Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   A8140_RS01215 Genome accession   NZ_CP015863
Coordinates   243135..244637 (+) Length   500 a.a.
NCBI ID   WP_005529089.1    Uniprot ID   A0ABY5IIM4
Organism   Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920 strain ATCC 25920 CAIM 519T     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 238135..249637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8140_RS01195 (A8140_01125) hslO 238603..239478 (-) 876 WP_005529081.1 Hsp33 family molecular chaperone HslO -
  A8140_RS01200 (A8140_01130) hslR 239510..239896 (-) 387 WP_005430076.1 ribosome-associated heat shock protein Hsp15 -
  A8140_RS01205 (A8140_01135) gspC 240152..241075 (+) 924 WP_005529083.1 type II secretion system protein GspC -
  A8140_RS01210 (A8140_01140) gspD 241112..243142 (+) 2031 WP_005529086.1 type II secretion system secretin GspD -
  A8140_RS01215 (A8140_01145) pilB/pilB1 243135..244637 (+) 1503 WP_005529089.1 type II secretion system ATPase GspE Machinery gene
  A8140_RS01220 (A8140_01150) gspF 244637..245857 (+) 1221 WP_005430086.1 type II secretion system inner membrane protein GspF -
  A8140_RS01225 (A8140_01155) gspG 245918..246361 (+) 444 WP_005529091.1 type II secretion system major pseudopilin GspG -
  A8140_RS01230 (A8140_01160) - 246393..246998 (+) 606 WP_005529093.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  A8140_RS01235 (A8140_01165) gspI 246979..247374 (+) 396 WP_005529095.1 type II secretion system minor pseudopilin GspI -
  A8140_RS01240 (A8140_01170) gspJ 247397..248077 (+) 681 WP_005529097.1 type II secretion system minor pseudopilin GspJ -
  A8140_RS01245 (A8140_01175) gspK 248070..249080 (+) 1011 WP_005529099.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 500 a.a.        Molecular weight: 55811.02 Da        Isoelectric Point: 5.6449

>NTDB_id=155408 A8140_RS01215 WP_005529089.1 243135..244637(+) (pilB/pilB1) [Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920 strain ATCC 25920 CAIM 519T]
MVDMLDTAPSMRRLPFSFANRFKLVLETEHPERPPILYYVEPLNPAALVEVRRVLKQDFIPQVMDKEAFDKKLTEAYQRD
SSEARQLMEDIGADSDDFFSLAEELPHDEDLLESEDDAPIIKLINAMLGEAIKEGASDIHIETFEKTLSIRFRVDGVLRE
VLTPSRKLAPLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTP
SVHDNFRHLIGRPHGIILVTGPTGSGKSTTLYAGLQEINSNERNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAIL
RQDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCSDCKEP
YEADKEQKKLFGLEEHESLVLHRAKGCEVCNQKGYRGRTGIHELLMVDEKVQELIHSEAGEQEIEKAVRAHSPSIRSDGL
SKVSRGITSLEEVMRVTKEA

Nucleotide


Download         Length: 1503 bp        

>NTDB_id=155408 A8140_RS01215 WP_005529089.1 243135..244637(+) (pilB/pilB1) [Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920 strain ATCC 25920 CAIM 519T]
ATGGTTGATATGTTAGACACCGCGCCAAGTATGCGCCGTCTGCCATTCAGTTTTGCCAATCGCTTTAAACTGGTTTTGGA
GACGGAGCACCCTGAGCGCCCTCCAATTCTGTATTACGTAGAGCCGCTAAACCCTGCGGCTCTGGTTGAAGTACGTCGAG
TACTGAAACAAGACTTTATTCCTCAAGTGATGGACAAAGAAGCGTTCGATAAAAAGCTCACTGAAGCTTATCAACGTGAT
TCTTCTGAAGCCCGTCAGTTGATGGAAGACATTGGCGCAGACAGCGATGACTTCTTCTCATTAGCAGAAGAGCTGCCTCA
CGACGAAGATTTGTTGGAATCGGAAGATGATGCGCCAATCATCAAATTGATTAACGCCATGTTGGGTGAAGCGATCAAAG
AAGGGGCGTCGGATATCCATATTGAGACGTTCGAGAAAACCCTTTCGATTCGTTTCCGTGTCGATGGTGTATTGCGTGAA
GTCTTAACCCCGAGCCGCAAGCTAGCACCTTTGCTAGTTTCTCGTGTGAAGGTCATGGCGAAGCTGGATATTGCGGAAAA
ACGCGTACCTCAAGATGGTCGTATTTCACTGCGTATCGGTGGTCGTGCGGTGGACGTGCGTGTATCGACCATGCCTTCAT
CGCATGGTGAGCGTGTGGTAATGCGTCTACTGGACAAGAACGCCACTCGTCTTGATCTGCATAGTTTGGGTATGACGCCT
TCAGTGCATGACAACTTCCGTCACTTGATTGGTCGTCCTCACGGCATCATCTTGGTGACCGGTCCGACGGGTTCGGGTAA
GTCGACGACCTTGTACGCGGGCTTGCAAGAGATCAACAGCAATGAACGCAACATTCTGACTGTTGAAGATCCTATCGAAT
TCGATATTGATGGCATCGGCCAAACGCAGGTAAACCCTAAGGTTGATATGACCTTTGCGCGAGGTCTGCGTGCGATTCTT
CGTCAAGACCCGGATGTAGTGATGGTCGGTGAGATCCGTGACTTAGAGACAGCACAGATTGCGGTGCAAGCCTCTTTGAC
TGGTCACTTGGTTATGTCGACACTACACACCAACACGGCTGTGGGTGCTATTACTCGTCTGCGCGATATGGGTATTGAAC
CCTTCCTGATCTCTTCTTCGCTATTGGGCGTGTTAGCACAGCGCCTAGTTCGAACCCTTTGTTCTGACTGTAAAGAGCCA
TACGAAGCGGATAAAGAACAGAAGAAATTGTTCGGTCTGGAAGAGCACGAATCGCTGGTTCTGCATCGAGCAAAAGGGTG
TGAAGTATGTAACCAAAAAGGTTACCGCGGTCGTACTGGTATTCACGAACTTCTAATGGTGGATGAGAAAGTTCAAGAAC
TGATCCACAGTGAAGCGGGTGAACAAGAAATTGAAAAAGCCGTACGAGCACACTCGCCAAGCATTCGCAGCGATGGCCTA
AGCAAAGTCAGTCGTGGTATCACTTCTCTTGAAGAGGTGATGCGAGTGACTAAGGAAGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(126-392)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

43.346

100

0.456
  pilB Legionella pneumophila strain ERS1305867

43.788

98.2

0.43
  pilF Thermus thermophilus HB27

44.28

94.4

0.418
  pilB Acinetobacter baumannii D1279779

42.589

95.8

0.408
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

93.8

0.402
  pilB Vibrio parahaemolyticus RIMD 2210633

46.455

81.8

0.38
  pilB Vibrio cholerae strain A1552

47.475

79.2

0.376
  pilB Vibrio campbellii strain DS40M4

45.721

81.8

0.374
  pilF Neisseria gonorrhoeae MS11

46.193

78.8

0.364