Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VN23_RS10300 Genome accession   NZ_CP014222
Coordinates   2292869..2294041 (-) Length   390 a.a.
NCBI ID   WP_046351189.1    Uniprot ID   A0A0X8GLM5
Organism   Janthinobacterium sp. B9-8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2287869..2299041
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VN23_RS10270 (VN23_10270) kdsB 2287892..2288662 (-) 771 WP_046351195.1 3-deoxy-manno-octulosonate cytidylyltransferase -
  VN23_RS10275 (VN23_10275) - 2288659..2288841 (-) 183 WP_046351194.1 Trm112 family protein -
  VN23_RS10280 (VN23_10280) lpxK 2288858..2289862 (-) 1005 WP_046351193.1 tetraacyldisaccharide 4'-kinase -
  VN23_RS10285 (VN23_10285) - 2289896..2290318 (-) 423 WP_046351192.1 biopolymer transporter ExbD -
  VN23_RS10290 (VN23_10290) - 2290328..2290936 (-) 609 WP_046351191.1 MotA/TolQ/ExbB proton channel family protein -
  VN23_RS10295 (VN23_10295) xseA 2291444..2292799 (+) 1356 WP_046351190.1 exodeoxyribonuclease VII large subunit -
  VN23_RS10300 (VN23_10300) pilU 2292869..2294041 (-) 1173 WP_046351189.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VN23_RS10305 (VN23_10305) - 2294402..2295331 (+) 930 WP_046351188.1 Nudix family hydrolase -
  VN23_RS10310 (VN23_10310) - 2295335..2295760 (+) 426 WP_052746510.1 hypothetical protein -
  VN23_RS10315 (VN23_10315) - 2295767..2296270 (+) 504 WP_046351187.1 YgjP-like metallopeptidase domain-containing protein -
  VN23_RS10320 (VN23_10320) cysG 2296368..2297750 (-) 1383 WP_046351186.1 siroheme synthase CysG -
  VN23_RS10325 (VN23_10325) - 2297936..2298238 (+) 303 WP_046351185.1 c-type cytochrome -

Sequence

Protein


Download         Length: 390 a.a.        Molecular weight: 43567.08 Da        Isoelectric Point: 5.9471

>NTDB_id=169547 VN23_RS10300 WP_046351189.1 2292869..2294041(-) (pilU) [Janthinobacterium sp. B9-8]
MAPEQASKTINDLLRMMLQHKASDLFIADDFPPAMKINGRLTPVGNQKLNGVISKALAYSIMRPDQVEEFERDYECNFAI
NPEGIGRFRVNVFMQQEKVGMVLRVITSKIPDFDELKLPPILKDVVLAKRGLVLLVGGTGSGKSTTMAAMIDYRNQNTHG
HIITIEDPIEYVHKSKNCLVTHREVGRDTKHWFAALKNTLRQAPDVILIGEIRDRDTMEYALSFAETGHLCMGTLHANSA
NQALERIVNFFPEERHPQIYMDLSLNMRGMVSQRLIPTPDGKGRVAAIEILLNSPLVADMIFKGDVGGVKTAMLKSEELG
MQTFDSALFHLYEDGRISLEEALKNADSINELRLRVKLKSKDSTSVSHDELGHLSLVAEAEPEEDEGKAE

Nucleotide


Download         Length: 1173 bp        

>NTDB_id=169547 VN23_RS10300 WP_046351189.1 2292869..2294041(-) (pilU) [Janthinobacterium sp. B9-8]
ATGGCTCCAGAACAAGCCAGTAAAACAATCAATGATTTGCTGCGTATGATGTTGCAGCATAAAGCGTCTGATCTTTTTAT
TGCTGATGATTTTCCACCTGCGATGAAAATCAATGGCAGGTTAACACCGGTTGGAAATCAAAAATTAAATGGTGTGATTT
CCAAGGCTTTGGCCTATTCAATCATGCGGCCCGATCAGGTTGAAGAATTTGAGCGGGATTACGAGTGCAATTTTGCTATT
AACCCCGAAGGTATCGGTCGCTTTCGGGTTAATGTGTTTATGCAGCAAGAAAAAGTCGGCATGGTACTGCGGGTTATTAC
CAGCAAAATTCCCGATTTTGATGAGTTGAAATTACCGCCCATTTTAAAAGACGTGGTGCTGGCTAAGCGTGGCTTGGTGC
TGCTAGTGGGCGGCACTGGCTCGGGTAAATCGACCACGATGGCGGCAATGATTGATTATCGTAATCAAAATACTCACGGC
CATATCATTACCATTGAAGACCCGATTGAATATGTGCATAAAAGCAAAAATTGTTTAGTGACTCACCGTGAAGTAGGGCG
AGATACCAAGCATTGGTTTGCAGCACTAAAAAACACCTTAAGGCAAGCGCCCGATGTGATTTTAATTGGCGAGATTCGCG
ATCGGGACACCATGGAATATGCTCTTTCTTTTGCCGAAACAGGGCACTTATGTATGGGCACCTTACATGCCAACAGCGCT
AATCAGGCTTTGGAGCGAATTGTTAATTTCTTCCCTGAAGAGCGCCATCCGCAAATTTATATGGATTTATCATTGAATAT
GCGCGGCATGGTTTCTCAGCGTTTGATTCCAACGCCAGATGGAAAAGGGCGGGTTGCAGCGATTGAAATTTTGCTTAATT
CACCTTTAGTGGCGGATATGATTTTTAAAGGTGATGTAGGCGGTGTGAAAACGGCCATGCTGAAGTCTGAAGAATTAGGC
ATGCAAACTTTTGATTCGGCGCTGTTTCATTTATACGAAGATGGCCGGATTAGCCTTGAAGAAGCGCTTAAAAATGCCGA
TTCGATTAATGAATTACGCTTGCGGGTTAAATTAAAAAGTAAAGACAGTACCAGCGTTTCTCATGATGAATTAGGGCATT
TAAGCTTGGTGGCTGAAGCAGAGCCTGAAGAAGATGAGGGCAAGGCCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0X8GLM5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.944

92.308

0.526
  pilU Acinetobacter baylyi ADP1

57.143

89.744

0.513
  pilU Vibrio cholerae strain A1552

53.824

90.513

0.487
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.323

86.41

0.374
  pilT Pseudomonas aeruginosa PAK

43.027

86.41

0.372

Multiple sequence alignment