Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   AV541_RS03820 Genome accession   NZ_CP014141
Coordinates   669310..669891 (-) Length   193 a.a.
NCBI ID   WP_060384271.1    Uniprot ID   A0A0X8D7F8
Organism   Thermus parvatiensis strain RL     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 664310..674891
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AV541_RS03800 (AV541_03805) - 664404..664958 (-) 555 WP_060384268.1 shikimate kinase -
  AV541_RS03805 (AV541_03810) aroC 664983..666134 (-) 1152 WP_060384269.1 chorismate synthase -
  AV541_RS03810 (AV541_03815) pilQ 666193..668466 (-) 2274 WP_060384270.1 secretin N-terminal domain-containing protein Machinery gene
  AV541_RS13515 pilW 668463..668720 (-) 258 WP_244880586.1 hypothetical protein Machinery gene
  AV541_RS03815 (AV541_03820) pilW 668717..669313 (-) 597 WP_244880587.1 hypothetical protein Machinery gene
  AV541_RS03820 (AV541_03825) pilO 669310..669891 (-) 582 WP_060384271.1 type 4a pilus biogenesis protein PilO Machinery gene
  AV541_RS03825 (AV541_03830) pilN 669881..670504 (-) 624 WP_060384272.1 hypothetical protein Machinery gene
  AV541_RS03830 (AV541_03835) pilM 670497..671630 (-) 1134 WP_060384273.1 type IV pilus assembly protein PilM Machinery gene
  AV541_RS03835 (AV541_03840) - 671761..672765 (-) 1005 WP_060384274.1 homoisocitrate dehydrogenase -
  AV541_RS03840 (AV541_03845) - 672820..673311 (-) 492 WP_060384275.1 DUF5317 family protein -
  AV541_RS03845 (AV541_03850) - 673362..674813 (-) 1452 WP_060384276.1 HD-GYP domain-containing protein -

Sequence

Protein


Download         Length: 193 a.a.        Molecular weight: 21442.78 Da        Isoelectric Point: 6.4681

>NTDB_id=168673 AV541_RS03820 WP_060384271.1 669310..669891(-) (pilO) [Thermus parvatiensis strain RL]
MLARLGQREWALLAMVLTALLGLLWYYLFIVPTRQEIATVRQEIDRLTIERNRGLQARRALPELRATIAALQAQRLAFLR
ALPREERLAEVLSEVLQDAEASGVVVRSFTRSRTSAPVPEVRAVNLALALEAPFPEIYAYLRRLEDLSRFSTLSGLNLSV
QSQEANPTLATGLTLTVYVLAQGVEEETGGSTP

Nucleotide


Download         Length: 582 bp        

>NTDB_id=168673 AV541_RS03820 WP_060384271.1 669310..669891(-) (pilO) [Thermus parvatiensis strain RL]
GTGCTCGCTAGGCTGGGACAGCGGGAGTGGGCCCTCCTGGCCATGGTCCTCACCGCCCTCCTGGGGCTCCTCTGGTACTA
CCTATTCATCGTGCCCACGAGGCAGGAGATCGCCACCGTCCGGCAGGAGATTGACCGCCTCACCATAGAGCGCAACCGCG
GGCTCCAGGCCCGCCGGGCCCTTCCCGAGCTCCGCGCCACCATCGCCGCCCTTCAGGCCCAAAGGCTCGCCTTCCTCCGG
GCCCTGCCCCGGGAGGAAAGGCTCGCCGAGGTCCTCTCCGAGGTGCTCCAGGACGCGGAGGCGAGCGGGGTGGTGGTCCG
AAGCTTCACCCGAAGCCGCACCTCGGCCCCGGTGCCCGAGGTGCGGGCCGTGAACCTGGCCCTGGCCCTCGAGGCCCCCT
TTCCCGAAATCTACGCCTATCTGCGGCGCCTGGAGGACCTCTCCCGCTTCAGCACCCTTTCCGGCCTCAACCTGAGCGTC
CAGAGCCAGGAAGCGAACCCCACCCTCGCCACCGGCCTCACCTTGACCGTCTACGTGCTGGCCCAGGGGGTGGAGGAGGA
AACGGGAGGGAGCACCCCATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(41-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0X8D7F8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Thermus thermophilus HB27

97.927

100

0.979

Multiple sequence alignment