Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   RSJ14_RS19970 Genome accession   NZ_CP013847
Coordinates   4100204..4100650 (-) Length   148 a.a.
NCBI ID   WP_003359348.1    Uniprot ID   A0A0D1BNS4
Organism   Clostridium botulinum strain Af650     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context

Location: 4095204..4105650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RSJ14_RS19945 (RSJ14_19720) rplI 4096033..4096476 (-) 444 WP_003359455.1 50S ribosomal protein L9 -
  RSJ14_RS19950 (RSJ14_19725) - 4096476..4098461 (-) 1986 WP_012705477.1 DHH family phosphoesterase -
  RSJ14_RS19955 (RSJ14_19730) - 4098462..4099466 (-) 1005 WP_012704806.1 YybS family protein -
  RSJ14_RS19960 (RSJ14_19735) - 4099478..4099795 (-) 318 WP_003359330.1 MazG-like family protein -
  RSJ14_RS19965 (RSJ14_19740) rpsR 4099943..4100185 (-) 243 WP_003359407.1 30S ribosomal protein S18 -
  RSJ14_RS19970 (RSJ14_19745) ssbA 4100204..4100650 (-) 447 WP_003359348.1 single-stranded DNA-binding protein Machinery gene
  RSJ14_RS19975 (RSJ14_19750) rpsF 4100666..4100950 (-) 285 WP_003359427.1 30S ribosomal protein S6 -
  RSJ14_RS19980 (RSJ14_19755) - 4101056..4102204 (-) 1149 WP_003359361.1 double-cubane-cluster-containing anaerobic reductase -
  RSJ14_RS19985 (RSJ14_19760) - 4102478..4102675 (-) 198 WP_003359459.1 DUF951 domain-containing protein -
  RSJ14_RS19990 (RSJ14_19765) - 4102691..4103560 (-) 870 WP_003359339.1 mechanosensitive ion channel family protein -
  RSJ14_RS19995 (RSJ14_19770) - 4103567..4103812 (-) 246 WP_003359413.1 DUF3343 domain-containing protein -
  RSJ14_RS20000 (RSJ14_19775) - 4104003..4105157 (+) 1155 WP_003359382.1 aminotransferase class V-fold PLP-dependent enzyme -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 16325.15 Da        Isoelectric Point: 4.7821

>NTDB_id=165228 RSJ14_RS19970 WP_003359348.1 4100204..4100650(-) (ssbA) [Clostridium botulinum strain Af650]
MNKVVLIGRLTKDPELKFTPGNGTAVATFTLAVDRRFSKDGQREADFIPIVVWGKQAESTANYMSKGKLMGVSGRIQTRS
YEAKDGTRRYVTEVIAEEVKFLEWGNKPTTDSTYNNGSEPSYQSGQGFGNGNSFDDDIIPVDDGDIPF

Nucleotide


Download         Length: 447 bp        

>NTDB_id=165228 RSJ14_RS19970 WP_003359348.1 4100204..4100650(-) (ssbA) [Clostridium botulinum strain Af650]
ATGAATAAAGTTGTTTTGATAGGTAGATTAACCAAAGATCCTGAGTTAAAATTTACTCCAGGAAACGGGACAGCAGTTGC
CACCTTTACTTTGGCTGTAGATAGAAGATTTTCAAAAGATGGTCAGAGAGAAGCAGATTTCATCCCTATAGTAGTATGGG
GAAAACAAGCAGAATCTACAGCTAACTACATGAGTAAAGGAAAACTTATGGGGGTTAGTGGTAGAATTCAAACAAGAAGC
TATGAAGCTAAAGATGGTACAAGAAGATATGTAACAGAAGTCATAGCTGAAGAAGTTAAATTTCTTGAGTGGGGCAATAA
GCCTACAACGGATTCAACATATAATAATGGGTCAGAACCAAGTTATCAATCAGGCCAAGGCTTTGGAAATGGAAACAGTT
TTGACGACGATATAATTCCAGTGGATGATGGAGACATCCCATTTTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(1-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1BNS4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

48.837

100

0.568
  ssb Latilactobacillus sakei subsp. sakei 23K

46.269

90.541

0.419
  ssb Neisseria meningitidis MC58

33.526

100

0.392
  ssbB Bacillus subtilis subsp. subtilis str. 168

55.34

69.595

0.385
  ssbB Streptococcus sobrinus strain NIDR 6715-7

53.398

69.595

0.372

Multiple sequence alignment