Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   AL483_RS22855 Genome accession   NZ_CP014016
Coordinates   2055038..2056666 (-) Length   542 a.a.
NCBI ID   WP_061054988.1    Uniprot ID   A0AAE5HUS2
Organism   Staphylococcus simulans strain FDAARGOS_124     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2044593..2065369 2055038..2056666 within 0


Gene organization within MGE regions


Location: 2044593..2065369
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL483_RS22785 (AL483_09990) - 2044650..2044898 (+) 249 Protein_1937 type I restriction enzyme endonuclease domain-containing protein -
  AL483_RS22790 (AL483_09995) - 2045088..2045597 (-) 510 WP_061054977.1 DUF1643 domain-containing protein -
  AL483_RS22795 (AL483_10000) - 2045615..2045926 (-) 312 WP_061054978.1 DUF960 family protein -
  AL483_RS22800 (AL483_10005) - 2046011..2046361 (-) 351 WP_061054979.1 SAUGI family uracil-DNA glycosylase inhibitor -
  AL483_RS25460 - 2046885..2047028 (+) 144 WP_158257850.1 hypothetical protein -
  AL483_RS22805 (AL483_12655) - 2047010..2047174 (+) 165 WP_226956313.1 hypothetical protein -
  AL483_RS22810 (AL483_10010) - 2047654..2048148 (-) 495 WP_061054980.1 GNAT family N-acetyltransferase -
  AL483_RS22815 (AL483_10015) - 2048404..2049780 (-) 1377 WP_061054981.1 amino acid permease -
  AL483_RS22825 (AL483_10025) - 2050559..2050780 (-) 222 WP_061054983.1 hypothetical protein -
  AL483_RS22830 (AL483_10030) - 2050794..2051291 (-) 498 WP_061054984.1 DUF1643 domain-containing protein -
  AL483_RS22835 (AL483_10035) - 2051307..2051618 (-) 312 WP_061054985.1 DUF960 family protein -
  AL483_RS22840 (AL483_10040) - 2051705..2052055 (-) 351 WP_061054986.1 SAUGI family uracil-DNA glycosylase inhibitor -
  AL483_RS22845 (AL483_10045) - 2052692..2054026 (+) 1335 WP_002479755.1 IS1182 family transposase -
  AL483_RS22850 (AL483_10050) - 2054216..2054950 (-) 735 WP_061054987.1 glucose 1-dehydrogenase -
  AL483_RS22855 (AL483_10055) ccrB 2055038..2056666 (-) 1629 WP_061054988.1 cassette chromosome recombinase CcrB Machinery gene
  AL483_RS22860 (AL483_10060) ccrA 2056702..2058036 (-) 1335 WP_061054989.1 cassette chromosome recombinase CcrA Machinery gene
  AL483_RS22865 (AL483_10065) cch1 2058224..2059993 (-) 1770 WP_061054990.1 cassette chromosome replicative helicase -
  AL483_RS22870 (AL483_10070) ssb 2059993..2060283 (-) 291 WP_061054991.1 cassette chromosome ssDNA-binding protein -
  AL483_RS25605 - 2060465..2061259 (+) 795 WP_237449266.1 hypothetical protein -
  AL483_RS25610 - 2061490..2061645 (+) 156 WP_237449267.1 hypothetical protein -
  AL483_RS25615 - 2061724..2062068 (+) 345 WP_237449268.1 hypothetical protein -
  AL483_RS22880 (AL483_10080) - 2062122..2062490 (+) 369 WP_237449269.1 hypothetical protein -
  AL483_RS22885 (AL483_10085) - 2062518..2063192 (-) 675 WP_001105999.1 IS6-like element IS257 family transposase -
  AL483_RS22890 (AL483_12660) - 2063382..2063905 (+) 524 Protein_1960 transposase -
  AL483_RS22900 (AL483_10095) - 2064272..2064946 (+) 675 WP_001105999.1 IS6-like element IS257 family transposase -
  AL483_RS22905 (AL483_10100) - 2065007..2065369 (-) 363 Protein_1962 recombinase family protein -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62820.63 Da        Isoelectric Point: 9.6699

