Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   NPD1_RS07530 Genome accession   NZ_CP013296
Coordinates   1252489..1252935 (-) Length   148 a.a.
NCBI ID   WP_003359348.1    Uniprot ID   A0A0D1BNS4
Organism   Clostridium botulinum strain CDC_54064     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context

Location: 1247489..1257935
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPD1_RS07505 (NPD1_1254) rplI 1248318..1248761 (-) 444 WP_003359455.1 50S ribosomal protein L9 -
  NPD1_RS07510 (NPD1_1255) - 1248761..1250746 (-) 1986 WP_012705477.1 DHH family phosphoesterase -
  NPD1_RS07515 (NPD1_1256) - 1250747..1251751 (-) 1005 WP_021107300.1 YybS family protein -
  NPD1_RS07520 (NPD1_1257) - 1251763..1252080 (-) 318 WP_003359330.1 MazG-like family protein -
  NPD1_RS07525 (NPD1_1258) rpsR 1252228..1252470 (-) 243 WP_003359407.1 30S ribosomal protein S18 -
  NPD1_RS07530 (NPD1_1259) ssbA 1252489..1252935 (-) 447 WP_003359348.1 single-stranded DNA-binding protein Machinery gene
  NPD1_RS07535 (NPD1_1260) rpsF 1252951..1253235 (-) 285 WP_003359427.1 30S ribosomal protein S6 -
  NPD1_RS07540 (NPD1_1261) - 1253341..1254489 (-) 1149 WP_003359361.1 double-cubane-cluster-containing anaerobic reductase -
  NPD1_RS07545 (NPD1_1262) - 1254763..1254960 (-) 198 WP_003359459.1 DUF951 domain-containing protein -
  NPD1_RS07550 (NPD1_1263) - 1254976..1255845 (-) 870 WP_003359339.1 mechanosensitive ion channel family protein -
  NPD1_RS07555 (NPD1_1264) - 1255852..1256097 (-) 246 WP_003359413.1 DUF3343 domain-containing protein -
  NPD1_RS07560 (NPD1_1265) - 1256288..1257442 (+) 1155 WP_003359382.1 aminotransferase class V-fold PLP-dependent enzyme -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 16325.15 Da        Isoelectric Point: 4.7821

>NTDB_id=161590 NPD1_RS07530 WP_003359348.1 1252489..1252935(-) (ssbA) [Clostridium botulinum strain CDC_54064]
MNKVVLIGRLTKDPELKFTPGNGTAVATFTLAVDRRFSKDGQREADFIPIVVWGKQAESTANYMSKGKLMGVSGRIQTRS
YEAKDGTRRYVTEVIAEEVKFLEWGNKPTTDSTYNNGSEPSYQSGQGFGNGNSFDDDIIPVDDGDIPF

Nucleotide


Download         Length: 447 bp        

>NTDB_id=161590 NPD1_RS07530 WP_003359348.1 1252489..1252935(-) (ssbA) [Clostridium botulinum strain CDC_54064]
ATGAATAAAGTTGTTTTGATAGGTAGATTAACCAAAGATCCTGAGTTAAAATTTACTCCAGGAAACGGGACAGCAGTTGC
CACCTTTACTTTGGCTGTAGATAGAAGATTTTCAAAAGATGGTCAGAGAGAAGCAGATTTCATCCCTATAGTAGTATGGG
GAAAACAAGCAGAATCTACAGCTAACTACATGAGTAAAGGAAAACTTATGGGGGTTAGTGGTAGAATTCAAACAAGAAGC
TATGAAGCTAAAGATGGTACAAGAAGATATGTAACAGAAGTCATAGCTGAAGAAGTTAAATTTCTTGAGTGGGGCAATAA
GCCTACAACGGATTCAACATATAATAATGGGTCAGAACCAAGTTATCAATCAGGCCAAGGCTTTGGAAATGGAAACAGTT
TTGACGACGATATAATTCCAGTGGATGATGGAGACATCCCATTTTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(1-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1BNS4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

48.837

100

0.568
  ssb Latilactobacillus sakei subsp. sakei 23K

46.269

90.541

0.419
  ssb Neisseria meningitidis MC58

33.526

100

0.392
  ssbB Bacillus subtilis subsp. subtilis str. 168

55.34

69.595

0.385
  ssbB Streptococcus sobrinus strain NIDR 6715-7

53.398

69.595

0.372

Multiple sequence alignment