Detailed information NTG0168675

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AV541_RS03830
• Genome accession: NZ_CP014141
• Coordinates: 670497..671630 (-)
• Length: 377 a.a.
• NCBI ID: WP_060384273.1
• Uniprot ID: A0A109QIE5
• Organism: Thermus parvatiensis strain RL
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 665497..676630

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AV541_RS03810 (AV541_03815)	pilQ	666193..668466 (-)	2274	WP_060384270.1	secretin N-terminal domain-containing protein	Machinery gene
AV541_RS13515	pilW	668463..668720 (-)	258	WP_244880586.1	hypothetical protein	Machinery gene
AV541_RS03815 (AV541_03820)	pilW	668717..669313 (-)	597	WP_244880587.1	hypothetical protein	Machinery gene
AV541_RS03820 (AV541_03825)	pilO	669310..669891 (-)	582	WP_060384271.1	type 4a pilus biogenesis protein PilO	Machinery gene
AV541_RS03825 (AV541_03830)	pilN	669881..670504 (-)	624	WP_060384272.1	hypothetical protein	Machinery gene
AV541_RS03830 (AV541_03835)	pilM	670497..671630 (-)	1134	WP_060384273.1	type IV pilus assembly protein PilM	Machinery gene
AV541_RS03835 (AV541_03840)	-	671761..672765 (-)	1005	WP_060384274.1	homoisocitrate dehydrogenase	-
AV541_RS03840 (AV541_03845)	-	672820..673311 (-)	492	WP_060384275.1	DUF5317 family protein	-
AV541_RS03845 (AV541_03850)	-	673362..674813 (-)	1452	WP_060384276.1	HD-GYP domain-containing protein	-
AV541_RS03860 (AV541_03865)	-	675555..675920 (+)	366	WP_060384277.1	hypothetical protein	-

Sequence

Protein

• Length: 377 a.a.
• Molecular weight: 41441.08 Da
• Isoelectric Point: 4.3805

>NTDB_id=168675 AV541_RS03830 WP_060384273.1 670497..671630(-) (pilM) [Thermus parvatiensis strain RL]
MFKSLSQLFRPRVEALGLEIGASALKLVEVSGNPPALKALASRPTPPGLLVEGMVAEPAALAQEIKELLLEARTRKRYVV
TALSNLAVILRPIQVPKMPLKEMEEAVRWEAERYIPFPIDEVVLDFAPLTPLSEVQEGEQVQVMVAAARQEAVAGVLEAL
RGAGLVPVVLDVKPFAGLYPLEARLAEEPDRVFLVLDIGAESTSLVLLRGDKPLAVRVLTLSGKDFTEAIARSFNLDLLA
AEEVKRTYGMATLPTEDEELLLDFDAEWERYSPGRIYDAIRPVLVELTQELRRSLEFFRIQLEEAPPEVGYLLGGGSKLR
GLASLLTDTLGVNFEPVNPWEAVTVDPKRFEAEKLQEMGPEFAVALGLALRGVEPFD

Nucleotide

• Length: 1134 bp

>NTDB_id=168675 AV541_RS03830 WP_060384273.1 670497..671630(-) (pilM) [Thermus parvatiensis strain RL]
GTGTTCAAAAGCCTTAGCCAGCTCTTCCGACCCAGGGTTGAGGCCCTGGGGTTGGAGATCGGGGCCTCCGCCCTGAAGCT
CGTGGAGGTGTCCGGGAACCCCCCTGCCCTCAAGGCCCTGGCCTCCCGCCCCACCCCGCCCGGCCTCCTGGTGGAAGGGA
TGGTGGCCGAGCCCGCCGCGTTGGCCCAGGAGATCAAGGAGCTTCTCCTCGAGGCCCGCACCCGCAAGCGCTACGTGGTC
ACCGCCCTCTCCAACCTGGCGGTCATCCTCCGCCCCATCCAGGTTCCCAAGATGCCCCTCAAGGAGATGGAGGAGGCGGT
GCGCTGGGAGGCGGAGCGCTACATCCCCTTCCCCATTGACGAGGTGGTCCTGGACTTCGCCCCCCTGACCCCCCTCTCCG
AGGTGCAGGAGGGGGAGCAGGTCCAGGTGATGGTGGCGGCGGCGCGGCAGGAGGCGGTGGCGGGGGTCCTCGAGGCCTTG
AGGGGGGCGGGGCTCGTCCCCGTGGTGCTGGACGTGAAGCCCTTCGCCGGGCTCTACCCTCTGGAGGCCAGGCTTGCGGA
GGAGCCCGACCGGGTCTTCCTCGTCCTGGACATCGGGGCCGAGAGCACGAGCCTCGTCCTGCTCCGGGGGGACAAGCCCC
TGGCGGTGCGCGTCCTCACCCTCTCGGGCAAGGACTTCACCGAGGCCATCGCCCGAAGCTTCAACCTGGACCTCCTCGCC
GCCGAGGAGGTGAAGCGGACCTACGGGATGGCCACCCTCCCCACCGAGGACGAGGAGCTCCTCCTGGACTTTGACGCCGA
GTGGGAGCGCTACAGCCCGGGGCGCATCTATGACGCCATCCGCCCGGTGCTGGTGGAGCTCACCCAGGAGCTCCGCCGTA
GCCTGGAGTTCTTCCGCATCCAGCTGGAAGAAGCCCCGCCCGAGGTGGGCTACCTCCTGGGCGGGGGAAGCAAGTTGAGG
GGGCTTGCCTCCCTCCTCACCGACACCCTGGGGGTGAACTTTGAGCCCGTCAATCCCTGGGAGGCGGTAACGGTGGACCC
CAAGCGCTTTGAGGCGGAGAAGCTCCAGGAGATGGGGCCCGAGTTCGCCGTAGCCCTGGGCCTGGCCCTGAGGGGGGTGG
AGCCCTTTGATTAG

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (17-256)

• Type IV pilus assembly protein PilM; (PilM_2) (280-372)

3D structure

• AlphaFold DB: A0A109QIE5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Thermus thermophilus HB27	97.613	100	0.976