Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   NPD3_RS12240 Genome accession   NZ_CP013247
Coordinates   2545936..2546382 (-) Length   148 a.a.
NCBI ID   WP_003359348.1    Uniprot ID   A0A0D1BNS4
Organism   Clostridium botulinum strain CDC_69094     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context

Location: 2540936..2551382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPD3_RS12215 (NPD3_2370) rplI 2541765..2542208 (-) 444 WP_003359455.1 50S ribosomal protein L9 -
  NPD3_RS12220 (NPD3_2371) - 2542208..2544193 (-) 1986 WP_061319725.1 DHH family phosphoesterase -
  NPD3_RS12225 (NPD3_2372) - 2544194..2545198 (-) 1005 WP_003398913.1 YybS family protein -
  NPD3_RS12230 (NPD3_2373) - 2545210..2545527 (-) 318 WP_061319726.1 MazG-like family protein -
  NPD3_RS12235 (NPD3_2374) rpsR 2545675..2545917 (-) 243 WP_003359407.1 30S ribosomal protein S18 -
  NPD3_RS12240 (NPD3_2375) ssbA 2545936..2546382 (-) 447 WP_003359348.1 single-stranded DNA-binding protein Machinery gene
  NPD3_RS12245 (NPD3_2376) rpsF 2546398..2546682 (-) 285 WP_003359427.1 30S ribosomal protein S6 -
  NPD3_RS12250 (NPD3_2377) - 2546788..2547936 (-) 1149 WP_061319727.1 double-cubane-cluster-containing anaerobic reductase -
  NPD3_RS12255 (NPD3_2378) - 2548210..2548407 (-) 198 WP_003398916.1 DUF951 domain-containing protein -
  NPD3_RS12260 (NPD3_2379) - 2548423..2549292 (-) 870 WP_061319728.1 mechanosensitive ion channel family protein -
  NPD3_RS12265 (NPD3_2380) - 2549299..2549544 (-) 246 WP_003361815.1 DUF3343 domain-containing protein -
  NPD3_RS12270 (NPD3_2381) - 2549735..2550889 (+) 1155 WP_061319729.1 aminotransferase class V-fold PLP-dependent enzyme -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 16325.15 Da        Isoelectric Point: 4.7821

>NTDB_id=161162 NPD3_RS12240 WP_003359348.1 2545936..2546382(-) (ssbA) [Clostridium botulinum strain CDC_69094]
MNKVVLIGRLTKDPELKFTPGNGTAVATFTLAVDRRFSKDGQREADFIPIVVWGKQAESTANYMSKGKLMGVSGRIQTRS
YEAKDGTRRYVTEVIAEEVKFLEWGNKPTTDSTYNNGSEPSYQSGQGFGNGNSFDDDIIPVDDGDIPF

Nucleotide


Download         Length: 447 bp        

>NTDB_id=161162 NPD3_RS12240 WP_003359348.1 2545936..2546382(-) (ssbA) [Clostridium botulinum strain CDC_69094]
ATGAATAAAGTTGTTTTGATAGGTAGATTAACCAAAGATCCTGAGTTAAAATTTACTCCAGGAAACGGGACAGCAGTTGC
CACCTTTACTTTGGCTGTAGATAGAAGATTTTCAAAAGATGGTCAGAGAGAAGCAGATTTCATCCCTATAGTAGTATGGG
GAAAACAAGCAGAATCTACAGCTAACTACATGAGTAAAGGAAAACTTATGGGGGTTAGTGGTAGAATTCAAACAAGAAGC
TATGAAGCTAAAGATGGTACAAGAAGATATGTAACAGAAGTCATAGCTGAAGAAGTTAAATTTCTTGAGTGGGGCAATAA
GCCTACAACGGATTCAACATATAATAATGGGTCAGAACCAAGTTATCAATCAGGCCAAGGCTTTGGAAATGGAAACAGTT
TTGACGACGATATAATTCCAGTGGATGATGGAGACATCCCATTTTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(1-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1BNS4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

48.837

100

0.568
  ssb Latilactobacillus sakei subsp. sakei 23K

46.269

90.541

0.419
  ssb Neisseria meningitidis MC58

33.526

100

0.392
  ssbB Bacillus subtilis subsp. subtilis str. 168

55.34

69.595

0.385
  ssbB Streptococcus sobrinus strain NIDR 6715-7

53.398

69.595

0.372

Multiple sequence alignment