Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   APY30_RS17025 Genome accession   NZ_CP013006
Coordinates   3758596..3760332 (-) Length   578 a.a.
NCBI ID   WP_033836748.1    Uniprot ID   A0AA44Z4L9
Organism   Xanthomonas citri pv. malvacearum strain XcmN1003     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3753596..3765332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  APY30_RS17010 (APY30_16245) - 3754630..3756243 (+) 1614 WP_005921369.1 HAMP domain-containing sensor histidine kinase -
  APY30_RS17015 (APY30_16255) pilR 3756510..3757964 (+) 1455 WP_033836745.1 sigma-54 dependent transcriptional regulator Regulator
  APY30_RS17020 (APY30_16260) - 3758151..3758318 (-) 168 WP_033836746.1 hypothetical protein -
  APY30_RS17025 (APY30_16265) pilB 3758596..3760332 (-) 1737 WP_033836748.1 type IV-A pilus assembly ATPase PilB Machinery gene
  APY30_RS17030 pilA2 3760374..3760787 (-) 414 WP_005921365.1 pilin Machinery gene
  APY30_RS17035 (APY30_16270) comP 3760884..3761312 (-) 429 WP_005921364.1 pilin Machinery gene
  APY30_RS17040 (APY30_16275) pilC 3761657..3762916 (+) 1260 WP_033836749.1 type II secretion system F family protein Machinery gene
  APY30_RS17045 (APY30_16280) - 3762923..3763786 (+) 864 WP_003491180.1 A24 family peptidase -
  APY30_RS17050 (APY30_16285) coaE 3763800..3764408 (+) 609 WP_005915836.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62690.83 Da        Isoelectric Point: 5.9527

>NTDB_id=159123 APY30_RS17025 WP_033836748.1 3758596..3760332(-) (pilB) [Xanthomonas citri pv. malvacearum strain XcmN1003]
MNVAIGSNLVGITGIARRLVQDGALDEASARAAMDHATQAKVPLPQWFAEKKLVTAAQLAAANAVEFGMPLMDVSAFDAN
QNAVKLVSEELLQKHQVLPLFKRGNRLFIGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASFGSSLG
NDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVKKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=159123 APY30_RS17025 WP_033836748.1 3758596..3760332(-) (pilB) [Xanthomonas citri pv. malvacearum strain XcmN1003]
ATGAACGTTGCAATCGGCTCAAATCTAGTTGGAATTACTGGTATTGCGCGGCGCCTTGTTCAGGATGGTGCGCTTGATGA
GGCGAGCGCGCGTGCGGCAATGGATCACGCAACCCAGGCCAAAGTTCCTTTACCGCAATGGTTCGCCGAAAAGAAGCTTG
TAACAGCCGCACAACTCGCGGCCGCCAACGCAGTCGAGTTTGGCATGCCTTTGATGGATGTATCGGCGTTCGATGCCAAC
CAGAACGCGGTCAAGCTCGTCAGTGAGGAGCTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCATAGGGGTGAGCAACCCGACACAGACTCGCGCGCTGGACGACATCAAGTTCCATACGAACTTAGTGGTCGAGGCAA
TCCTCGTCGACGAAGATCAGATCCGCCGGACGCTCGAGCAGTGGCAGGCCAGCAATGCGTCGTTTGGTTCTTCGCTTGGC
AACGACGACGAGGAAATGGGGGATCTGGACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAAAAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGACGGCTTGTTGAAGAACGTGGCGAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTTCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAAA
AAGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTCCTGGAGGCGATCCACAAGCCGTACGGGATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTACACCGCGCTGGGCATCCTCAACGACGAGACACGCAATATCTCCACTGCGGAGGACCCGGTTGAAATCCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGTGGCATGACCTTCGCCGCGGCTTTGCGCTCGTTCCTGCGACAGGATCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGTCACATGGT
GCTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCACGTCTGATGAACATGGGCATTGCGCCCTACAACATCA
CCTCGTCGGTGACGCTGGTGATCGCGCAGCGTCTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCATTGCTGGCCGAAGGGTTCACGCCTGCACAGCTTGCCGCCGGGATCGAGCTGTATGAAGCGGTCGGTTGCGA
TGAGTGCACCGAAGGTTACAAGGGGCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCGCAATCGTGC
TGGAAGGCGGGAACGCGATGCAGATCGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCGTTGATG
AAGGCTGCGCACGGGGTGACCAGCCTGGCCGAGATCAATCGTGTAACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.81

98.27

0.548
  pilB Acinetobacter baylyi ADP1

54.93

98.27

0.54
  pilB Legionella pneumophila strain ERS1305867

51.384

100

0.514
  pilB Vibrio cholerae strain A1552

48.616

100

0.486
  pilF Neisseria gonorrhoeae MS11

48.592

98.27

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

46.25

96.886

0.448
  pilB Vibrio campbellii strain DS40M4

44.991

98.443

0.443

Multiple sequence alignment