Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   RSJ22_RS20390 Genome accession   NZ_CP014174
Coordinates   4012203..4012649 (-) Length   148 a.a.
NCBI ID   WP_003359348.1    Uniprot ID   A0A0D1BNS4
Organism   Clostridium botulinum strain B609     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context

Location: 4007203..4017649
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RSJ22_RS20365 (RSJ22_20135) rplI 4008032..4008475 (-) 444 WP_012048442.1 50S ribosomal protein L9 -
  RSJ22_RS20370 (RSJ22_20140) - 4008475..4010460 (-) 1986 WP_003397329.1 DHH family phosphoesterase -
  RSJ22_RS20375 (RSJ22_20145) - 4010461..4011465 (-) 1005 WP_045541137.1 YybS family protein -
  RSJ22_RS20380 (RSJ22_20150) - 4011477..4011794 (-) 318 WP_003359330.1 MazG-like family protein -
  RSJ22_RS20385 (RSJ22_20155) rpsR 4011942..4012184 (-) 243 WP_003359407.1 30S ribosomal protein S18 -
  RSJ22_RS20390 (RSJ22_20160) ssbA 4012203..4012649 (-) 447 WP_003359348.1 single-stranded DNA-binding protein Machinery gene
  RSJ22_RS20395 (RSJ22_20165) rpsF 4012665..4012949 (-) 285 WP_003359427.1 30S ribosomal protein S6 -
  RSJ22_RS20400 (RSJ22_20170) - 4013055..4014203 (-) 1149 WP_003359361.1 double-cubane-cluster-containing anaerobic reductase -
  RSJ22_RS20405 (RSJ22_20175) - 4014477..4014674 (-) 198 WP_003398916.1 DUF951 domain-containing protein -
  RSJ22_RS20410 (RSJ22_20180) - 4014690..4015559 (-) 870 WP_085321153.1 mechanosensitive ion channel family protein -
  RSJ22_RS20415 (RSJ22_20185) - 4015566..4015811 (-) 246 WP_003361815.1 DUF3343 domain-containing protein -
  RSJ22_RS20420 (RSJ22_20190) - 4016002..4017156 (+) 1155 WP_012048446.1 aminotransferase class V-fold PLP-dependent enzyme -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 16325.15 Da        Isoelectric Point: 4.7821

>NTDB_id=169239 RSJ22_RS20390 WP_003359348.1 4012203..4012649(-) (ssbA) [Clostridium botulinum strain B609]
MNKVVLIGRLTKDPELKFTPGNGTAVATFTLAVDRRFSKDGQREADFIPIVVWGKQAESTANYMSKGKLMGVSGRIQTRS
YEAKDGTRRYVTEVIAEEVKFLEWGNKPTTDSTYNNGSEPSYQSGQGFGNGNSFDDDIIPVDDGDIPF

Nucleotide


Download         Length: 447 bp        

>NTDB_id=169239 RSJ22_RS20390 WP_003359348.1 4012203..4012649(-) (ssbA) [Clostridium botulinum strain B609]
ATGAATAAAGTTGTTTTGATAGGTAGATTAACCAAAGATCCTGAGTTAAAATTTACTCCAGGAAACGGGACAGCAGTTGC
CACCTTTACTTTGGCTGTAGATAGAAGATTTTCAAAAGATGGTCAGAGAGAAGCAGATTTCATCCCTATAGTAGTATGGG
GAAAACAAGCAGAATCTACAGCTAACTACATGAGTAAAGGAAAACTTATGGGGGTTAGTGGTAGAATTCAAACAAGAAGC
TATGAAGCTAAAGATGGTACAAGAAGATATGTAACAGAAGTCATAGCTGAAGAAGTTAAATTTCTTGAGTGGGGCAATAA
GCCTACAACGGATTCAACATATAATAATGGGTCAGAACCAAGTTATCAATCAGGCCAAGGCTTTGGAAATGGAAACAGTT
TTGACGACGATATAATTCCAGTGGATGATGGAGACATCCCATTTTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(1-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1BNS4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

48.837

100

0.568
  ssb Latilactobacillus sakei subsp. sakei 23K

46.269

90.541

0.419
  ssb Neisseria meningitidis MC58

33.526

100

0.392
  ssbB Bacillus subtilis subsp. subtilis str. 168

55.34

69.595

0.385
  ssbB Streptococcus sobrinus strain NIDR 6715-7

53.398

69.595

0.372

Multiple sequence alignment