Detailed information NTG0156173

Bioinformatically predicted

Overview

• Name: pknB
• Type: Regulator
• Locus tag: LLUC77_RS10685
• Genome accession: NZ_CP015906
• Coordinates: 2079903..2081786 (-)
• Length: 627 a.a.
• NCBI ID: WP_003130801.1
• Uniprot ID: Q9CEF5
• Organism: Lactococcus lactis subsp. lactis strain UC77
• Function: modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology) Competence regulation

Genomic Context

Location: 2074903..2086786

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLUC77_RS10655 (LLUC77_2038)	macP	2074941..2075219 (-)	279	WP_003130796.1	cell wall synthase accessory phosphoprotein MacP	-
LLUC77_RS10660 (LLUC77_2039)	-	2075212..2075796 (-)	585	WP_010906182.1	NUDIX hydrolase	-
LLUC77_RS10665 (LLUC77_2040)	glmU	2075901..2077277 (-)	1377	WP_003130798.1	bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU	-
LLUC77_RS10670 (LLUC77_2041)	proC	2077505..2078293 (+)	789	WP_010906183.1	pyrroline-5-carboxylate reductase	-
LLUC77_RS10675 (LLUC77_2042)	-	2078368..2079315 (-)	948	WP_003130410.1	IS30 family transposase	-
LLUC77_RS10680 (LLUC77_2043)	rpsO	2079452..2079721 (-)	270	WP_010906184.1	30S ribosomal protein S15	-
LLUC77_RS10685 (LLUC77_2044)	pknB	2079903..2081786 (-)	1884	WP_003130801.1	Stk1 family PASTA domain-containing Ser/Thr kinase	Regulator
LLUC77_RS10690 (LLUC77_2045)	-	2081786..2082562 (-)	777	WP_003130802.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
LLUC77_RS10695 (LLUC77_2046)	rsmB	2082643..2083917 (-)	1275	WP_010906185.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
LLUC77_RS10700 (LLUC77_2047)	-	2084116..2084622 (-)	507	WP_003131479.1	Asp23/Gls24 family envelope stress response protein	-
LLUC77_RS10705 (LLUC77_2048)	-	2084643..2084831 (-)	189	WP_012898445.1	DUF2273 domain-containing protein	-
LLUC77_RS10710 (LLUC77_2049)	amaP	2084842..2085402 (-)	561	WP_010906186.1	alkaline shock response membrane anchor protein AmaP	-
LLUC77_RS10715 (LLUC77_2050)	-	2085430..2085672 (-)	243	WP_003131476.1	GlsB/YeaQ/YmgE family stress response membrane protein	-

Sequence

Protein

• Length: 627 a.a.
• Molecular weight: 67877.92 Da
• Isoelectric Point: 4.7783

>NTDB_id=156173 LLUC77_RS10685 WP_003130801.1 2079903..2081786(-) (pknB) [Lactococcus lactis subsp. lactis strain UC77]
MIQIGKIFADRYRIIKEIGRGGMANVYQGEDTFLGDRLVAIKVLRSNFENDDIAIARFQREAFAMAELSHPNIVGISDVG
EFESQQYIVMEFVDGMTLKQYINQNAPLANDEAIEIITEILSAMDMAHSHGIIHRDLKPQNVLVSSSGTVKVTDFGIAKA
LSETSLTQTNTMFGSVHYLSPEQARGSNATVQSDIYAIGIILFELLTGQIPFDGDSAVAIALKHFQESIPSIINLNPEVP
QALENVVIKATAKDIKNRYTDVEEMMTDVATSTSLDRRGEEKLVFNKDHDETKIMPANLINPYDTKPLIDKKEDNDSQTD
EKAASSEVGNKNKKSKKGLIIGLIILLLVVGGATLAWVVSTPTNVKIPNVTNSTLSQAKSKIKDAKLKVGTVHKQQSSTI
AEGKVIKTDPTSGTTVRSNSSVDIYVSTGNEDIIKMKDFVGEKIDEAMATLLKDYGIDESQVTQTSVPSDSYPAGTIIKQ
SPKKGSSFDTKGSEKITFEVSSGKQVEVPDYKPNGQYMTYSQYQAALKAAGFTNITLDPQATTNQQADGYVYSVYPNVGT
SVDPTQEIVVTYSVYTAPSSSSTTSESTTTSETSSSTTSSTSSSTTSQPSTDNNNSSKESSTTSSSS

