Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   RSJ2_RS18565 Genome accession   NZ_CP013707
Coordinates   3830743..3831189 (-) Length   148 a.a.
NCBI ID   WP_003359348.1    Uniprot ID   A0A0D1BNS4
Organism   Clostridium botulinum strain F634     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context


Location: 3825743..3836189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RSJ2_RS18540 (RSJ2_3601) rplI 3826572..3827015 (-) 444 WP_003359455.1 50S ribosomal protein L9 -
  RSJ2_RS18545 (RSJ2_3602) - 3827015..3829000 (-) 1986 WP_003361808.1 DHH family phosphoesterase -
  RSJ2_RS18550 (RSJ2_3603) - 3829001..3830005 (-) 1005 WP_003361809.1 YybS family protein -
  RSJ2_RS18555 (RSJ2_3604) - 3830017..3830334 (-) 318 WP_003359330.1 MazG-like family protein -
  RSJ2_RS18560 (RSJ2_3605) rpsR 3830482..3830724 (-) 243 WP_003359407.1 30S ribosomal protein S18 -
  RSJ2_RS18565 (RSJ2_3606) ssbA 3830743..3831189 (-) 447 WP_003359348.1 single-stranded DNA-binding protein Machinery gene
  RSJ2_RS18570 (RSJ2_3607) rpsF 3831205..3831489 (-) 285 WP_003361811.1 30S ribosomal protein S6 -
  RSJ2_RS18575 (RSJ2_3608) - 3831595..3832743 (-) 1149 WP_024932354.1 double-cubane-cluster-containing anaerobic reductase -
  RSJ2_RS18580 (RSJ2_3609) - 3833009..3833206 (-) 198 WP_003361813.1 DUF951 domain-containing protein -
  RSJ2_RS18585 (RSJ2_3610) - 3833222..3834091 (-) 870 WP_003361814.1 mechanosensitive ion channel family protein -
  RSJ2_RS18590 (RSJ2_3611) - 3834098..3834343 (-) 246 WP_003361815.1 DUF3343 domain-containing protein -
  RSJ2_RS18595 (RSJ2_3612) - 3834534..3835688 (+) 1155 WP_021135937.1 aminotransferase class V-fold PLP-dependent enzyme -

Sequence


Protein


Download         Length: 148 a.a.        Molecular weight: 16325.15 Da        Isoelectric Point: 4.7821

>NTDB_id=164244 RSJ2_RS18565 WP_003359348.1 3830743..3831189(-) (ssbA) [Clostridium botulinum strain F634]
MNKVVLIGRLTKDPELKFTPGNGTAVATFTLAVDRRFSKDGQREADFIPIVVWGKQAESTANYMSKGKLMGVSGRIQTRS
YEAKDGTRRYVTEVIAEEVKFLEWGNKPTTDSTYNNGSEPSYQSGQGFGNGNSFDDDIIPVDDGDIPF

Nucleotide


Download         Length: 447 bp        

>NTDB_id=164244 RSJ2_RS18565 WP_003359348.1 3830743..3831189(-) (ssbA) [Clostridium botulinum strain F634]
ATGAATAAAGTTGTTTTGATAGGTAGATTAACCAAAGATCCTGAGTTAAAATTTACTCCAGGAAACGGGACAGCAGTTGC
CACCTTTACTTTGGCTGTAGATAGAAGATTTTCAAAAGATGGTCAGAGAGAAGCAGATTTCATCCCTATAGTAGTATGGG
GAAAACAAGCAGAATCTACAGCTAACTACATGAGTAAAGGAAAACTTATGGGGGTTAGTGGTAGAATTCAAACAAGAAGC
TATGAAGCTAAAGATGGTACAAGAAGATATGTAACAGAAGTCATAGCTGAAGAAGTTAAATTTCTTGAGTGGGGCAATAA
GCCTACAACGGATTCAACATATAATAATGGGTCAGAACCAAGTTATCAATCAGGCCAAGGCTTTGGAAATGGAAACAGTT
TTGACGACGATATAATTCCAGTGGATGATGGAGACATCCCATTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0D1BNS4

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

48.837

100

0.568

  ssb Latilactobacillus sakei subsp. sakei 23K

46.269

90.541

0.419

  ssb Neisseria meningitidis MC58

33.526

100

0.392

  ssbB Bacillus subtilis subsp. subtilis str. 168

55.34

69.595

0.385

  ssbB Streptococcus sobrinus strain NIDR 6715-7

53.398

69.595

0.372


Multiple sequence alignment