>NTDB_id=167252 AL483_RS22855 WP_061054988.1 2055038..2056666(-) (ccrB) [Staphylococcus simulans strain FDAARGOS_124]
MQKLKQKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCNFHHFEVKDIYDDRGISGKSMNRPELQRLLKDANEGHIDCVM
VYKTNRLARNTSDLLKIVEDLHRQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPDSKHELMINQHEANIVKYIFESYAKGHGYRKMANALNHKGYVTKKGNPFSTSAIAYILTNPFYIGKIQFAK
YKDWNEKRRKGLNDNPIIADGKHSPIISQELWDKVHSRMKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEKYVMDQILEIVKSDKVINQVLERVNQENKVDIGALNHDIAYKQQQYD
EIHAKLDNLIKTIEDNPDLTTILKETIHKYETQLNDITNQINQLKQQQNQEKTSYNTKQLSAVLRRIFQNVESMDKSQLK
ALYLTVIDRIDIRKDEHRKKQFYVTLKLNHEITKQLFHNTPIDEVRLSTSSLFLSQTLYIHI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=167252 AL483_RS22855 WP_061054988.1 2055038..2056666(-) (ccrB) [Staphylococcus simulans strain FDAARGOS_124]
ATGCAAAAACTCAAACAAAAACGTGTCGGTATCTATGTTCGTGTATCAACAGAAATGCAAAGTACTGAAGGATATAGTAT
CGACGGACAAATCAATCAAATCAAAGAATATTGTAACTTTCATCATTTCGAGGTCAAAGATATATACGACGATCGTGGTA
TATCAGGAAAGTCTATGAACCGACCAGAACTACAACGTTTGTTAAAAGATGCGAACGAAGGTCATATCGATTGTGTCATG
GTATATAAAACGAATAGACTCGCACGTAATACATCAGACTTACTGAAAATAGTTGAAGACCTTCATCGTCAAAATGTCGA
ATTCTTCAGCTTATCTGAACGTATGGAAGTCAACACTTCTTCGGGTAAACTCATGTTACAAATACTTGCAAGCTTTTCTG
AATTTGAAAGAAACAATATTGTCGAGAACGTATTCATGGGACAAACCCGACGTGCTCAAGAAGGCTATTATCAAGGCAAT
TTGCCGCTGGGCTATGACAAAATACCGGATAGCAAGCATGAACTCATGATAAATCAACATGAAGCGAATATCGTCAAATA
TATCTTTGAGTCATATGCTAAAGGTCATGGTTATCGAAAAATGGCTAACGCACTTAATCATAAAGGTTATGTAACAAAGA
AAGGTAATCCATTTAGTACATCAGCTATAGCTTATATCCTTACCAACCCCTTCTACATCGGTAAAATTCAATTTGCGAAG
TATAAAGACTGGAATGAGAAACGACGTAAAGGTCTTAATGACAATCCTATAATAGCTGACGGGAAGCATTCACCTATCAT
TAGTCAAGAACTATGGGATAAAGTACATTCACGTATGAAGCAAGTTAGTCAAAAACCCCAAGTTCATGGAAAAGGAACCA
ACCTATTAACAGGTATTATTCACTGCCCACAATGTGGTGCACCAATGGCAGCTAGTAACACAACGAACACATTGAAAGAT
GGTACCAAGAAGCGAATACGCTATTATTCTTGTAGTAACTTTCGCAACAAGGGGTCAAAAGTATGTTCTGCGAATAGTGT
TAGAGCTGATGTGATTGAGAAATACGTCATGGATCAAATACTCGAAATTGTCAAAAGTGATAAAGTCATTAACCAAGTCT
TAGAACGTGTCAATCAAGAGAATAAAGTCGATATTGGTGCATTGAACCATGATATCGCTTATAAACAACAACAATACGAT
GAAATCCATGCGAAACTAGACAACTTGATTAAAACAATCGAAGATAATCCAGACTTAACAACAATACTCAAAGAAACAAT
CCATAAATATGAAACACAACTGAATGACATTACGAATCAAATTAATCAACTGAAGCAACAACAAAATCAAGAGAAAACGT
CATATAATACGAAACAACTATCCGCTGTTTTACGTCGCATATTTCAAAACGTAGAATCCATGGATAAATCACAACTCAAA
GCGTTATATCTCACAGTCATTGACCGAATAGATATTCGTAAAGACGAACATCGTAAAAAGCAATTCTATGTCACATTAAA
ACTCAATCATGAAATCACTAAACAACTCTTCCATAATACCCCTATCGACGAAGTGCGCCTCAGCACTTCGTCTTTATTTT
TATCTCAAACACTCTATATTCATATATAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(11-156)

Recombinase (Recombinase)

(180-281)

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

84.502

100

0.845

Multiple sequence alignment