTACACTGATGGTGGCTTGTGG
GTTAGCTCTAAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	96.61	100	0.966
comM	Acinetobacter nosocomialis M2	43.22	100	0.432
pilM	Acinetobacter baumannii D1279779	42.938	100	0.429
comM	Acinetobacter baylyi ADP1	40.96	100	0.41