Nucleotide

• Length: 1884 bp

>NTDB_id=156173 LLUC77_RS10685 WP_003130801.1 2079903..2081786(-) (pknB) [Lactococcus lactis subsp. lactis strain UC77]
ATGATTCAAATCGGTAAAATCTTTGCCGATCGTTATCGGATTATCAAAGAAATCGGACGTGGCGGAATGGCGAACGTTTA
TCAAGGTGAAGATACTTTTCTTGGTGACCGCTTAGTTGCAATAAAAGTGCTACGTTCAAATTTTGAAAACGATGATATCG
CGATTGCTCGTTTTCAGCGAGAAGCATTCGCCATGGCAGAACTTTCACATCCAAATATCGTAGGTATTTCTGATGTTGGT
GAATTTGAAAGTCAACAATACATTGTCATGGAATTTGTTGACGGCATGACACTTAAACAATACATCAATCAAAATGCACC
TTTAGCCAATGATGAAGCTATTGAAATTATTACTGAAATTTTATCAGCAATGGACATGGCCCATTCACATGGTATTATTC
ACCGGGATTTAAAACCACAAAATGTTTTAGTTTCCAGTAGTGGAACTGTAAAAGTAACGGATTTTGGAATTGCGAAAGCA
CTCTCTGAAACTTCACTTACTCAAACAAATACAATGTTTGGTTCGGTTCATTATTTATCACCAGAGCAAGCGCGTGGTTC
AAATGCAACTGTTCAATCAGATATTTATGCGATTGGAATTATTTTATTTGAGCTTTTAACAGGTCAAATTCCATTTGATG
GAGATTCTGCAGTTGCTATCGCTCTAAAACATTTCCAAGAGAGTATTCCAAGCATCATTAATCTCAATCCAGAAGTTCCG
CAAGCTTTGGAAAATGTTGTCATTAAAGCTACAGCGAAAGATATTAAAAATCGCTATACTGATGTAGAAGAAATGATGAC
TGATGTGGCAACTTCAACGAGTCTTGACCGTCGTGGGGAAGAAAAATTAGTTTTCAATAAGGATCATGATGAAACAAAAA
TTATGCCTGCCAATCTAATTAATCCTTATGACACCAAGCCTCTGATTGATAAAAAAGAAGATAATGATTCACAAACAGAT
GAAAAAGCTGCAAGCTCAGAAGTAGGAAATAAAAACAAAAAATCTAAAAAAGGCTTGATTATTGGTCTTATTATCCTTCT
TTTAGTTGTTGGCGGTGCTACTTTGGCTTGGGTTGTCTCAACACCAACTAATGTTAAGATACCTAATGTGACAAACTCGA
CACTTAGCCAAGCAAAAAGCAAAATTAAAGATGCAAAACTTAAAGTGGGAACAGTTCATAAACAACAAAGTTCAACGATT
GCTGAAGGAAAAGTTATCAAGACTGATCCAACTAGTGGAACAACCGTGAGATCTAATAGCTCCGTTGATATCTATGTTTC
TACGGGAAATGAAGATATCATCAAAATGAAGGACTTTGTCGGTGAAAAGATTGATGAGGCGATGGCAACATTACTTAAGG
ATTATGGTATTGATGAAAGCCAAGTCACTCAAACTTCCGTTCCTAGTGATTCTTACCCAGCAGGTACAATCATTAAACAA
AGCCCTAAAAAAGGTTCTAGTTTTGATACCAAAGGGAGTGAAAAAATTACCTTTGAAGTTTCAAGTGGAAAACAAGTTGA
AGTTCCGGATTATAAACCGAACGGTCAATATATGACTTATTCACAGTATCAAGCTGCCTTAAAAGCTGCAGGATTCACTA
ATATTACACTAGACCCTCAAGCAACAACGAATCAACAAGCTGATGGTTATGTTTACTCAGTCTATCCAAATGTAGGGACA
TCTGTTGATCCAACTCAAGAAATTGTAGTCACTTATAGCGTCTATACTGCTCCAAGTTCAAGTTCAACAACTTCTGAATC
AACTACGACGTCTGAAACTTCATCAAGTACAACTTCAAGTACTTCAAGCTCTACAACAAGTCAGCCGTCAACAGATAACA
ACAATAGCAGCAAAGAATCTTCAACAACGTCATCAAGTAGTTGA

Domains

Predicted by InterProScan.

• Protein kinase domain (Pkinase) (12-265)

• PASTA domain (PASTA) (365-428)

• PASTA domain (PASTA) (506-573)

• PASTA domain (PASTA) (434-501)

3D structure

• AlphaFold DB: Q9CEF5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pknB	Streptococcus mutans UA159	49.265	86.762	0.427
stkP/pknB	Streptococcus salivarius strain HSISS4	51.445	82.775	0.426
stkP	Streptococcus pneumoniae TIGR4	48.024	80.702	0.388
stkP	Streptococcus pneumoniae D39	47.826	80.702	0.386
stkP	Streptococcus pneumoniae R6	47.826	80.702	0.386
stkP	Streptococcus pneumoniae Rx1	47.628	80.702	0.384